Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,741,649 A→T 5.6% intergenic (+71/+1) CJ019_04477 → / ← mqsA Electron transfer flavoprotein‑ubiquinoneoxidoreductase/Antitoxin MqsA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,741,6490AT 5.6% 732.5 / 2.3 255intergenic (+71/+1)CJ019_04477/mqsAElectron transfer flavoprotein‑ubiquinoneoxidoreductase/Antitoxin MqsA
Reads supporting (aligned to +/- strand):  ref base A (116/122);  new base T (15/2);  total (131/124)
Fisher's exact test for biased strand distribution p-value = 1.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

CGGCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTT  >  ACB143/4741514‑4741783
                                                                                                                                       |                                                                                                                                      
cGGCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCGGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTa                                                                                                                                        <  1:301989/136‑1 (MQ=255)
  gCCCCGAACATCACCGGGGACACCCCTGACGGGGGTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCGGCCCTTGCGGCAACAAAAAGCCCCCGGTTCCAATGAGCCGGGGGCTTTTTTTTGGCTGCCATTATc                                                                                                                                      <  8:245012/136‑1 (MQ=255)
  gCCCAGAACATCCCCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAATCCCCCGGTTCTCATGAGCCGGGGGGTTTTTTTTGGCTGCCATTAt                                                                                                                                       >  6:136044/1‑135 (MQ=255)
  gCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGATCTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTGTTGGCTGAGATTAt                                                                                                                                       >  2:31004/1‑135 (MQ=255)
  gCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTACGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATc                                                                                                                                      <  4:1668/136‑1 (MQ=255)
  gCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATc                                                                                                                                      <  4:287095/136‑1 (MQ=255)
  gCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTGTTGGCTGCCATTAt                                                                                                                                       >  1:13383/1‑135 (MQ=255)
  gCCCAGAACAGCTCCTGGGTCACCCCTGAAGGCGCTGGCGGTCCGAACTATCCCAACATTTAAGCCCCCAGCCCTTGCGGCAACAAAACGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATc                                                                                                                                      <  1:371907/136‑1 (MQ=255)
   cccAGAACATCACCTGTGTCACCCCTGAAGGCGTTGGCGGGCCGAACTATCCGAACATGTAAGCCCCCGGCGCTTGCGGCAACAAAACGCCCCCGGATCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTAt                                                                                                                                       <  4:37708/134‑1 (MQ=255)
   cccAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTAt                                                                                                                                       >  6:342900/1‑134 (MQ=255)
    ccAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCCGGTCTCATTAGCCGGGGGGTTTTTGTTGGCTGCCATTATCAg                                                                                                                                    >  6:286170/1‑136 (MQ=255)
     cAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAACCCCCCGGTTCTCATGAGCCGGGGGCTTTTTTTTGGCTGGCATTTTCAgg                                                                                                                                   >  2:145073/1‑136 (MQ=255)
       gAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCa                                                                                                                                 <  5:324755/136‑1 (MQ=255)
         acacCACCTGGGTCACCCGTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTACGCCCCCTGCCCTTGCGGCAACAAAACGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGt                                                                                                                               <  1:188893/136‑1 (MQ=255)
         aCATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGt                                                                                                                               <  7:51304/136‑1 (MQ=255)
           aTCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTAc                                                                                                                             <  1:360683/136‑1 (MQ=255)
           aTCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTAc                                                                                                                             <  3:88742/136‑1 (MQ=255)
           aTCACCTGGGTCACCCATGAAGGCGCTGGCGGGCCGAATTATCCGAACATGTATGTCCGCTGCCCTTGCGGCAACAAAAAGCCCACGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTAc                                                                                                                             <  8:362923/136‑1 (MQ=255)
            tCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACg                                                                                                                            <  2:241425/136‑1 (MQ=255)
            tCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACg                                                                                                                            <  4:108190/136‑1 (MQ=255)
             cACCTGGGTCACCCGTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTATGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACg                                                                                                                            <  6:210983/135‑1 (MQ=255)
                cTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACt                                                                                                                        >  1:211379/1‑136 (MQ=255)
                cTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGGTCTCATGAGCCGGGGGCGTTTTTTTGGCTGCCATTATCAGGCAGTACGCACt                                                                                                                        >  7:49946/1‑136 (MQ=255)
                cTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCCGTTCTCATGAGCCGGGGGCTTTTTTTTGGCTGGCATTATCAGGCAGTACGCCCt                                                                                                                        >  4:189433/1‑136 (MQ=255)
                cTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCAGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAATCCCCCCGGTGTCATGAGACGGGGGCGTTTTTTTTGGTGGCATTTTCAGGCAGGACGCAca                                                                                                                        >  3:137700/1‑135 (MQ=255)
                   ggTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTcc                                                                                                                     <  4:264058/136‑1 (MQ=255)
                     tCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCt                                                                                                                    >  1:290258/1‑135 (MQ=255)
                     tCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTc                                                                                                                   <  2:285608/136‑1 (MQ=255)
                     tCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTc                                                                                                                   <  6:168736/136‑1 (MQ=255)
                     tCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCCGTTGTAATTATCCGGGGGCGGTTTGTTTGCGGCCATTTTCCGGCAGTACGCAcgtact                                                                                                                    >  4:401441/1‑130 (MQ=255)
                     tCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGACCCCGGTTGTCAAGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGGAGTAAGCACTTACt                                                                                                                    >  7:168289/1‑135 (MQ=255)
                     tCACCCCTGAAGGCGCTGGCGGGCCGAAATATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTc                                                                                                                   <  2:88118/136‑1 (MQ=255)
                        cccgTGAAGGCGCTGGGGGGCGGAAATATCCTAAAATTTAAGGCCCATCCCCTTGCGGCTAAAAAACCCCCCGGGTTCTATTTAGCCGGGGGATTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCaaa                                                                                                                <  2:360836/136‑1 (MQ=255)
                          ccTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTTGCATTTTCAGGCAGTACGCAAATCCACaaa                                                                                                                >  5:290867/1‑134 (MQ=255)
                          ccTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTTTTGGCTGCCATTATCAGGCAGTACGCCCTTCCTCAAag                                                                                                               >  8:200433/1‑135 (MQ=255)
                           cTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAagag                                                                                                             >  4:98468/1‑136 (MQ=255)
                           cTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGAGTCTCATGAGCCGGGGGCTTTTTGGTGGCTGGCAGTTTAAGGCAGTACGCACTTACGCAAacag                                                                                                             >  8:149334/1‑136 (MQ=255)
                           cTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAACCCCCCGTTCTCATGAGCCGGGGGCGTTTTTTTTGCTGGCATTATCAGGCACTACGCACATACACAAagag                                                                                                             >  8:334684/1‑136 (MQ=255)
                              aaGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGtt                                                                                                           <  1:349293/135‑1 (MQ=255)
                                ggCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTg                                                                                                        <  8:1066/136‑1 (MQ=255)
                                  cgcTGGCGGGCGGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGAAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGa                                                                                                      <  5:66171/136‑1 (MQ=255)
                                      ggCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCCCTTCCTCAAAGAGTTCTGGATGGc                                                                                                  >  4:231305/1‑136 (MQ=255)
                                      ggCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGc                                                                                                  >  2:323645/1‑136 (MQ=255)
                                      ggCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGc                                                                                                  >  8:190641/1‑136 (MQ=255)
                                      ggCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATAAGGCAGTACGCCCTTCCTCAAAGAGTTATGGATGGc                                                                                                  >  1:127772/1‑136 (MQ=255)
                                      ggCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTATTTTTTGGCTGCCATTAACAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGc                                                                                                  >  8:117842/1‑136 (MQ=255)
                                      ggCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGc                                                                                                  >  5:177066/1‑136 (MQ=255)
                                        cGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCgg                                                                                                >  7:194980/1‑135 (MQ=255)
                                         gggCAGAACTAACCGAACATGTACGGCCCCTGCCCTTGCGGCAACAAAACGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGAt                                                                                               <  8:299095/136‑1 (MQ=255)
                                              gAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCcagc                                                                                          <  3:264041/136‑1 (MQ=255)
                                                       gAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGc                                                                                 <  3:29851/136‑1 (MQ=255)
                                                        aaCATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTAAGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAg                                                                                  >  1:32001/1‑134 (MQ=255)
                                                            tGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGtt                                                                             >  3:352917/1‑135 (MQ=255)
                                                               aaGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAc                                                                         >  3:257391/1‑136 (MQ=255)
                                                               aaGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAc                                                                         >  8:13331/1‑136 (MQ=255)
                                                                aGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAc                                                                         <  5:162233/135‑1 (MQ=255)
                                                                aGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAc                                                                         <  1:236462/135‑1 (MQ=255)
                                                                aGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATAAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAc                                                                         >  7:275297/1‑135 (MQ=255)
                                                                 ggCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTc                                                                                                                   <  3:290073/92‑1 (MQ=255)
                                                                 ggCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAcc                                                                        >  7:166255/1‑135 (MQ=255)
                                                                 ggCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTGTTGGCTGCCATTATCAGGCAGTACGCCCTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGGTTCAcc                                                                        >  2:106797/1‑135 (MQ=255)
                                                                 ggCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTATCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCAcc                                                                        >  7:125970/1‑135 (MQ=255)
                                                                    ccctgccctgGGGGCAACAAAACGCCCCCGGTCCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACg                                                                    <  1:156422/136‑1 (MQ=255)
                                                                     ccTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATAAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGAGATCCAGCAGCTTGAGCAGTTTCACCAACGc                                                                   >  6:306922/1‑136 (MQ=255)
                                                                        gCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCCCTTCCTCAAAGAGTTCTGGATGGCGCTCCAGCAGCTTGAGCAGTTTCCCCAACGccc                                                                 >  1:314054/1‑134 (MQ=255)
                                                                        gCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATAAGGCAGTACGCACTTCCACAAATAGTTAGGGATTGCGCTCCACCAGCGTGAGCCTTTTAACCAACCCCa                                                                 >  6:113950/1‑135 (MQ=255)
                                                                         cccTTGCGGCAACAAAACGCCCCCGGTTCTCATGAGCCTGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCa                                                                 <  1:80049/134‑1 (MQ=255)
                                                                         cccTTGCGGCAACAAAAAGCCCCCGGTTCTGATGAGCCGGGGGCTTTTTTTTTGCTGGCATTTTAAGGCAGTACGGACTTCCTCAAAGAGTTCTGGCTGGCGTTCCACCAGCATGAGCCGTTTCACCAACGCCACt                                                               >  4:318218/1‑136 (MQ=255)
                                                                         cccTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACt                                                               >  5:359172/1‑136 (MQ=255)
                                                                         cccTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACt                                                               >  5:379770/1‑136 (MQ=255)
                                                                         cccTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTGTTGGCTGGCATTTTCAGGCAGTACGCACGTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCCGTTTCACCAACGCCa                                                                 >  4:197953/1‑134 (MQ=255)
                                                                         cccTTGCGGCAACAAAAAGCCCCCCGGTCTAAAGAGCCGGGGGCGTTTTGTTTGCTGCCAATATCAGGCCGTACGCACTTCCAGAAAGAGTTCTTGATGGCGAACCAGCCGCATGAGCACTTTACACAACGCCACt                                                               >  8:80144/1‑136 (MQ=255)
                                                                              gCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCg                                                           <  7:307977/135‑1 (MQ=255)
                                                                              gCGGCAACAAAAACCCCCCGGTTCTCCTGAGCCGGGGGCTTTTTGTTGGCGGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCg                                                           <  4:348343/135‑1 (MQ=255)
                                                                                ggCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCGCAAAGAGTTCTGGATGGCGATGCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCgggg                                                        >  5:264036/1‑136 (MQ=255)
                                                                                  caacaaAAAGCCCCCGGTTCTCATGAGCCGGGGGCGTTTTGTTGGGTGCCATTATCAGGCAGTTCGGACTTCCGGAAAGAGTTGTGGTTGGCGCTCCAGGAGCTTGAGCTGTTTGACCAACCCCACTGGCgggggg                                                      >  6:381195/1‑134 (MQ=255)
                                                                                  caacaaAAAGCCCCCGGTTCTCATGAGCCGGGGGGTTTTGTTTGCTGCCATTATCAGGCAGTACGCCCTTCCGGAAAGAGTTGTGGCTGGCGCTCCAGCAGCGTGCGCAGTTTCACCAACGCCACTGGCGGggtgg                                                     >  2:25820/1‑136 (MQ=255)
                                                                                       aaaaGCCCCCGGTTCTCATGAGCGGGGGGATTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCtt                                                 <  2:194864/136‑1 (MQ=255)
                                                                                            cccccGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGt                                            <  5:196621/136‑1 (MQ=255)
                                                                                               ccGGTTCTCATGCGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGtttt                                         <  3:185579/136‑1 (MQ=255)
                                                                                               ccGGTGCGCATGAGCGGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGtttt                                         <  3:191716/136‑1 (MQ=255)
                                                                                                 ggTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCAATATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCa                                       <  2:345259/136‑1 (MQ=255)
                                                                                                  gTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCaaa                                                                                                                >  6:164/1‑62 (MQ=255)
                                                                                                   ttCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCAt                                      >  6:260454/1‑135 (MQ=255)
                                                                                                   ttCTCATGAGCCGGGGGCTTTTTCTTGGCTGCCAGTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCCCTGGCGGGGTGGTCTTGCCGTTTTCAtt                                     >  3:346953/1‑135 (MQ=255)
                                                                                                        aTGTGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACggg                                <  4:162168/136‑1 (MQ=255)
                                                                                                        aTGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACggg                                <  6:361763/136‑1 (MQ=255)
                                                                                                        aTGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACggg                                <  1:24840/136‑1 (MQ=255)
                                                                                                        aTGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACgg                                 <  4:129779/135‑1 (MQ=255)
                                                                                                         tGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGga                               <  7:218910/136‑1 (MQ=255)
                                                                                                               gggggCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGc                         <  4:25670/136‑1 (MQ=255)
                                                                                                               gggggCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGc                         <  6:183401/136‑1 (MQ=255)
                                                                                                                ggggCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCa                        >  3:278391/1‑136 (MQ=255)
                                                                                                                    gtttttGTTGGCTGCCTTTATCAGGCAGTACGCAATTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGa                    <  3:222133/135‑1 (MQ=255)
                                                                                                                    cTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGc                         <  8:265798/131‑1 (MQ=255)
                                                                                                                          gttgGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCtt                                                                                     >  7:110992/1‑65 (MQ=255)
                                                                                                                          gttgGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCccac              >  7:332320/1‑136 (MQ=255)
                                                                                                                             ggCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGAACCcaccac           >  4:84967/1‑136 (MQ=255)
                                                                                                                             ggCTGCCATTATCAGGCAGTACGCACTTACTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCccacca            <  7:379016/135‑1 (MQ=255)
                                                                                                                                       aTCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGAttt  <  6:93246/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CGGCCCAGAACATCACCTGGGTCACCCCTGAAGGCGCTGGCGGGCCGAACTATCCGAACATGTAAGGCCCCTGCCCTTGCGGCAACAAAAAGCCCCCGGTTCTCATGAGCCGGGGGCTTTTTGTTGGCTGCCATTATCAGGCAGTACGCACTTCCTCAAAGAGTTCTGGATGGCGATCCAGCAGCTTGAGCAGTTTCACCAACGCCACTGGCGGGGTGGTCTTGCCGTTTTCATAACGGGAGAACGCATTGACCCCACCACCGAAGATTT  >  ACB143/4741514‑4741783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: