Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,002,6510GT20.4% 95.0 / 11.1 54S39S (TCC→TCA) CJ019_03781hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/30);  new base T (11/0);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 2.61e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGGTCTGTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCGTGGTGCCCTCTCTACG  >  KT2440/4002524‑4002785
                                                                                                                               |                                                                                                                                      
caGGGTCTGTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTtc                                                                                                                                  <  3:159802/134‑1 (MQ=255)
 aGGGTCTGTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTtctc                                                                                                                                <  6:295249/135‑1 (MQ=255)
      cTGTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCTCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCAGTTACATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTTATTTTGATTTATCATGGt                                                                                                                           >  1:209165/1‑135 (MQ=255)
      cTGTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTTGTTTTCTCATGGt                                                                                                                           >  5:84806/1‑135 (MQ=255)
        gTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCACGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTAATGGTCg                                                                                                                         >  6:99718/1‑135 (MQ=255)
          aaGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTAAGTTGTTCAATGTCGTCTATTTTTGATTTATAAAGGTAGGCt                                                                                                                      >  8:175901/1‑136 (MQ=255)
                tGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTTGATTTATCATGTTAGGCTTCAAg                                                                                                                 >  6:210626/1‑135 (MQ=255)
                tGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAg                                                                                                                 >  2:17097/1‑135 (MQ=255)
                tGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATAGCGCTTTCAGTTGTTCAATGTCGTGTAATTTTGTGTTCTCTTGTTCGGCttaacgc                                                                                                                >  8:144576/1‑131 (MQ=255)
                 gCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTGTAATTTTGATTTATAATTGTCGGCTTCAAGc                                                                                                                >  4:1197/1‑135 (MQ=255)
                 gCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATTGTCGGCTTCAAGCt                                                                                                               >  7:69432/1‑136 (MQ=255)
                  cTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATGCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCt                                                                                                               <  7:263412/135‑1 (MQ=255)
                  cTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCt                                                                                                               >  5:316532/1‑135 (MQ=255)
                  cTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCt                                                                                                               >  2:303770/1‑135 (MQ=255)
                          tgtCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCAc                                                                                                        >  5:140421/1‑134 (MQ=255)
                               ggCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTTAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTTTTTTTGTTTTGTGATTGTGTGCTTGAAGCAGGGCCAcatgctt                                                                                                  >  1:182172/1‑135 (MQ=255)
                               ggCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGTTTTCTCATGGTGGGCTTCAAGCTGCGCCccttcctt                                                                                                  >  3:201073/1‑135 (MQ=255)
                                 cGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCActtcctt                                                                                                  <  1:303770/133‑1 (MQ=255)
                                      tAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATc                                                                                            <  2:61808/134‑1 (MQ=255)
                                       aaaGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTGTATTTTTGTTTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCaa                                                                                          >  5:249399/1‑135 (MQ=255)
                                         aGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAg                                                                                         <  3:300586/134‑1 (MQ=255)
                                           cgcCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCTTCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtc                                                                                     <  7:175901/136‑1 (MQ=255)
                                            gcCTCCTGATCCTCCTTGGGGATCTTTTTTTCGCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtc                                                                                     <  3:202375/135‑1 (MQ=255)
                                             cctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  8:69432/136‑1 (MQ=255)
                                              ctcctGATCCTCCTTTGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  1:241135/135‑1 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  5:37101/135‑1 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  3:110661/135‑1 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  4:156880/135‑1 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  3:1197/135‑1 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  2:200984/135‑1 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCg                                                                                  >  7:36619/1‑136 (MQ=255)
                                              ctcctGATCCTCCTTGGGGAGAATTTTTTCCCGTTCCATCTGTAAAAATTGAGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTAATGGTCGGCTTCAAGCTGCGCCACTTCATTCTGATCAAGTTtctc                                                                                   <  3:165250/135‑1 (MQ=255)
                                                   gATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTGTATTTTTGATTTATCATGGTCGGCTTAAAGCTGCGCCACTTACTTCGGCTCAAGTGTCTCGACCa                                                                              >  2:241135/1‑135 (MQ=255)
                                                   gATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCGTCAAGCTGCGCCACTTCCATCGGATCAAGTGTCTCGACCAg                                                                             >  8:164358/1‑136 (MQ=255)
                                                             ggggAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTGTATTTTTGTTTTGTAATTGTCGGCTTAAAGCTACGCCCCATACATAGGATCAACTGTGTCGACCAGGTAAGgggc                                                                    >  1:152111/1‑135 (MQ=255)
                                                                      tttttCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTggttg                                                           >  2:279421/1‑132 (MQ=255)
                                                                      tttttCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCa                                                          >  8:73596/1‑136 (MQ=255)
                                                                            ccGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTa                                                                          <  8:66785/114‑1 (MQ=255)
                                                                            ccGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCGTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTa                                                                          >  7:66785/1‑114 (MQ=255)
                                                                                tCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  8:43335/101‑1 (MQ=255)
                                                                                tCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   >  7:43335/1‑101 (MQ=255)
                                                                                         aaaaaTTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACt                                         <  6:249399/134‑1 (MQ=255)
                                                                                             aTTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCTCGTGTGGATCATGGAAGATCAAAAAACTTgtgg                                    <  5:210626/135‑1 (MQ=255)
                                                                                                 cgcTTTCAGTTGTTCAATGTCGTGTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCATTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAATAAACTTGTGGTggg                                <  7:269570/135‑1 (MQ=255)
                                                                                                             ttCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATc                   <  8:60543/136‑1 (MQ=255)
                                                                                                                    tcgtcTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCGTGGTGc            <  7:73596/136‑1 (MQ=255)
                                                                                                                     cgtcTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCgtggtg             <  1:14989/134‑1 (MQ=255)
                                                                                                                     cgtcTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCgtggtg             <  6:140421/134‑1 (MQ=255)
                                                                                                                     cgtcTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGGGGTGGGGAGTTCGCTCATCgtggtg             <  2:182172/134‑1 (MQ=255)
                                                                                                                     cgtcTATTTTGGATTTCTCATGGTCGGCGTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCgtggtg             <  1:66369/134‑1 (MQ=255)
                                                                                                                     cgtcTATTTTGGATTTCTCATGGGCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCgtggtg             <  1:98572/134‑1 (MQ=255)
                                                                                                                          aTTTTTGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   >  2:262523/1‑59 (MQ=255)
                                                                                                                          aTTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGtctc                                                                                   <  1:262523/59‑1 (MQ=255)
                                                                                                                            ttttGATTTGTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCGTGGTGCCctc        <  2:209165/132‑1 (MQ=255)
                                                                                                                            tttGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCGTGGTGCCctc        <  6:84806/132‑1 (MQ=255)
                                                                                                                               ggATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCGTGGTGCCCTCTCTACg  <  7:225352/135‑1 (MQ=255)
                                                                                                                               |                                                                                                                                      
CAGGGTCTGTAAGATCTGCTTTGGCGTGTCTGGCGTTGTAAAGCGCCTCCTGATCCTCCTTGGGGAGCATTTTTTCCCGTTCCATCTGTAAAAATTGCGCTTTCAGTTGTTCAATGTCGTCTATTTTGGATTTCTCATGGTCGGCTTCAAGCTGCGCCACTTCCTTCGGATCAAGTGTCTCGACCAGGTAAGGCGCGTGTGGATCATGGAAGATCAAAAAACTTGTGGTGGGGAGTTCGCTCATCGTGGTGCCCTCTCTACG  >  KT2440/4002524‑4002785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑