Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,058,0840CG33.3% 74.9 / 13.5 46D78H (GAC→CAC) proP_7Proline/betaine transporter
Reads supporting (aligned to +/- strand):  ref base C (6/24);  new base G (15/0);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.57e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCA  >  ACB143/5057955‑5058214
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tcAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGGGCCCCCGTTTTTGACCgcag                                                                                                                              >  3:435698/1‑134 (MQ=255)
tcAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCGTTTTTCACCCAtt                                                                                                                              >  7:280616/1‑136 (MQ=255)
 cAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGc                                                                                                                             <  6:400156/137‑1 (MQ=255)
 cAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGc                                                                                                                             <  8:138551/137‑1 (MQ=255)
  aaCATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCg                                                                                                                            <  1:174763/137‑1 (MQ=255)
  aaCATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCGTGGGCCCCCTTTTGTGACCGATGCg                                                                                                                            >  3:335815/1‑137 (MQ=255)
       gACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCaa                                                                                                                       <  8:241124/137‑1 (MQ=255)
         cTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAAtt                                                                                                                     <  3:260770/137‑1 (MQ=255)
            gCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACg                                                                                                                  <  2:271863/137‑1 (MQ=255)
            gCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACg                                                                                                                  <  2:200034/137‑1 (MQ=255)
            gCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCGTTTGTCACCGCTGCGACCCATTACg                                                                                                                  >  2:59553/1‑137 (MQ=255)
             cAGAATTGGAGCTCCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGt                                                                                                                 <  2:197960/137‑1 (MQ=255)
                        cACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGc                                                                                                      <  7:118874/137‑1 (MQ=255)
                             aCGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGGGTGGGCCCCCGTTTGTGACCCCAGCGCCCCATTACGTAGCGACCTAGCGGGCg                                                                                                 >  4:258108/1‑137 (MQ=255)
                                       tctcGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCACTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑aaa                                                                                      <  2:258322/137‑1 (MQ=255)
                                            tATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGc                                                                                 <  6:281034/137‑1 (MQ=255)
                                            tATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCGTTTTTGACCCCAGAGACCAAATAAGTAGATAACTACCGGTACGGGAAAG‑AAACCCGc                                                                                 >  5:336253/1‑137 (MQ=255)
                                            tATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCGTGCGCCCCCTTTTGTCACCGATGCGCCCAAATACGTAGCTACCTAGCGGTAGGGCTTTG‑CAACCCGc                                                                                 >  2:22660/1‑137 (MQ=255)
                                              tGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTTAGCCCCCGATTGTCACCGATACCCCCAAATAAGTAGCTAACTAGACGTCGGGCGATG‑TAAACCGccc                                                                               >  4:417196/1‑137 (MQ=255)
                                               gTTGGAATCAGACCAATACCAATACTGCCGATTGCCAAGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGcccc                                                                              <  3:292515/137‑1 (MQ=255)
                                                    aaTCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGGGTGGGCCCCCGTTTGTGGCCGCTGCGGCCCATTAAGTAGCGAACGAGCGGGCGGGCGTTGTAAACCCCCCCCGaaa                                                                          >  5:299812/1‑137 (MQ=255)
                                                    aaTCAGACCAATACCAATACTGCCGACTGCCATGCAAATAATCGTCAGCAGCATCGCGGGGTTGGGCCCCCGTTTTTGACCGCAGCGCCCAATTACGTAGCTAACAAGCGGTGGGGCGATT‑AATACCCCCCCGGaat                                                                         >  2:102258/1‑136 (MQ=255)
                                                        aGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGGTTGGGCCCCCGTTTGTGACCGATGCGACCAATTAAGTAGCTACCTATCGGTCGGGGGATG‑TAACCCGCCCCGTAaccacc                                                                     >  2:167330/1‑135 (MQ=255)
                                                            caataccaatacTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAAc                                                                 <  3:450735/137‑1 (MQ=255)
                                                                       cTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGGTTGGGCCCCCGTTTTTGACCGCTGCGGCCAATTAAGTAGCTACCTAGCCGTCGGGCGATG‑AAACCCGCCCCCTTAGCAGCGCACGTCTGcaacaa                                                      >  4:163164/1‑137 (MQ=255)
                                                                       cTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTGACCGCTGCGCCCAATTTCGTATCTAACTAGCGGTCGGGCTATG‑AAACCCGCCCCGGAAGCAGCGAACGTGTGcaacaa                                                      >  4:336333/1‑137 (MQ=255)
                                                                       cTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGAACAATTAAGTAGCTACCTAGCGGTCGGGCTATT‑AAACCCGCCCCGTAAGCAGCGAACGTCTGgaacaa                                                      >  8:233280/1‑137 (MQ=255)
                                                                       cTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCGTTTGTGACCGATGCGACCAATTACGTAGCTAACTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCCGCGAACGTCTGcaacaa                                                      >  1:20645/1‑137 (MQ=255)
                                                                        tGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTTGGGCCCCGTTTTTAACCGATTCGACCAATTACGTTGCTTACTATCGGTCGGGCTTTG‑AACCCCGCCCCGGAAACAGCGAACGTCTGCAACAAg                                                     >  8:384026/1‑137 (MQ=255)
                                                                             aTTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTGACCGATGCGCCCCAATAAGTAGCTACCTAGCGGTCGGGCTTTG‑AAACCCGCCCCCGAAGCACCGAACCTCTTCAACAAGGATGt                                                >  5:407292/1‑137 (MQ=255)
                                                                             aTTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCGTGCGGCCCCGTTTGTCACCGCTGGGCCCCATTAACTAGCAAACTAGCGGGCGGGCTATG‑AAACCCGCCCCGGAACCAGCGAACCTCTGGAACAATGCTGt                                                >  8:42808/1‑137 (MQ=255)
                                                                             aTTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCGTGCGCCCCCGTTTTTGACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGGTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGt                                                >  2:100691/1‑137 (MQ=255)
                                                                                       aaaTAATCGTCAGCAGCATCGCGGGCGTGGGCCCCCGTTTGTGACCCATGCGACCAATTACGTTGCTACCTAGCCGTCGGGCGATG‑AAACCCGCCCCGGAAGCAGCGAACGTCTGCAACAAGGATGGCGTTtcatca                                      >  4:320450/1‑137 (MQ=255)
                                                                                                 cagcagCATCGCGGGGGTGGGGCCCCGTTTTTGACCGCTGCGCCCCATTAAGTTGCTACCAAGCGGTCGGGCTATT‑TAACCCGCCCCGTAAGCAGCGCACGTCTGCAACAAGGCTTTGGTTTAATCAACGTTTgggt                            >  4:421037/1‑136 (MQ=255)
                                                                                                  agcagcATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGaa                           <  2:278554/137‑1 (MQ=255)
                                                                                                  agcagcATCGCGGGCTTGCGCCACCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGTaa                           <  2:216188/137‑1 (MQ=255)
                                                                                                       caTCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAaaca                      <  7:236635/137‑1 (MQ=255)
                                                                                                          cgcgGGGGTGCGCCCCCGTTTGTGACCGCTGCGACCCATTTAGGTGGTACCTAGCGGTCGGGCTATG‑GCACACGCCCCGTAAGCCGCGCACGGCGTGAACAAGGAGGGGGGTTCATCAACGGTTGGGTACaaccacg                   >  8:371812/1‑136 (MQ=255)
                                                                                                                       ccccTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGt      <  7:6782/137‑1 (MQ=255)
                                                                                                                         ccTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTTCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAg    <  5:442691/137‑1 (MQ=255)
                                                                                                                         ccTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAg    <  4:434281/137‑1 (MQ=255)
                                                                                                                           ttttGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCa  <  4:435698/137‑1 (MQ=255)
                                                                                                                           ttttGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCa  <  3:421037/137‑1 (MQ=255)
                                                                                                                           ttttGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCa  <  4:281738/137‑1 (MQ=255)
                                                                                                                           ttttGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCa  <  6:259359/137‑1 (MQ=255)
                                                                                                                           ttttGTCACCGATGCGACCAATTACGTAGCTACATAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCa  <  5:7146/137‑1 (MQ=255)
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TCAACATGACTAGCAGAATTGGAGCACCAACGCCAATAGTCTCGTATGTTGGAATCAGACCAATACCAATACTGCCGATTGCCATGCAAATAATCGTCAGCAGCATCGCGGGCTTGCGCCCCCTTTTGTCACCGATGCGACCAATTACGTAGCTACCTAGCGGTCGGGCTATG‑AAACCCGCCCCGTAAGCAGCGAACGTCTGCAACAAGGATGTCGTTTCATCAACGTTTGGGAAAAACAACTTAGCGATGGTCGTAGCA  >  ACB143/5057955‑5058214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑