Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,393,9460AT25.0% 74.1 / 10.1 56intergenic (+56/‑113)cpoB/queECell division coordinator CpoB/7‑carboxy‑7‑deazaguanine synthase
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base T (13/1);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 4.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGAC  >  KT2440/1393816‑1394067
                                                                                                                                  |                                                                                                                         
cTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCCGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGATGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGGTTGAAtg                                                                                                                      >  8:11192/1‑135 (MQ=255)
 ttCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTAGAAtg                                                                                                                      >  6:222053/1‑134 (MQ=255)
 ttCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTCGTTAGatta                                                                                                                      >  5:56826/1‑132 (MQ=255)
 ttCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTAGGTTGAAtg                                                                                                                      >  3:131859/1‑134 (MQ=255)
 ttCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACACGCGCCAGTCGCGGGGGTTTTCGTTAGattg                                                                                                                      >  4:30618/1‑132 (MQ=255)
  tCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACACGCGCCAGTCGCGGGGTTTTTCGTTAGAATCAc                                                                                                                    >  7:41606/1‑136 (MQ=255)
            gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTTGTATGAATGcccagtt                                                                                                           >  2:199903/1‑131 (MQ=255)
            gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGGTAGGATACCGGCCCgttt                                                                                                           >  2:219919/1‑135 (MQ=255)
                   aGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTaa                                                                                                    <  6:55377/135‑1 (MQ=255)
                     tACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGGGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAac                                                                                                  <  5:207400/135‑1 (MQ=255)
                       ccccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTTGAATCACGGGCCGTTTTTCGTAAacac                                                                                                >  1:8896/1‑135 (MQ=255)
                        cccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTGGTTAGAAAAACTGGCCGGTTTTAGGAAacaccc                                                                                              >  7:142999/1‑134 (MQ=255)
                             ccccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGGTTTAATCAAGGCCCTTTTTTGGTAAACACcctgct                                                                                          >  4:87679/3‑135 (MQ=255)
                             cACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTGGGTAGAATCACTGCCCTGTTTTCGTAAACACCctgctg                                                                                         >  7:49875/1‑136 (MQ=255)
                              aCCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                        <  7:158397/136‑1 (MQ=255)
                               ccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                        <  5:176022/135‑1 (MQ=255)
                               ccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTTTGATGACTGCCCGTTTTTAGTTAACACcctggagc                                                                                        >  2:270565/1‑131 (MQ=255)
                                ctctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCgg                                                                                      <  8:291667/136‑1 (MQ=255)
                                 tctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCgg                                                                                      <  1:203657/135‑1 (MQ=255)
                                 tctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCgg                                                                                      <  1:199903/135‑1 (MQ=255)
                                 tctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCgg                                                                                      <  1:99155/135‑1 (MQ=255)
                                            aCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTAt                                                                            <  2:89531/134‑1 (MQ=255)
                                                        tGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTAGTTAGACTCACTGCCCGTTTTTAGGAAACACCCTGCTGCGGATAACCCTATGGCGAGTCcgcgc                                                               >  6:310267/1‑134 (MQ=255)
                                                        tGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTTGTATGACTGGCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCGATGTCGAGTCcgcgc                                                               >  1:47783/1‑134 (MQ=255)
                                                           ccTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGCAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAg                                                            <  5:222053/135‑1 (MQ=255)
                                                             tGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTCCGTTTTATCACGGCCCATTTTTAGTATACACGCGCGTGCGGGTACCGCGATGTCGACTCcccgcccatg                                                          >  6:194893/1‑129 (MQ=255)
                                                                  aaGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTAGAATAACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGAc                                                    >  7:105019/1‑136 (MQ=255)
                                                                         aGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGGTATAATCCCTGCCGGTTTTTCGTTAACACGCTGCTGCGGATAACGCGATGTCGAGTCCGCGACAGTGGCTGACGGAGGc                                              >  6:42141/1‑135 (MQ=255)
                                                                           gCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCgg                                            <  5:240797/135‑1 (MQ=255)
                                                                           gCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGCGCCTGACGGAGGCGGa                                           >  8:246497/1‑136 (MQ=255)
                                                                            cGTGGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCgg                                            <  5:269071/134‑1 (MQ=255)
                                                                             gtggTCGCTGAACCGCTCCAAACAACCCCGCGCCAGGCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGAc                                          <  6:25643/132‑1 (MQ=255)
                                                                             gTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGAc                                          <  1:53538/135‑1 (MQ=255)
                                                                                 tCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTAGTTAGAATCAATTGCCGTTTTTAGTAAACACCCAGCTGCGGAAAACGCAATGTCGCGTACTCGACCGTGCCTGACGGATGCGGACAGcc                                      >  2:275740/1‑135 (MQ=255)
                                                                                  cGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTGGTTATAATCACGGCCCGTTTTTAGTAAACACGCTGCTGCGGCTAACGCTATGTCGAGTCCGCGCCAGTGCCGGCCGGAGGCGGGCCGCCt                                     >  4:302215/1‑135 (MQ=255)
                                                                                  cGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGGTAGAATCACTGCCCGTTTTTCGTAAACCCGCGGCTGCGGCTAACGCTATGTCGAGTCCGCGCCAGTGCCGGACGGAGGCGGACCGCCTg                                    >  8:190315/1‑136 (MQ=255)
                                                                                   gCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCGTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGCGGCGGACAGCCTg                                    >  3:296076/1‑135 (MQ=255)
                                                                                       tACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTGGGTAGAATCACTGCCCGTTTTTCGTAAACACGCTGCTGGGGTTAAAGCTATGTGGAGTCCGCGCCAGTGCCTGACGGCGGCGGCCAGCCGGGTTat                               >  8:23702/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGTTTTTTGGTTTGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCAc                        >  4:160698/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTTGGGTTTATGACTGCCCGTTTTTGGTTAAAACGCTGCGGGGGGGAACGCGATGTAGCGGCCGCCCCACTTCCTGCCGGCCGCCGGCCGCCTGGTTAGCTGTCAc                        >  6:179639/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTGGTTAGAATCACGGCCCGTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGGCAGTGCCTGACGGAGGCGGGCAGCCGGTTTAGCTGTCAc                        >  2:199423/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTGGTTAGAAACACTGCCCGTTTTTCGTTAACACCCAGCTGCGGGTAACGCTATGTCGCGTCCGCGACACTGGCGGACGGGGGCGCACAGCCAGTGTACGTGTCAc                        >  2:217823/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTGGGTTGGATCACTGCCCGTTTTTCGTAAACACGCGGCTGCGGCTAACGCTATGTGGAGTGCGCGCCCGGGCCGGCCGGCGGCGGCCCGCCGGTTTAGCGGTCAc                        >  4:98989/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTGGGTTGAATCACTGCCCGTTTTTAGTAAACACGCTGCTGGGGCTAACGCGATGTCGAGTACGCGACCGGGCCTTACGGAGGCGGTCAGCCTGTTTAGCTGTccc                        >  6:175855/1‑133 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTGGGTAGAATCACTGCCCTTTTTTGGGTTACACGCTGCGGCGGCTAACGCGTTGTCGAGTGCGCGCCAGTGCCTGGCGGCGGCGGGCAGCCGGGTTAGCTGTCAc                        >  2:303694/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTCGTTAGAATCACTGCCCTTTTTTAGTAAACACGCTGCTGCGGATAAAGCTATGTGGAGTCCGCGGCAGTGCCTGGCCGAGGCGGACAGCCAGTTTAGCGGTCAc                        >  4:109903/1‑135 (MQ=255)
                                                                                               gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTCGGTTGAATCACTGGCCGTTTTTCGTTAACACGCGGCTGCGGATAACGCGATGTCGGGTCCGCGCCAGTGCCGGCCGGCGGCGGGCAGCCTGGTTAGCTGTCACg                       >  8:86623/1‑136 (MQ=255)
                                                                                                      aCCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTgg                <  8:318241/136‑1 (MQ=255)
                                                                                                       cccGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTgg                <  3:302215/135‑1 (MQ=255)
                                                                                                       cccGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGAAAACGCTATGTCGCGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTgg                <  1:219919/135‑1 (MQ=255)
                                                                                                       cccGCGCCAGGCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCGGCTGCGGAAAACGCTAGGTCGAGGCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTgg                <  5:179639/135‑1 (MQ=255)
                                                                                                             caaGTAGCGTGTTTTTTCGTTAGAATCACTGACCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGGCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTTGCTGTCACGCCGGTGGCGAGCAt         <  8:208283/134‑1 (MQ=255)
                                                                                                              cAGTCGCGGGTTTTTTCGTTAGAATCCCTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATg        >  8:177288/1‑136 (MQ=255)
                                                                                                                ggggcgGTTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGc       <  5:23435/132‑1 (MQ=255)
                                                                                                                  ggggggTTTTTTCGTTTAAATCACTCCCCTTTTTTCGTAAACAGGCTGCTGCGGATACCGCTATGTCGAGACCGCGACAGTGCCTGCGGGAGGCGGACAGCCTGTTTAGCTGGCACGCCCGTGGCGAGCATGCa      <  1:275740/131‑1 (MQ=255)
                                                                                                                     gggTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGac  <  3:30618/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                         
CTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGAC  >  KT2440/1393816‑1394067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑