Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,391,0200TG51.8% 4.6 / 15.2 29Q563P (CAG→CCG) bvgSVirulence sensor protein BvgS
Reads supporting (aligned to +/- strand):  ref base T (5/8);  new base G (8/6);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTT  >  KT2440/2390890‑2391132
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ggCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCCGCGCGCGGTCGGCgcgctggcgc                                                                                                             >  8:85251/1‑136 (MQ=255)
 gCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGGCGCGCACATACATGGGGGGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGGCGTTCAGCGCGCGTTCGGCgcgctggcgct                                                                                                            <  8:130874/136‑1 (MQ=255)
     cAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGGGTTAGGCGCgcttgcccgggc                                                                                                         >  6:266832/1‑135 (MQ=255)
                      cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc                                                                                       <  8:141362/136‑1 (MQ=255)
                      cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTGCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc                                                                                       <  8:141440/136‑1 (MQ=255)
                      cAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTGGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc                                                                                       <  8:90581/136‑1 (MQ=255)
                       aaCTTTTCAGGCAACCCTCGCGGTCGCGCTCATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCGGGCGCGGGGGCATCTgccggcgccggc                                                                                       >  3:65800/1‑135 (MQ=255)
                       aaCTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTGCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTgcaggcgcaggc                                                                                       <  4:88375/135‑1 (MQ=255)
                             tCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCAt                                                                                 <  7:47283/135‑1 (MQ=255)
                                          gcgGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGcc                                                                   <  8:311841/136‑1 (MQ=255)
                                              gcgcgCAAATACATGGGGGGGGGCGTACCGTTGCGCAGGGCGCGCATGAATGCCAACTGGGCGTTCCGCGCGGGTTCGGCGCGCGGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGc                                                                <  6:15093/134‑1 (MQ=255)
                                                         catGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACg                                                    <  8:230206/136‑1 (MQ=255)
                                                          tgggggggggggGTACGGTTGAGCTGGGCGCGAATGAAGGCCAACGGGGCGTGCCGCGGGGGTGCGGCGCGGGGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACg                                                    <  6:259972/133‑1 (MQ=255)
                                                                 ggggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCCCGCGGTCGGCGCGCAGGCGCGGGCGAAACTGCAAGCGCAGGCGGGGATTACAACGCCCGGGCAGCAACAAAACCACCGCCCTgg                                             >  4:293674/1‑135 (MQ=255)
                                                                   ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCCCGCGGTCGGCGCGCTGGCCCGGGCGCTAGTGCAGGCGCAGGCCGGCAATCCAGAGCCGGGGCAGCCCCACAACCCCCGCCGGGGccc                                          >  7:295426/1‑135 (MQ=255)
                                                                   ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCCGCGCGCGGTGGGCGCGGGGGCGCGGGGGCATCTGCAGGCGCCGGGGGGGCTTCCAGAGCACGGCCCGCCACAAAAGCACGGCCCTgggc                                           >  3:101404/1‑133 (MQ=255)
                                                                   ggCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCCCGCGGGCGCTGGCGAATCTGCAGGCGCCGGCGGGCATTCCAGCGCACGGCCAGCAACAAAAGCCCGGCCCTGGc                                            >  7:102344/1‑134 (MQ=255)
                                                                     cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCCTTCCAGAGCAGGGCCCGCAACAAAAGCACGGCCGTGGcccg                                         >  4:305536/1‑133 (MQ=255)
                                                                     cGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGGTCGGCGCGCGGGCGCGGGGGCATCTGCAGGCGCCGGCGGGGCTTCCAGAGCAGGGCCCGCAACAAAAGCCCCGCCGTGGCCCGc                                        >  7:296095/1‑136 (MQ=255)
                                                                           gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCGGGCGCGGGCGAATCTGCAGGCGCCGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCCTGGCCAGcccccc                                   >  4:95448/1‑133 (MQ=255)
                                                                           gTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTAAGCCCGCGGTCGGCGCGCTGGCGCTGGGGCATCTGCAGGCGCAGGCGGGCATTGCAGAGCAGGGGCAGCAAAAAAAGCACGGCCGGGGCCCGccccac                                   >  3:200980/1‑135 (MQ=255)
                                                                               aGCAGGGCGCGCATGAATGCCAACTGGTCGTTGAGCGCGCGGTGGGCGCGCGGGCGCAGGCGAATCTTCAGGCGCCAGGGGGCCTTACACAGCACGGCCCCCCAAAAAAGCACCGCCCTGGCCCACACCCCCCGc                               >  6:260830/1‑135 (MQ=255)
                                                                                gCAGGGCGCGCATGAATGCCAACTGGTCGTTCACCGCGCGGTAGGCGCGCAGGCGGAGGCGCATCTGCAGGCGCAGGCGGGCCGTCCCGAGCACGGGCAGCAACAAAACCCCGGCCGTGGGCAGCcccccccccc                              >  6:134793/1‑131 (MQ=255)
                                                                                 cAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTGGGCGCCCGGGCGCTGGCGAATCTTCAGGCCCAGGCCGGCATTGCAGCCCCGGGCCACCAACAAAAGCACCGCCCTGGCCAGCACCACCAGCacg                            >  8:31329/1‑134 (MQ=255)
                                                                                 cAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCCGTCGGCGCGCTGGCGGTTGCGAATGTTCAGGCGCAGGCGGGCATTCCAGATCAGCGCCCCCAACAAAAACACCGCCGTGGCCCGCACCACCaggagg                            >  8:265420/1‑136 (MQ=255)
                                                                                    ggCGCGCATGAATGCCAACTGGTCGTTCAGCCCGCGTTGGGCGCCCTGGCGCTGGCGAATCAGCAGGCGCCGGCGGGCATTCCAGACCCGGGGCAGCCACAAAAGCACGGCCCTGGCCCGCACCACCGGCAGGGcc                         >  7:42486/1‑136 (MQ=255)
                                                                                    ggCGCGCATGAAGGCCACCGGGGCGTGCCGCGCGCGTGGGGCGCGCGGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAACGCACGGCCGTGGCCAGCACCACCTGCAGGGcc                         <  7:25297/136‑1 (MQ=255)
                                                                                            tcaagcccACCTGGTCGTGCCGGGCGCGTTGGGCGCGCGGGCGCTGGCGAAGCTGCAGGCGCAGGCGGGCATTCCAGACCAGGGCCAGCAACAAAACCACGGCCGTGGCCAGCACCACCTGCAGGGCCAggcggc                  <  6:148896/129‑1 (MQ=255)
                                                                                            tGAATGCCAACTGGTCGTTCCGCGCGCGTGCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAACCACGGCCGTGGCCAGCACCACCTGCAGGGCCAggcggc                  <  4:291086/135‑1 (MQ=255)
                                                                                                 gCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCGGGCGCGGGCGAATATGCCGGCGCCGGCGGGCAATACAGAGCACGGGCCCCAACAAAAGCCCGGCCGGGGCCCCCCCCCCCGGCAGGGCCAGGCGGCGGttc             >  4:257728/1‑133 (MQ=255)
                                                                                                           cgtgccgcgcgcGTGCGGCGCGGGGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAACCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGtt  <  7:85251/130‑1 (MQ=255)
                                                                                                           cGTTCCGCGCGGGTGCGGCGCGCGGGCGCTGGCGAATCTGCCGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGt   <  4:65800/135‑1 (MQ=255)
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GGCTTCAAGGTAGCTGTAGTTGCAACTTTTCAGGCAACCCTCGCGGTCGCGCACATACATGGGGTGGGGCGTACCGTTGAGCAGGGCGCGCATGAATGCCAACTGGTCGTTCAGCGCGCGTTCGGCGCGCTGGCGCTGGCGAATCTGCAGGCGCAGGCGGGCATTCCAGAGCAGGGCCAGCAACAAAAGCACGGCCGTGGCCAGCACCACCTGCAGGGCCAGGCGGCTGTAGCCTTTGGAGTT  >  KT2440/2390890‑2391132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑