Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT244044,2930T.94.3% 135.3 / 4.6 37intergenic (+124/‑367)oprD_1/czcR_2Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (1/1);  major base . (7/26);  minor base G (2/0);  total (10/27)
Fisher's exact test for biased strand distribution p-value = 6.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTC  >  KT2440/44158‑44402
                                                                                                                                       |                                                                                                             
tCGATTCTGTAATTATCTAAAAGTGAAAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCt                                                                                                               >  7:189444/1‑136 (MQ=255)
    ttCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTGAGTAAAACGAGCATTAAGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttt                                                                                                           >  8:50660/1‑136 (MQ=255)
          aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgc                                                                                                      >  4:179148/1‑133 (MQ=255)
          aaTTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCtttttttt                                                                                                        <  5:281915/133‑1 (MQ=255)
               gCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTaa                                                                                                 <  6:6482/134‑1 (MQ=255)
                   aCTGGGCCAGTTATCCATTGGCGTGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa                                                                                           <  8:217983/136‑1 (MQ=255)
                    cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTGACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa                                                                                           <  5:150997/135‑1 (MQ=255)
                    cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa                                                                                           <  5:16408/135‑1 (MQ=255)
                    cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCa                                                                                           <  5:150996/135‑1 (MQ=255)
                    cTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑‑TTTTTTTGCCTAAAATTCAAg                                                                                         <  8:56660/136‑1 (MQ=255)
                         ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                      <  3:228180/135‑1 (MQ=255)
                         ccAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                      <  1:186611/135‑1 (MQ=255)
                          cAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                    <  7:51451/136‑1 (MQ=255)
                           aGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                    <  4:131763/135‑1 (MQ=255)
                                tCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGa                                                                              >  8:5242/1‑136 (MQ=255)
                                        cGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAc                                                                        <  3:174862/134‑1 (MQ=255)
                                          ggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca                                                                     >  2:200663/1‑135 (MQ=255)
                                                 ttAGTAAAACGAGCATTACGCATTCACGTTGAGTGTCTTAAGTGCTCCTTTGGTAAAAGGTGGATATATGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGGTCGATTGTACGCATCGCATGc                                                             <  7:292279/136‑1 (MQ=255)
                                                 ttAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTATACGCATCGCATGc                                                             <  7:317824/136‑1 (MQ=255)
                                                 ttAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTACGCATCGCATGc                                                             >  8:190628/1‑136 (MQ=255)
                                                     tAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                           <  4:240428/134‑1 (MQ=255)
                                                         aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAg                                                     <  8:189444/136‑1 (MQ=255)
                                                            aGCTTTACGGATTCACCTTGAGTGGATTAAGTGCTACTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTT‑CCTAAAATTCAAGTAGTCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                   <  6:152974/135‑1 (MQ=255)
                                                                 tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgc                                                                                                      >  4:183923/1‑78 (MQ=255)
                                                                 tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCttttttttgc                                                                                                      <  3:183923/80‑3 (MQ=255)
                                                                 tACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                              <  1:200663/135‑1 (MQ=255)
                                                                           ccTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAAt                                    >  7:31549/1‑135 (MQ=255)
                                                                            cTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATg                                   <  2:81335/135‑1 (MQ=255)
                                                                               gAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                <  7:50660/135‑1 (MQ=255)
                                                                                aGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                <  4:292258/134‑1 (MQ=255)
                                                                                   ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCGTTTTTTTTTG‑CTAAAATTGAAGTTGGCGTTCGATTTTTAGCATCGCATTCCCGTGAAGTTCATGACTAACCCAATGACCAGtc                            >  3:170912/1‑135 (MQ=255)
                                                                                       tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTctct                         >  3:249706/1‑134 (MQ=255)
                                                                                       tAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCtt                        >  7:30682/1‑135 (MQ=255)
                                                                                         aGTTCTCCTTTGTTATAAGGTGGATATTTGGCGCCCATTGGGCGTC‑TGTTTTTTGCCTATAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc                       <  6:204017/134‑1 (MQ=255)
                                                                                             cTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAAtgt                  <  5:272960/135‑1 (MQ=255)
                                                                                                 tttGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCCTCGCATGCCGGTGAAGTTCATGACTACCCCAATGACCAGTCTCTTCACTGTGAGCg             >  7:153164/1‑136 (MQ=255)
                                                                                                  ttGGTAAAAGGTGGATATTTGGCGCGCATTGGGCG‑CGGTTTTTTTGCCTAAAATTCAATTAGGCGTTCGATTGTACGCATAGCATGCCGGGGAAGTTCATGACGTCACCAATTACCAGTCTCTTCAATGGGTGCg             >  2:244962/1‑135 (MQ=255)
                                                                                                   tGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg             <  7:190628/134‑1 (MQ=255)
                                                                                                    ggTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg             <  3:229737/133‑1 (MQ=255)
                                                                                                    ggTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCg             <  4:220251/133‑1 (MQ=255)
                                                                                                       aaaaGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc        <  2:239515/135‑1 (MQ=255)
                                                                                                            gTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGt   <  8:31549/135‑1 (MQ=255)
                                                                                                             tGGATATTTGGCGCCCATTGGGCGCGGTTTTTTTTG‑CTAAAATTGAAGTAGGGGTTGGATTGTACGCATCGGAATCCGGTGTAGGTAATGACTACACCAATGACCACGCTCGTCAATTTGGGCGCCCTCGCCggg  >  2:150380/1‑133 (MQ=255)
                                                                                                                                       |                                                                                                             
TCGATTCTGTAATTCGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTC  >  KT2440/44158‑44402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑