Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,807,6560GC27.1% 99.6 / 10.2 49S66T (AGC→ACC) mcbCMicrocin B17‑processing protein McbC
Reads supporting (aligned to +/- strand):  ref base G (27/8);  new base C (0/13);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.05e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACT  >  KT2440/4807522‑4807789
                                                                                                                                       |                                                                                                                                     
ggCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAg                                                                                                                                       <  8:58429/135‑1 (MQ=255)
  cTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCGCCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGc                                                                                                                                      >  1:102789/1‑134 (MQ=255)
  atttCCATGCCAAAGTCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGc                                                                                                                                      <  4:36477/133‑1 (MQ=255)
        aTGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAAc                                                                                                                                >  1:126009/1‑134 (MQ=255)
             aaaGGAAACTATACGAACCACAAACCAACTCAACACTTGAGCGCCCTCCACAAAATCCCACCAAACCACCTCTAAAAACTTACCGGAAACGAACAAAAACTCAATACA‑ATTGTAGCCCCAACAAAAAACaaacacc                                                                                                                         <  8:281615/136‑5 (MQ=255)
               agtcAAATATACTATAAACAAAGCAACTCAACACTTGAGCGCCCTGCACAAAATACCACCAAAAGACCTCTAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCACAAGAAAAAACAAAGAAcc                                                                                                                        <  1:210629/132‑1 (MQ=255)
                       ataCGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCtt                                                                                                                 >  5:49845/1‑134 (MQ=255)
                             tACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACctc                                                                                                          >  2:197259/1‑135 (MQ=255)
                                acaAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTATgg                                                                                                       >  5:191240/1‑135 (MQ=255)
                                   aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGa                                                                                                    >  1:185251/1‑135 (MQ=255)
                                   aaGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGa                                                                                                    >  3:290241/1‑135 (MQ=255)
                                       aaCTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGccc                                                                                                >  2:18580/1‑135 (MQ=255)
                                          tCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCacac                                                                                            >  7:59159/1‑136 (MQ=255)
                                            accacTTCCGCCCCCTCCACAAAAAACAACAAAAACCCCGCCAAAAACTTCCCGGAAACCAACAAAAACTCAAAACC‑TTTGTACCCCCAAGCAAAAACAAAGACCCGACTCTTACACCTCTGGCGAGCCCACAcc                                                                                           <  3:76957/133‑1 (MQ=255)
                                            aaCACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACAcc                                                                                           >  2:203033/1‑135 (MQ=255)
                                             acacTTCAGCGCCCTCCACAAAATCCCACCAAAAGACCTCGAAAAACTTCCCGCAAACCAACTAAAACTCAAAACA‑ATTGAACCCCCAACCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCg                                                                                         <  7:23623/136‑1 (MQ=255)
                                                cTTCAGCGCCCTGCAACAAAAACCACAAAACCACCTCAAAAAACTTCCCGGAACCCAAATAAAACTCAAAACC‑ATTGAAGCCCCAACAAAAAACAACCAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCt                                                                                       <  3:52945/135‑1 (MQ=255)
                                                 ttGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCCCACCCGCTa                                                                                      >  1:213487/1‑135 (MQ=255)
                                                 ttGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTa                                                                                      >  1:39334/1‑135 (MQ=255)
                                                      cgcCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGCCTCTTACACATCTGGCGAGCCCCCACCCGCTACgaa                                                                                  >  6:40039/1‑134 (MQ=255)
                                                           tGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATa                                                                            >  1:288068/1‑135 (MQ=255)
                                                                   aaacaaccaAAACACCTCCAAAAACTTACCGCAAACCAACAAAAACACAAAACC‑ATTCTACCCCCAACCAAAAACAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGc                                                                    <  1:197259/130‑1 (MQ=255)
                                                                     accaccAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGa                                                                  >  2:117643/1‑135 (MQ=255)
                                                                          caAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGAt                                                             >  4:139811/1‑135 (MQ=255)
                                                                               gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGaa                                                        >  5:299458/1‑135 (MQ=255)
                                                                               gACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGaa                                                        >  5:159747/1‑135 (MQ=255)
                                                                                    cGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACg                                                   >  6:56432/1‑135 (MQ=255)
                                                                                       aaaaCTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGccc                                                >  5:146369/1‑135 (MQ=255)
                                                                                        aaaaTTACCGTAACCGAACAAAACCTCAAAACA‑ATTTTACCCCCAACCAAAAAAAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  5:78490/135‑1 (MQ=255)
                                                                                        aaaCTTCCCGGACACGAACTAAAACTCAAAACC‑ATTGTAGCCCCAAACAAAAACAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  2:185251/135‑1 (MQ=255)
                                                                                        aaaCTTCCCGGAACCAAACTAACACTAAATACC‑AATTACCCCCCAACCAAAAACACCCACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCct                                               <  2:101810/135‑1 (MQ=255)
                                                                                            tacccGCAAACCAACAAAAACTCAAAACC‑ATTGTACCCCCAAGCAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTc                                           <  3:197330/132‑1 (MQ=255)
                                                                                             acccGCAAACTAACTAAAAC‑CAAAACCAATTGAACCCCCAACCAAAAACAACCCCCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCa                                          <  2:288068/133‑1 (MQ=255)
                                                                                                 gcaaaCTAACTAAAACTCTAAACC‑TTTTTAGCACCAGCAAAAAACAATCAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  8:125952/134‑1 (MQ=255)
                                                                                                  gAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGAAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  4:290241/135‑1 (MQ=255)
                                                                                                  gAAACCAACTAAAACTCAATACC‑ATTGAAGCCCCAACCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  6:49845/135‑1 (MQ=255)
                                                                                                  gAAACCAACAAAAACACAATAAC‑ATTGTACCCCCAACCAAAAACAAATAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  6:146369/135‑1 (MQ=255)
                                                                                                  aaaaCCAACAAAAAATAAAACCA‑ATTGTACCCCCAACAAAACAAACACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTg                                     <  2:126009/134‑1 (MQ=255)
                                                                                                      ccaaCTAAAACACTATACA‑ATTGACCCCCCCCGAAAAAAAAATCACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGatat                                 <  3:254875/133‑1 (MQ=255)
                                                                                                           tAAAACTCAATACC‑ATTGTACCCCCAAGAAAAAACAAACAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                           <  8:23232/136‑1 (MQ=255)
                                                                                                            aaaaCTCAATACC‑TTTGTAGCCCCACCCAAAACCAAACACCCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCa                           <  2:53160/135‑1 (MQ=255)
                                                                                                                 tCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAt                      <  6:299458/135‑1 (MQ=255)
                                                                                                                      cac‑ATTGTACCCCCCACAAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgctt                 <  3:76107/133‑1 (MQ=255)
                                                                                                                        c‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCAttgcttgc               >  4:78170/1‑135 (MQ=255)
                                                                                                                              tAGCCCCAAGCAAAAACAAAGCACCGACTCTTACACCTCTGGCGAGCCCCCACCCGCTACGAAGAAATAGTTCATGAGAACGATTTGAAGAACGCCCTCTTCACTTTGATATAGTCCAAGCCTTGCTTGCACCgat         >  8:1812/1‑134 (MQ=255)
                                                                                                                              tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGct         >  8:252586/1‑136 (MQ=255)
                                                                                                                              tAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGc          >  3:304806/1‑135 (MQ=255)
                                                                                                                                   ccAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTaa    >  7:74561/1‑136 (MQ=255)
                                                                                                                                      aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAAc   >  1:319019/1‑134 (MQ=255)
                                                                                                                                      aGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACt  >  7:165155/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GGCTTTCCATGCCAAAGGCAACTATACGATACACAAAGCAACTCAACACTTGAGCGCCCTGCACCAAATACCACCAAAAGACCTCGAAAAACTTACCGGAAACGAACTAAAACTCAATACC‑ATTGTAGCCCCAAGCAAAAACAAAGAACCGACTCTTACACCTCTGGCGAGCCCACACCCGCTACGAAGAAATAGTTCATGCGAACGATTTGAAGCACGCCCTCTTCACTTTGATATAGTCCAAGCATTGCTTGCACCGCTCTTAACT  >  KT2440/4807522‑4807789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑