| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,162 | 0 | G | C | 57.1% | ‑6.5 / 12.5 | 14 | intergenic (+39/‑2420) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base C (8/0); total (12/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑AGGCCGTCA‑GGCCTCCCCGCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACA > ACB143/690050‑690296 | gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCTGGGG‑GGGCCCGAA‑GGGCGCCCCCGCTGG‑TTTTTTGgggtgggtg > 4:35063/1‑134 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGG‑GGGGCGGCG‑GGCCCCCCCCCTGTT‑TTTTTGGGggttgggg > 6:68342/1‑133 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGG‑GGGGCGGCC‑GCCCCCCCCCCGTGG‑TTTTTGGGggttgtgt > 4:315749/1‑132 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGG‑GGGCCGGCG‑GCCCCCCCCCCGTTT‑TTTTTGGGggtgggtt > 7:36487/1‑135 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGGCGGGCC‑GGCGCCCCCGCGTTG‑‑TTTTGTGGggtggttg > 1:247389/1‑135 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑GGGGCGTCA‑GGCCGACCCCCGATG‑GTTTTGGGgggttgttt > 6:355107/1‑134 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑GGGGCGGGAGGGGCCCCCCCCGTGG‑TTTTTGGTggtgggtt > 3:122538/1‑135 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑GGGGCGGGA‑CGCCGCCCCCCGGTG‑TTGGTGGGgggtggggg > 6:103482/1‑132 (MQ=255)gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑GGGGCGGCC‑AGCCCCCCCGCCGGG‑TTTTTTTGgggtggggg > 8:330864/1‑132 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCCGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑AGGCCGTCA‑GGCCACCCCGCTTGG‑GTTTTGGGggttgtgggtg > 3:263525/1‑129 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑GGGGCGGGA‑GGCCGCCCCCCCGTGTTTTTTTGGGGGTGGTTTTTGTGTTTGGgggggacag > 7:350936/1‑132 (MQ=255) cccTTTAAGGTATGTAGTTCACGC‑GGGG‑TGGCCTTCA‑GCCCTCTCCGCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGc < 7:209423/134‑1 (MQ=21) cA‑GACCTCCCCGCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACCACCCTTGCAGTGCCTGTGAGCTAGAGCGCCGCCCGCGCGgcgc > 5:86341/1‑135 (MQ=255) cccGCTTTG‑TTTTTGGGGGGGGGTTGTTGCTGTTGGGGAGCAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGGCCGCCACAACCCTTGCAGTGCCGGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGtctc < 3:171861/135‑1 (MQ=25) gCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTTGTGAGGAACTGTTTTGAGAGGGTGTTGGATGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTTTGAGATCGAGCGCCGCCCGCGCGGAGCTCTGTATcaca > 1:218648/1‑135 (MQ=21) gCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGtttt > 4:239898/1‑47 (MQ=255) gCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGtttt < 3:239898/47‑1 (MQ=255) gCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGc > 2:206105/1‑89 (MQ=255) gCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGc < 1:206105/89‑1 (MQ=255) | GCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGG‑AGGCCGTCA‑GGCCTCCCCGCTTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACA > ACB143/690050‑690296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |