| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,841,920 | 0 | G | C | 50.0% | 4.2 / 14.3 | 26 | intergenic (+9/‑97) | CJ019_04539/hemN | hypothetical protein/Oxygen‑independent coproporphyrinogen IIIoxidase |
| Reads supporting (aligned to +/- strand): ref base G (4/9); new base C (0/13); total (4/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGGGCTGCAAA‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCG > ACB143/4841789‑4842044 | gCCGAAGGGATGAACGCCCTGTTGGGGCGACGCAGCGTGCGTGGGGCAGGGGGTGTGGTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAG‑GGGCCTTTGACGGGGGCTGCaa < 2:241120/135‑1 (MQ=255) gAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGGGGTGCCAa‑gc > 4:294966/1‑135 (MQ=255) tGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGAGCTGCAAA‑GCAGCCCCAATGCCCTTg < 5:284794/135‑1 (MQ=255) gTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGGGGGGCAAA‑GCCGCCCCACAGcccggg > 8:318294/1‑131 (MQ=255) gggcgACGCCGGGTGCGCGTGGCGGGGGGTGTGCGGTTGTTGTTTTGCGTTATCGGGCCCGTCCGGGG‑CCCACACCACCATGGGTTTTT‑GGGCATTTGAGGGGGGCTCAAAA‑GCCGCCCCAATGCCCTTGATACa < 2:243863/134‑1 (MQ=255) cgACGCTGCGGGCGTGGGGCCGGGGGGGGGCTGGTTATGCTATTGGGTAGCGGCCCCCGGCCGGC‑CCCACACCAGCAGGGGCTGTG‑GGGCCATTGCGGGGGGCTGCAAA‑CCAGCCCCAATGCCCTTGATACAAAt < 8:108722/135‑1 (MQ=255) gCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGGGGGGCAAA‑GCCGCCCCCAAGGCCGTGTTACAAATAAacaaa > 2:298420/1‑133 (MQ=255) gggCTGGGGGGGTGGTGGTGATGTTTTTGGTTCTCTGCCCCGGGCCGGG‑CCCACACCCGC‑CGGGGGGTTGGGGCCTTTGCGGGGGGTTCCAAA‑CCCGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTAc < 8:21926/134‑1 (MQ=255) ggCTGGGGGTGGGGTGGTTATGATATTCGGTATCTGGACCCGGCCGGC‑CCCACACCAGCAGGGGGTTTT‑GGGCCATTGTCGGGGGGTCCAAA‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTAc < 7:217489/134‑1 (MQ=255) ggtggtggtgATCCTAGTCGCACTCTCGACCCGGCCGGC‑CCAACCCCACCAGGGGTGGAG‑GGCCCATTGCGGGGGGCTCAAAA‑CCACCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCAt < 4:326987/134‑1 (MQ=255) aTGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGGGCTGGGAA‑TCACCCCCCAAGGCCATGGTTAAAATAAAAACAAGTTGCTTCAAACGGGCCAAGACTCCggg > 2:100649/1‑134 (MQ=255) ggcccccGCCGGGCCCCACCCCAGCAGGGGGGTTT‑GGGCCTTTGCGGGGGGCTCCAAA‑CCCCCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCtt < 8:86593/131‑1 (MQ=255) gcccccGCCGGGCCCCACACCAGCTGGGGCTGCG‑GGCCAATTGCGGGGGGGTCAAAC‑CCCCCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCtt < 6:287710/131‑1 (MQ=255) ccccgcccGGC‑CCCACACCAGAACGGGCTGAG‑GGGCCATTGCGGGGGGCTCAAAC‑CCCCCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCttt < 4:311062/126‑1 (MQ=255) ccccTGCGGGG‑CCCACACCAGCACTGGCTGAG‑GGGCCATTGCCGGGGGCTCAAAA‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCttt < 1:238254/131‑1 (MQ=255) ccccGGCCGGC‑CCCACACCCCCACTGGCGGAG‑GGCCCATTGCCGGGGGCTCCAAC‑CCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCtt < 1:251613/130‑1 (MQ=255) cccTGCGGGC‑CCCACCCCAGCATTGGTTGTT‑GGCCCTTTGTCGGGGGATCAAAC‑CCCGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCgg < 6:275955/135‑1 (MQ=255) cccGGCGGGC‑CCCACACCTGCAGGGGGTG‑T‑GGGGCCTTGGCGGGGGGCGCAAACGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCCGCTCTTTCCgg < 6:100966/135‑1 (MQ=255) gggg‑CCCACCCCAGCAGGGGGTGTT‑GGGCCATTGCCGGGGGGTGCAAC‑CCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTa < 8:209375/134‑1 (MQ=255) ggg‑CCCACACCAGCATTGGGGGTT‑GGCCAATTGCGGGGGGCTCAAAC‑CCCCCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTAc < 7:48866/135‑1 (MQ=255) gg‑CCAACACCAGAAGTGGCTGAT‑GGGCCATTGACGGGGGCTGAAAC‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTAcc < 2:197208/135‑1 (MQ=255) caacacCCGCAGTGGCGGTG‑GGGCATTTGCGGGGGGCGCCAAA‑CCCCCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTc < 7:239576/133‑1 (MQ=255) cagtGGGTGAT‑GGGCC‑TTGCCGGGGGGCGCAAACCCACCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTcgac < 1:281767/132‑1 (MQ=255) cACGGGCTGAT‑GGGCCATTGCGGGGGGCTGCAAA‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTcgac < 7:213842/135‑1 (MQ=255) atgggttGAT‑GGGCATTTGACGGGGGCTCCAAC‑CCACCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTcgacg < 8:328629/129‑1 (MQ=255) aggggggCTGCAAA‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCg < 8:248006/132‑1 (MQ=255) | GCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGG‑CCCACACCAGCACTGGCTGAT‑GGGCCATTGACGGGGGCTGCAAA‑GCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTCCGTTGGGATACCG > ACB143/4841789‑4842044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |