Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,807,6490GC23.5% 111.9 / 10.8 51A64P (GCC→CCC) mcbCMicrocin B17‑processing protein McbC
Reads supporting (aligned to +/- strand):  ref base G (27/12);  new base C (0/12);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.70e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACATTGGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCT  >  KT2440/4807517‑4807782
                                                                                                                                     |                                                                                                                                     
aCATTGGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGcc                                                                                                                                     <  8:68221/135‑1 (MQ=255)
 cATTGGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGcc                                                                                                                                     >  4:78739/1‑134 (MQ=255)
 cATTGGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCACTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAACCTCAATACC‑ATTGTAGccc                                                                                                                                    >  7:271822/1‑135 (MQ=255)
  aTTGGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGcccc                                                                                                                                   >  7:23232/1‑135 (MQ=255)
     ggCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAg                                                                                                                                <  8:58429/135‑1 (MQ=255)
       cTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCGCCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGc                                                                                                                               >  1:102789/1‑134 (MQ=255)
       atttCCATGCCAAAGTCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGc                                                                                                                               <  4:36477/133‑1 (MQ=255)
             aTGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAAc                                                                                                                         >  1:126009/1‑134 (MQ=255)
                  aaaGGAAACTATACGAACCACAAACCAACTCAACACTTGAGCGCCCTCCACAAAATCCCACCAAACCACCTCTAAAAACTTACCGGAAACGAACAAAAACTCAATACA‑ATTGTAGCCCCAACAAAAAACaaacacc                                                                                                                  <  8:281615/136‑5 (MQ=255)
                    agtcAAATATACTATAAACAAAGCAACTCAACACTTGAGCGCCCTGCACAAAATACCACCAAAAGACCTCTAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCACAAGAAAAAACAAAGAAcc                                                                                                                 <  1:210629/132‑1 (MQ=255)
                            ataCGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCtt                                                                                                          >  5:49845/1‑134 (MQ=255)
                                  tACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACctc                                                                                                   >  2:197259/1‑135 (MQ=255)
                                     acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTATgg                                                                                                >  5:191240/1‑135 (MQ=255)
                                        aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGa                                                                                             >  1:185251/1‑135 (MQ=255)
                                        aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGa                                                                                             >  3:290241/1‑135 (MQ=255)
                                            aaCTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGccc                                                                                         >  2:18580/1‑135 (MQ=255)
                                               tCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCacac                                                                                     >  7:59159/1‑136 (MQ=255)
                                                 accacTTCCGCCCCCTCCACAAAAAACAACAAAAACCCCGCCAAAAACTTCCCGGAAACCAACAAAAACTCAAAACC‑TTTGTACCCCCAAGCAAAAACAAAGACCCGACTCTTACACCTCTGGCGAGCCCACAcc                                                                                    <  3:76957/133‑1 (MQ=255)
                                                 aaCACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACAcc                                                                                    >  2:203033/1‑135 (MQ=255)
                                                  acacTTCAGCGCCCTCCACAAAATCCCACCAAAAGACCTCGAAAAACTTCCCGCAAACCAACTAAAACTCAAAACA‑ATTGAACCCCCAACCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCg                                                                                  <  7:23623/136‑1 (MQ=255)
                                                     cTTCAGCGCCCTGCAACAAAAACCACAAAACCACCTCAAAAAACTTCCCGGAACCCAAATAAAACTCAAAACC‑ATTGAAGCCCCAACAAAAAACAACCAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCt                                                                                <  3:52945/135‑1 (MQ=255)
                                                      ttGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCCCACCCGCTa                                                                               >  1:213487/1‑135 (MQ=255)
                                                      ttGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTa                                                                               >  1:39334/1‑135 (MQ=255)
                                                           cgcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGCCTCTTACACATCTGGCGAGCCCCCACCCGCTACgaa                                                                           >  6:40039/1‑134 (MQ=255)
                                                                tGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATa                                                                     >  1:288068/1‑135 (MQ=255)
                                                                        aaacaaccaAAACACCTCCAAAAACTTACCGCAAACCAACAAAAACACAAAACC‑ATTCTACCCCCAACCAAAAACAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                             <  1:197259/130‑1 (MQ=255)
                                                                          accaccAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGa                                                           >  2:117643/1‑135 (MQ=255)
                                                                               caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAt                                                      >  4:139811/1‑135 (MQ=255)
                                                                                    gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGaa                                                 >  5:159747/1‑135 (MQ=255)
                                                                                    gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGaa                                                 >  5:299458/1‑135 (MQ=255)
                                                                                         cGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACg                                            >  6:56432/1‑135 (MQ=255)
                                                                                            aaaaCTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGccc                                         >  5:146369/1‑135 (MQ=255)
                                                                                             aaaaTTACCGTAACCGAACAAAACCTCAAAACA‑ATTTTACCCCCAACCAAAAAAAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                        <  5:78490/135‑1 (MQ=255)
                                                                                             aaaCTTCCCGGACACGAACTAAAACTCAAAACC‑ATTGTAGCCCCAAACAAAAACAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                        <  2:185251/135‑1 (MQ=255)
                                                                                             aaaCTTCCCGGAACCAAACTAACACTAAATACC‑AATTACCCCCCAACCAAAAACACCCACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                        <  2:101810/135‑1 (MQ=255)
                                                                                                 tacccGCAAACCAACAAAAACTCAAAACC‑ATTGTACCCCCAAGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTc                                    <  3:197330/132‑1 (MQ=255)
                                                                                                  acccGCAAACTAACTAAAAC‑CAAAACCAATTGAACCCCCAACCAAAAACAACCCCCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCa                                   <  2:288068/133‑1 (MQ=255)
                                                                                                      gcaaaCTAACTAAAACTCTAAACC‑TTTTTAGCACCAGCAAAAAACAATCAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                              <  8:125952/134‑1 (MQ=255)
                                                                                                       gAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGAAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                              <  4:290241/135‑1 (MQ=255)
                                                                                                       gAAACCAACTAAAACTCAATACC‑ATTGAAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                              <  6:49845/135‑1 (MQ=255)
                                                                                                       gAAACCAACAAAAACACAATAAC‑ATTGTACCCCCAACCAAAAACAAATAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                              <  6:146369/135‑1 (MQ=255)
                                                                                                       aaaaCCAACAAAAAATAAAACCA‑ATTGTACCCCCAACAAAACAAACACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                              <  2:126009/134‑1 (MQ=255)
                                                                                                           ccaaCTAAAACACTATACA‑ATTGACCCCCCCCGAAAAAAAAATCACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGatat                          <  3:254875/133‑1 (MQ=255)
                                                                                                                tAAAACTCAATACC‑ATTGTACCCCCAAGAAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                    <  8:23232/136‑1 (MQ=255)
                                                                                                                 aaaaCTCAATACC‑TTTGTAGCCCCACCCAAAACCAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                    <  2:53160/135‑1 (MQ=255)
                                                                                                                      tCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAt               <  6:299458/135‑1 (MQ=255)
                                                                                                                           cac‑ATTGTACCCCCCACAAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgctt          <  3:76107/133‑1 (MQ=255)
                                                                                                                             c‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttgc        >  4:78170/1‑135 (MQ=255)
                                                                                                                                   tAGCCCCAAGCAAAAACAAAGCACCGACTCTTACACCTCTGGCGAGCCCCCACCCGCTACGAAGAAATAGTTCATGAGAACGATTTGAAGAACGCCCTCTTCACTTTGATATAGTCCAAGCCTTGCTTGCACCgat  >  8:1812/1‑134 (MQ=255)
                                                                                                                                   tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct  >  8:252586/1‑136 (MQ=255)
                                                                                                                                   tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGc   >  3:304806/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ACATTGGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCT  >  KT2440/4807517‑4807782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑