| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,841,912 | 0 | C | G | 53.3% | 11.2 / 20.2 | 16 | intergenic (+1/‑105) | CJ019_04539/hemN | hypothetical protein/Oxygen‑independent coproporphyrinogen IIIoxidase |
| Reads supporting (aligned to +/- strand): ref base C (4/3); new base G (0/8); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTC > ACB143/4841778‑4842031 | ccGGGCTGTCTGCCGAACGGATGAACGCGCTGTTGCGGCGACGCAGCGTGCGAGTGGGAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCGGATGGGCCA‑TTGACgg < 3:357933/137‑1 (MQ=255) ggctggctGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACggggg < 6:115939/137‑1 (MQ=255) gctggctgCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGCTGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGc < 1:446015/137‑1 (MQ=255) cgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGGGGGAAAGCACCCCCCAATCCCATGATAaaaaaaa > 1:84994/1‑134 (MQ=255) cgACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGGGGCAAAGAAGCCCCACAGCCCCTGATACaaaaaa > 1:385460/1‑134 (MQ=255) gCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGGTGGAGATCCGCCCCCAAACCCAACAGACAAAACAacaccat > 5:368547/1‑134 (MQ=255) gTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGCTGGAAAGCAGCCCCCAAGCCCTTGATAAAAATAAACACACTTTACTACa > 6:349288/1‑137 (MQ=255) ggggggtggtggtgATGCTTTTCGTTTTCTGGCCCTTGCGGGCCCC‑TCACCAGCTCTGGCTGTTGGGCCT‑TTGAGGGGGGGTGAAAAGACGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGcc < 4:363610/132‑1 (MQ=255) ggggtgCGGGTTATGTTTTTGGTTATGTGCCCCCTGCCGGGCCC‑ACCCCCGCAGGGGGTTTGGGGCCT‑TTGCGGGGGGGTGAAAAGCCCCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCAt < 2:73027/134‑1 (MQ=255) gggggTTATGCTATGCGGTAT‑GGGGCCCGGGCGGGCCCAACCCCCCCAGTGGCTGAGGGCCCT‑TTCCGGGGGGCTCCAACCCCGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACt < 8:246635/134‑1 (MQ=255) tGGTGATGCTATTCGGTATCTGGCCCCTTCCGGGCCC‑ACACCAGCACTGGATGATGGGCCA‑TAGATGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCACCCCAGTTTCCTAAACACGGCCATAGACAcc > 2:429178/1‑137 (MQ=255) ggTTATGATTTTGGGTAGCGGGCCCCTGCGGGGCCC‑ACACCAGCAGGGGCTG‑TGGGCCATTTGAGGGGGGCGGAAACCCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCg < 7:141737/137‑1 (MQ=255) ggTGTTGCTATTGGGTATCTGCACCCTGCGGGGCCC‑ACCCCACCAGTGGCTGAGGGGCCA‑TTGCGGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCg < 2:84994/137‑1 (MQ=255) tgtcgACCCGGCGGGCCCC‑ACACCAGCTCGGGTTTTGGGGCAT‑TTGAGGGGGGCTCAAACCCACCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCttt < 3:137361/133‑1 (MQ=255) cctggggCTGTTGGGCCT‑TTTCGGGGGGCTGCAACGCCGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTcgacg < 7:454593/132‑1 (MQ=255) ggCGGATGGGCCA‑TTGAGGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTc < 8:455062/137‑1 (MQ=255) | CCGGGCTGGCTGCCGAACGGATGAACGCCCTGTTGCGGCGACGCAGCGTGCGAGTGGCAGGCGGTGTGCTGGTGATGCTATTCGGTATCTGGACCCTGCCGGGCCC‑ACACCAGCACTGGCTGATGGGCCA‑TTGACGGGGGCTGCAAAGCAGCCCCAATGCCCTTGATACAAATCAACACAGTTTCCTACAGACGGCCATAGACTCCGGTGCACTGCTGCTCTTTCCGGGGACTACCCTCATGCTCGACGACCTC > ACB143/4841778‑4842031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |