| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,856,857 | 0 | A | C | 52.9% | 4.8 / 12.3 | 19 | Q287P (CAG→CCG) | hdfR_4 | HTH‑type transcriptional regulator HdfR |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base C (0/9); total (2/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGTTGCGGCCTCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACT > KT2440/2856728‑2856989 | gcggGTTGCGCCAGCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGgcgc < 2:120085/135‑1 (MQ=255) cGGCCTCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGgtg > 4:17574/1‑135 (MQ=255) ccgcgcCCTGGGGGGCGCGCCGCTCATCACCGAGGAATTGGGGGGGCGGGCCCGGCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCACGGGCGTGCACCAGGCGCGCGGGGTGTTGCCGCGGGCCGGCTc < 6:101673/133‑1 (MQ=255) cGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGGGGGCGCGcc > 3:276792/1‑135 (MQ=255) ggggCGAGCCGCTCATCACCGAGGAAAGGGGGGGGCGGGCCGGGGGGGGGCACCCGCGGTTGCACACTCCCCTGACGCTCACGGGCGGGCACCCGGCGCGCTGGGTGTGGCCGCGGGCCGGCGCGCCGGCCCGTc < 2:168293/134‑1 (MQ=255) ggAAATGGGGGTGCGGGCCCGTCGGGGCCACCCGCTGTGGCACACTCACCTGACGCTCACGGGCGGGCACCCGGCGCGCTGGGTGGTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgcc < 7:206201/136‑1 (MQ=255) gacagggtgggGCGGGCCCGGCGGGGGCACCCGCTGGTGCACACTCCCCTGCCGCTCAAGGGCGGGCACCCGGCGCGCGGGGTGTGGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTcgccg < 8:187738/131‑1 (MQ=255) cgggCCCGGCGGGGGCACCCGCTGTGCCACACTCACCTGACGCTCACGGGCGTGCACCAGGCGCGCTGGGTGTGGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTg < 3:258550/133‑1 (MQ=255) ccggtggggggCACCCGGGGTTGCACACTCACCTGCCGCGCAAGGGCGGGCACCCGGCGCGGGGGGGGTGGCCGGGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAgc < 7:195053/130‑1 (MQ=255) gggCACCCGCTGTGCCACACCCACCTCCCGCACACGGGCGTCCACCCGGCGCGCGGGGGGGGCCCGCGGGCGGGCGCGCCGGCCCCTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGc < 7:20624/136‑1 (MQ=255) ggCCCCCGCTGTGCCACACCCCCCTGCCGCGCACGGGCGTGCACTAGGCGCGCGGGGGGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGc < 3:137320/135‑1 (MQ=255) ccccggACGCGCACGGGCGTGCACCCGGGGCGGGGGGTGGGGCCGGGGGCGGGCGCGCCGGCCGGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGAt < 4:11279/130‑1 (MQ=255) cacgggCGTGCACCCGGCGCGCGGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 3:208680/132‑1 (MQ=255) tgggcgggcACCTGGCGCGCTGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 4:156216/132‑1 (MQ=255) cgggCGTGCACCTGGGGGGGGGGGGGTGGCCGGGGGCGGGCGCGCCGCCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATcc < 4:11576/134‑1 (MQ=255) aGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTAt < 4:276792/133‑1 (MQ=255) gCACCCGGCGGGGGGGGTGTGGCCGCGGGCCGGCGGGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTa < 8:80238/136‑1 (MQ=255) aCCCGGCGCGGGGGGGGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTa < 6:97535/134‑1 (MQ=255) ccAGGGGCTCTGGTTTTTGCCGCGGGCCGGGTCGCGGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACt < 3:178843/135‑1 (MQ=255) | GCGCGTTGCGGCCTCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACT > KT2440/2856728‑2856989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |