| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,164 | 0 | T | G | 52.9% | 0.3 / 12.1 | 19 | intergenic (+41/‑2418) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base G (9/0); total (17/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GG‑CC‑TCCCCGC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGG > ACB143/690035‑690298 | gcTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGCGCCGGAC‑GG‑CC‑CCCCCCC‑TTTG‑tttt > 8:117142/1‑137 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGC‑GG‑CC‑GGCCCGCGTGTG‑TTTTTgggggg > 6:416778/1‑136 (MQ=255) gTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GG‑CCGCCCCCCC‑TTTG‑TTTTTGGgggt‑gggt > 5:202670/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGTCA‑CG‑CC‑GCCCCGC‑GTTGTTTTTTGGGGGT‑GGgttgt > 6:95204/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCGCA‑GG‑CC‑CGCCCCC‑GTGG‑TTTTTGtgggt‑ggggtttt > 2:471824/1‑132 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCCGA‑GG‑CC‑CACCCCC‑CCTG‑TTTTTTTGGGT‑GTgttgtt > 3:199295/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GG‑CC‑GCCCCCC‑GGTT‑TTTTTTTGGGG‑TGgttgtt > 5:253028/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCA‑GG‑CC‑GCCCCGCGGTTG‑TTTTTTTGggt‑gggtttt > 1:268070/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCG‑GC‑CC‑GCCCCGG‑GTGT‑TTTTTTGGGGG‑GGGtttttg > 7:59561/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGAGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGTGGCGGAA‑CG‑CCATCCCCCG‑GTGG‑GTTTTGGTGTT‑TGGttgttg > 6:72910/1‑137 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCG‑GGCCC‑ACCCCGG‑TGTT‑TTTTTGGGGTTGTGGGT‑TGGGTGTTGGggagggtt > 7:202668/1‑134 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GG‑CC‑GCCCCGC‑GTTT‑TTTTTGGGGGT‑TGGTTGTTGGTGTTTGGGGGGGACtt > 2:45241/1‑136 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGGGGGA‑GG‑CC‑GCCCCCCGGTGG‑TTTTTGGGGGT‑GGGGTTTGGGGGTTGGgggggactttt > 2:109724/1‑132 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGGG‑CG‑CCCCCGG‑GTGG‑TTTTTGGGGG‑‑TGGGTGTTGGGGGTGGGGTGGGACGGtt > 2:342970/1‑137 (MQ=255) gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGA‑GGGGAGGCCCTCC‑CG‑CC‑ACCCCCC‑CGTGTTTTTTTGGGGT‑GGGTTTTTG‑TGTTTGGGAGGTACAGTTTTgat > 8:369248/1‑136 (MQ=255) cggcccgg‑CC‑CCCCCGC‑TTTG‑TTTTTGGGGGT‑TGGGTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGGTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGCTCGAGCGCCGCCCGCgcggc < 8:47648/131‑1 (MQ=18) cccccGC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGtctc < 3:415810/136‑1 (MQ=255) cccGC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTcac > 8:366610/1‑137 (MQ=255) gC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAgg > 2:277366/1‑137 (MQ=255) | GCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GG‑CC‑TCCCCGC‑TTTG‑TTTTTGGGGGT‑TGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGG > ACB143/690035‑690298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |