Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,135,6070AC53.3% ‑2.1 / 12.3 30Q32P (CAG→CCG) tgnC(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (12/2);  new base C (13/3);  total (25/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTC  >  KT2440/4135476‑4135735
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cccGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACCGCCTCAACCCGGCCACCGCCCAGGc                                                                                                                               >  1:265440/1‑135 (MQ=255)
 ccGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCt                                                                                                                             <  7:96266/136‑1 (MQ=255)
  cGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGccgg                                                                                                                            >  8:189635/1‑134 (MQ=255)
       ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGgc                                                                                                                         >  6:312256/1‑134 (MQ=255)
        cTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCAGGgcgc                                                                                                                       >  7:246715/1‑135 (MQ=255)
             cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCTCCCAGGCCTGGGCGCACCTg                                                                                                                  >  6:1642/1‑135 (MQ=255)
             cGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCCCGCCGGGgcgccccggc                                                                                                                 >  8:57554/1‑130 (MQ=255)
                       aaGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCAGCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCg                                                                                                        <  2:21940/135‑1 (MQ=255)
                                gttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCAACTGCCCGCCGCCGCTGAAGcc                                                                                                >  8:73051/1‑134 (MQ=255)
                                 ttgCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCAGGGCGCAACGGCCCGACGCCGATGAAGCCGa                                                                                              >  5:297190/1‑135 (MQ=255)
                                       gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCCACGCCGCAGAAGCCGATGTGGa                                                                                        >  3:236300/1‑135 (MQ=255)
                                            tcgttcgtTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCCCCGCCCACGCCTGGGCGCCCCCGCCCCCCCCCGCAGAAGCCGCGGGGGAGCTgg                                                                                   >  5:170184/1‑135 (MQ=255)
                                            tcgttcgtTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCCCCAGGGCGCAACAGCCCCACGCCGCTGAAGCCCAACTGGAGCTCGc                                                                                  >  7:232140/1‑136 (MQ=255)
                                              gttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGcc                                                                                 >  4:62919/1‑135 (MQ=255)
                                              gttcgttTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCGGGGCGCCACTGCCCGCCCCCGCTGAAACCGCTGTGGAGCTGGCCg                                                                                >  8:128768/1‑136 (MQ=255)
                                                      ccAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGgc                                                                         >  6:70810/1‑135 (MQ=255)
                                                       cAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCCCCGGGGCGCCACAGCCCGACCCCCCAGAAGCCGCTGTGGAGCTGGCCGGTAAGgcc                                                                        >  5:94552/1‑135 (MQ=255)
                                                                ttCGCCGCCCATTGGGGCTTTGCCCAGAGCGCCATGACCTTCGACAGCCTCACCCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGt                                                               <  6:105961/134‑1 (MQ=255)
                                                                aTCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCGGGGGGCCACTGCCCGGCGCCGAGGGAGCCGCTGTGGGGCTGGGCGGTCAGGGCGCCCAGCGTg                                                              >  7:25454/1‑136 (MQ=255)
                                                                  cGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCCCGGCCCGCCGCCGAGGAAGCCGAGGTGGCGCTGGCCGTTCAGGCCGCCCCGCGGGc                                                             >  6:32462/1‑135 (MQ=255)
                                                                     cGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCCCGGCCCGCCGCCGCTGAAGCCGCTGTGGAGCTGGCCGGTCCGGCCGCCCCGCGGGccgt                                                          >  1:71085/1‑133 (MQ=255)
                                                                        ccAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCTGGGCGCCACTGCCCGCCGCCGCTGCAGCCGCTGTGGCGCTGGCCGGTCCGGCCGCCCCGCGGGCCGTCGa                                                       >  3:173577/1‑135 (MQ=255)
                                                                          aaTGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCTGGGCGCCACTGCCCGACGCCGATGAAGCCGCTGTGGAGCTGGCCGTTCAGGCCGCCCCGCGGTCCATCGACa                                                     >  6:69078/1‑135 (MQ=255)
                                                                              gcgcgcTGCCCCGCGCGCCAAGACCTCCCACCCCCCCACCCCCCCCCCCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAg                                                <  7:128768/136‑1 (MQ=255)
                                                                                     gCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCAGGGCGCAACAGCCCGCCGCCGATGGAGCCGATGTGGAGCGGGCCGTTCAGGCCGCCCCCCCGGCCGTCGCCAGCAACGCgggg                                          >  1:84966/1‑135 (MQ=255)
                                                                                              ggCAAGACCTTCGACAGCCTCAACCCGGCCACCCCCCAGGCCTGGGCGCAACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTGACGCCGCCCAGCGTGCCTTCGACAGCAATGCGTGGCGCAGCATc                                 >  4:163795/1‑135 (MQ=255)
                                                                                                           acacCCTCACCCCGGCCCCCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgccc                    <  5:312256/135‑1 (MQ=255)
                                                                                                           aCAGCCTCAACCCGGCCACCGCCCCGGCCAGGGCGCAACAGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCCTGCCTTCGCCACCCAGGCGTGGCGCCGCCTCACCCCTAcccccc                    >  6:304810/1‑135 (MQ=255)
                                                                                                                       cGGCCACCGCCCAGGCCTGGGCGCCACTGCCCGGCGCCGCTGAAGCCGCTGTGGAGCTGGGCGTTCAGGCCGCCCCCCGGGCCGTCGACCGCCAGGCGGGGCGCCGCCTCACCCCGACCGCCCCCGGCCAgctgc        >  6:47295/1‑135 (MQ=255)
                                                                                                                            aCCGCCCAGGCCTGGGCGCAACTGCCCGACCCCGATGAAGCCGATGTGGAGCTGGCCGTTCCCGCCGCCCAGCGTGGCGTCGACAGCAAGGCGGGGCGCACCCTACACGCAAACGCCCCCGGCCCGCTGCTGcgtc  >  8:120609/1‑136 (MQ=255)
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CCCGCACCCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAGCTGCTGCGTC  >  KT2440/4135476‑4135735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑