Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,273,8780CA30.9% 62.7 / 13.6 42D40E (GAC→GAA) fabG_53‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base C (15/14);  new base A (0/13);  total (15/27)
Fisher's exact test for biased strand distribution p-value = 1.15e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGA  >  ACB143/3273747‑3274005
                                                                                                                                    |                                                                                                                               
aTGGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAAc                                                                                                                             <  7:300823/136‑1 (MQ=255)
    aggctgtcATGTCAGAACAACAAAAAGTCGTGCTGGTGAGCGGGGCGTGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTTCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCa                                                                                                                         <  8:79984/136‑1 (MQ=255)
    aggcaggcATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTg                                                                                                                           >  3:43303/1‑134 (MQ=255)
           caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctg                                                                                                                  >  4:300862/1‑136 (MQ=255)
           caTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctg                                                                                                                  <  6:217195/136‑1 (MQ=255)
            aTGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCgctgc                                                                                                                 <  4:321372/136‑1 (MQ=255)
               taaGAACAACAAAAAGTCGGGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGAGAGATCGCCAA‑GCGTTTTTCGCTTTAGGGCTATATTTACTTATTAACAATTTTACATGCCTAAAAACTGCAGCCGctgctg                                                                                                               <  2:154128/133‑1 (MQ=255)
                      aacaaAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGTGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGaaa                                                                                                        >  5:106523/1‑135 (MQ=255)
                       acaaAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATa                                                                                                      >  6:175590/1‑136 (MQ=255)
                           aaaGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACAcg                                                                                                  >  5:76490/1‑136 (MQ=255)
                                cGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCt                                                                                             >  4:366921/1‑136 (MQ=255)
                                    cTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcg                                                                                         <  2:221015/136‑1 (MQ=255)
                                     tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcgg                                                                                        >  3:99004/1‑136 (MQ=255)
                                     tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTggcgg                                                                                        >  5:302402/1‑136 (MQ=255)
                                     tGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GAGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTAGCAAGGCCGACAAACTGCAGCCGATCCTGGAGGAAATACACGCCGATggcgg                                                                                        >  3:270890/1‑136 (MQ=255)
                                                 cgggcgACGCCACGGGCGGCGAGAGCCACAA‑GGGTTTTGCGCGTGCGGGCTAAATTGCCTGCGTACCCCGGCGCCAGGCCGACAAACTCCCGCGGCGGCTGTAGCAAAACCACGCCGCTGGCGGCCAGGCCTACgg                                                                            <  4:107476/136‑1 (MQ=255)
                                                           cagtGGCGGCGAGAGCGCCAC‑GCGTTTTGCGCGTGCGGGCTATATTGCCTGCGTAACCGGGCGCCCGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGctct                                                                  <  8:292809/133‑1 (MQ=255)
                                                           cACTGGGGGCGAGAGCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATGGCCTGCTTAACCCGTCGCCCGGCCGACAAACTGCCGCCGCTGCGGGTGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGctct                                                                  <  1:295573/136‑1 (MQ=255)
                                                           cACGGGCGGCGAGAGCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCTTAACCCGGCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGctct                                                                  <  7:92599/136‑1 (MQ=255)
                                                              ggggggcgAGATCGCAAA‑GGGTTTTGCGCGTTTGGGCTATTTTGCCTGCGAAACCCGGCGCCAGGCCTAAAAACGGCCGCCGGTGGTGGAGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGAt                                                               <  3:208383/132‑1 (MQ=255)
                                                                 cggcgAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcg                                                            >  1:15457/1‑136 (MQ=255)
                                                                  ggctAGATCGCAAA‑GCGTTTTGCGCTTGAGGGCTATATTGCCTGCGAAACCCGCCGCCAGGCCTAAAAACTGCAGCCGCTGCTGTAGCAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcgc                                                           <  1:314836/136‑1 (MQ=255)
                                                                  ggcgATAGCGCCAA‑GCTTTTTGCGGGTGTGGGATATATGGCCTGCTAACCCCGCCGCCTGGCCGAAAAACTCCAGCCGCTGCGGGTGTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcgc                                                           <  4:287109/136‑1 (MQ=255)
                                                                   ggtgtttCGCCAATGCGTTTTGGGCGGGTGGGATTTTTTGCCTGCTTACCCCGGCGCCCGCCCTAAAATCTGCGGCGGGTGGTGGAGTAAAACCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATgcgc                                                           <  4:353930/130‑1 (MQ=255)
                                                                                      ttGCGGGTGTGGGATATATTGCCTGGGTAACCCGGCGCCAGGCCTACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTgg                                         <  2:107633/135‑1 (MQ=255)
                                                                                        gggcgTGTGGGCTATTTTGCCTGCGTACCCCGCCGCCCGGCCTACAAACTGCCGCGGCTGCTGGAGTAAATCCACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                       <  1:170490/133‑1 (MQ=255)
                                                                                        gcggGTGTGGGCTATTTTGCAGGCGAAACCCGGCGCCTGGCGTACAAATTGCGGCGGCGGGTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                       <  7:169391/135‑1 (MQ=255)
                                                                                        gcgcGTGTGGGATATATTGCCTGCGTAACCCGTCGCCTGGCCGAAAAACTCCAGCCGCTGGTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                       <  3:252872/135‑1 (MQ=255)
                                                                                        gcgcGTGAGGGCTTTATTGCCTGCTAACCCCGTCGCCTGGCCTAAAAACTGCCGCCGCTGGTGGTGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                       <  5:68002/135‑1 (MQ=255)
                                                                                        gcgcGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGAAAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                       <  6:302402/135‑1 (MQ=255)
                                                                                        gcgcGTGAGGGCAATATGGCCTGCGTAACCCGTCGCCAGGCCCAAAAACTCCACCCGCTGCTGGAGCAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGcc                                       <  3:221146/135‑1 (MQ=255)
                                                                                         cgcgTGAGGTATATTTTGCCTGCTAAACCGGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGTGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCg                                      <  7:337140/135‑1 (MQ=255)
                                                                                            gTGAGGGCTATATTGCCTGCGAAACCCGGCGCCCGGCCGAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                                  <  7:17525/136‑1 (MQ=255)
                                                                                            gTGAGGGATATATTGCCTGCGTAACCCGTCGCCCGGCCGAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACt                                  <  1:343851/136‑1 (MQ=255)
                                                                                                         tGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATc                     >  3:219310/1‑136 (MQ=255)
                                                                                                           ccTGCGTAACCCGTCGCCAGGCCTAAAAACTGCAGCCGCTGCTGGAGTAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa                   <  8:46623/136‑1 (MQ=255)
                                                                                                           ccTGCGTAACCCGGCGCCAGGCCGACAAACTGCCGCCGCTGCTGGAGCAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGa                   <  1:48899/136‑1 (MQ=255)
                                                                                                              gCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCg                >  1:309180/1‑136 (MQ=255)
                                                                                                                 tAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGATCGTGa             >  7:248586/1‑136 (MQ=255)
                                                                                                                  accccGGCGCCCGCCCTACAAACTGCCGCCGCTGCGGGAGTAAAAACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGa             <  3:366921/133‑1 (MQ=255)
                                                                                                                   aCCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGata           >  1:71102/1‑136 (MQ=255)
                                                                                                                            cAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGa  >  6:241892/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                               
ATGGAGGCAGGCATGTCAGAACAACAAAAAGTCGTGCTGGTGATCGGGGCGGGCGACGCCACTGGCGGCGAGATCGCCAA‑GCGTTTTGCGCGTGAGGGCTATATTGCCTGCGTAACCCGTCGCCAGGCCGACAAACTGCAGCCGCTGCTGGAGGAAATACACGCCGCTGGCGGCCAGGCCTACGGTTTTGGCTCTGATGCGCGCAAGGAAGAGGAGGTGGCCGAACTGGTAGAGACTATCGAGCGTGATATAGGTCCGA  >  ACB143/3273747‑3274005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑