| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,363 | 0 | A | C | 56.5% | 1.7 / 17.2 | 24 | A24A (GCA→GCC) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base C (13/0); total (19/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > KT2440/3440237‑3440473 | gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCTgggg > 3:46401/1‑134 (MQ=255)gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACACGCCTGGTCGCCGAGGCGCTGCTGCTGGCCCCCCGCCCAGGCGGCGCCCGGGCAGCCTGGgt > 1:118594/1‑135 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGCGGGGTGGCGGGCCACCGCCgat > 5:85695/1‑134 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGGGTTGCCTGCCAACCCCgat > 2:66221/1‑134 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCCCGGGGGGGGCCCCGGCGCCCAGGGTGTGGGAGGCCCCCGccga > 2:259382/1‑131 (MQ=255) ccccTCAACAAGCGGGGGCGCGTGGGGCGGCAGTGCGCGCTCGGGCCCCCGGCGTGGGCGCCGTGGCGCTGCGGCCGGCCGGCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGgc < 8:201843/135‑1 (MQ=255) aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTGGCGGCGCCCGGGGCGCCTGGGGGTGGCCGGCCACCGCCTATAAGGCGGAGcg > 3:20845/1‑134 (MQ=255) aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGGAGGCCCACGCCTATAAGGCGCAGcgc > 8:3460/1‑134 (MQ=255) aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGGAGGCCCACGCCGATAAGGCGCCGCTCGc > 8:293851/1‑136 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGGGGCCCCCGCCGAGAAGGCGCAGCGCGCTgct > 3:294101/1‑133 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCGGGCCACCGCCGATAATGCGCCGCTCGCGGATCAgg > 8:173506/1‑136 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCCGGGTGTGGGCGGCCCACCCCCATAAGGCGCCGCTCGCGGCTCAg > 2:188326/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGTGGGGGCGGCCCCCGCCGCTAAGGCGCCGCGCGCGGATCAGGc > 5:114429/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCCCAGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCACCCCTATAAGGCCCAGCTCGCGGATCAGGcc > 7:28477/1‑136 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCCCGCCGATAAGGCGCCGCTCGCGGATCAGGCCGCTGACGTCGGGAAGCac > 4:79719/1‑134 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACCCCGATAAGGCCCCCCGCGCAGATCAGGCCGATGCCGTCGGCCAGCAGt > 7:38662/1‑136 (MQ=255) ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCCAAGCCGATAATGCCCCGCGCGCTGATCAGGCCGCTGGAGGCGGCCAGCAGtt > 4:217312/1‑135 (MQ=255) gCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCCACGCCGATAAGGCGCAGCTCGGTGAGCAGGCCGAGGACATCGGCGCGCAGTTGGCGGAGAAgg > 3:133325/1‑135 (MQ=255) gcGCCGGGGCGCTGCGGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 7:173506/135‑1 (MQ=255) ggCGCTGCTGCTGGGCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGGAGGCCCCCGCCGATAAAGGGCAGCTCGCGGCTCAGGCCGCTGACTTAGGGAAGCAGTTGGCGGAGAAGGATAGCGCGCCCgg > 6:82568/1‑135 (MQ=255) cgccTGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 3:293589/135‑1 (MQ=255) cgccCGCGCGGCCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa < 1:188326/135‑1 (MQ=255) gggCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTAGGCGGAGAAGGATcgcgc > 6:113183/1‑94 (MQ=255) gggCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTAGGCGGAGAAGGATcgcgc < 5:113183/94‑1 (MQ=255) | GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA > KT2440/3440237‑3440473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |