| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,222,510 | 0 | G | C | 34.6% | 31.6 / 12.3 | 27 | intergenic (‑133/+43) | cntO_7/CJ019_04917 | Metal‑pseudopaline receptor CntO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/16); new base C (7/2); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.97e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGTTTACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCA > ACB143/5222377‑5222629 | tGGAAGCAGACCTGTGATGGGGTGATTATTGCGACTCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCg < 8:223426/134‑1 (MQ=255) cGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑t < 5:63280/135‑1 (MQ=255) cGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑t < 1:210371/135‑1 (MQ=255) cGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑t < 5:282835/135‑1 (MQ=255) cAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑c < 1:210533/135‑1 (MQ=255) cccTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TATACCC‑CCCC‑GA‑Cggg > 8:1474/2‑135 (MQ=255) gAGGGGGTGATTATTGCGCATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGtt < 1:202878/134‑1 (MQ=255) gAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCGCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGtt < 6:363174/134‑1 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAACCCCCCCC‑AC‑CGGGGGGTTTa > 2:119542/1‑135 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGGTTAAACCCCCCCA‑AC‑CGGGGGGTTTa > 6:280196/1‑135 (MQ=255) gggggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑GTTACACCCCCC‑AA‑Ccggggggggta > 7:175375/1‑130 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TAACACCCCCCC‑AC‑CGGGGGGGTTAcagg > 3:23020/1‑131 (MQ=255) tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑GTTACCCCCCCC‑AACCGGGGGGTTTGccggg > 1:113326/1‑132 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TAAACCCCCCCC‑AC‑CGGGGGGTTTGCCC‑ggtg > 3:22374/1‑132 (MQ=255) attattGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGG‑TAAAACCCCCCC‑AC‑CCGGGGGTTTGCCC‑g > 2:30828/1‑132 (MQ=255) caatcGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TTAACCCGCCCC‑TA‑CGGGGGGGTTTCCC‑GGGGAGAGGCggggccc > 2:251976/1‑131 (MQ=255) cTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGTTTACCC‑GTGATGTGGCTGGCACAGGCAACTACCGaa < 3:107686/134‑1 (MQ=255) cagggATGCCACGTGGTGTTACTTCACCTGGGCCTGTAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑AAAACCCGCCCC‑TA‑CGGGGGGTTAACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTc < 8:294180/132‑1 (MQ=255) acGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGTTTACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTc < 7:109673/134‑1 (MQ=255) acGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGTTTACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTc < 2:56798/134‑1 (MQ=255) gcggTTCCCTCACCTGTGCCTGTAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑AAAACCCGCCCC‑TA‑GGGGGGGTTTACCC‑GTGATGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAacac < 7:344228/132‑1 (MQ=255) ccTGTGCCTGTATATATTTTTGCTGGGGGGGCCTCTTCGC‑GGG‑AAACCCCGCCCC‑TA‑CGGGGGTTTTACCC‑GTGATTAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg < 6:81681/134‑1 (MQ=255) tccTGTAAATATTTTTGCTGGGGCGGCCTCTTCGC‑GGG‑AACACCCGCCCC‑TC‑CGGGGGGTTTACCC‑GGGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 4:299547/134‑1 (MQ=255) gCCTGTAAATTTTTTTGGTGGGGGGGCCTCTGCGC‑GGG‑TAACCCCGCCCC‑TC‑GGGGGGTTTTCCCC‑GTTAAGAGGGTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 5:273091/135‑1 (MQ=255) gCCTGTAAATATTTTTGCTGGGGCGGCCTCTCCGC‑GGG‑TAAACCCGCCCC‑AA‑CAGGGGGTTAACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 4:350251/135‑1 (MQ=255) gCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGTTTACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAc < 5:185959/135‑1 (MQ=255) gggcggcCTATCCGC‑‑GG‑TAAACCCCCCCCATA‑GGGGGGTTTTACCC‑GTTAATAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGaa < 5:99365/134‑1 (MQ=255) cggggg‑gAAACCCCCCCC‑TT‑CGGGGGTTTTACCCGGTTATTCGGCGGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCca < 5:318881/131‑1 (MQ=255) | TGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGC‑GGG‑TAAACCCGCCCC‑TA‑CGGGGGGTTTACCC‑GTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCA > ACB143/5222377‑5222629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |