Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,689,8160AC55.2% ‑1.4 / 14.5 30S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (6/7);  new base C (6/10);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCA  >  KT2440/5689687‑5689946
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cACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACATGCTCCACAACACCAGCGcc                                                                                                                               >  6:289943/1‑135 (MQ=255)
 aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCAGCcccc                                                                                                                              >  6:51721/1‑135 (MQ=255)
      ggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGGCCATCAGCCACCTCCCCCGCGCCCGGGgc                                                                                                                         >  6:145040/1‑135 (MQ=255)
       gggCCATGGCCAGCGTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGgcgc                                                                                                                       <  8:239057/134‑1 (MQ=255)
           cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCCGCCCGATCACCCGCCCCCCTGGcgcgcg                                                                                                                    >  2:14552/1‑135 (MQ=255)
           cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCCGATCACCCGCCCCCCTGGcgcgcg                                                                                                                    >  2:20240/1‑135 (MQ=255)
                  aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCCGCTCACCCGCGCCCGTGGCGCGCGGCGGCTCg                                                                                                            >  8:196062/1‑136 (MQ=255)
                  aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCg                                                                                                            <  7:3026/136‑1 (MQ=255)
                   gCTGCCGCGGCCTGCCGGGCACCCCGGAGCGCGCCGAACCCGGGGCCCTGGCGCGCAACGTCCGGCTGGTGGAACAGAGGGGGGTGGGTGCGAACTTCCCCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATCg                                                                                                            <  4:289549/135‑1 (MQ=255)
                            gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCACATCACCCCCCCCCCTGGCGCCCGGCGGTTCccccagcccc                                                                                                   >  2:35984/1‑135 (MQ=255)
                                   gggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGAGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGGACATCACGCCCATCACCCGCCCCCGGGGCGCGCCGGGGCTGGCCCAGGCCCACGCCtt                                                                                            >  5:37249/1‑135 (MQ=255)
                                                  cgcCGAAACGGGGGCCCGGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                             <  6:165325/135‑1 (MQ=255)
                                                     cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGGACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCCAGGCCCAGGCCGTGGGCCTGCCGGTAAccgc                                                                          >  4:232840/1‑135 (MQ=255)
                                                       aacccGGGGCCCGGGCGCGAAACAGGCGGGGGGTGCAACCGCGGGGGGGGCGGGCCCACTCCCGCCCCACCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                        <  4:20934/132‑1 (MQ=255)
                                                           cGTGGCCCTGGCGCGCAACCTGCGGCGGGGGCAACAGCGGGGGGGGGGGGCGCACTTCCGCCACAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                   <  7:148951/136‑1 (MQ=255)
                                                           cGTGGCCCTGGCGCCCAACCTGCTGCGGGTGTAACCGGGGGGGGTGCGTGCGCACTTCAGCCATACCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                   <  7:176649/136‑1 (MQ=255)
                                                                     gggcgaAACCTGCTGCGGGTGCAACCGCGCGGGGGGCGGGCGCAGTTCAGCCCGACCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                          <  1:20240/133‑1 (MQ=255)
                                                                     gcgcgaAACCTGCTGCTGGTGCAACCGCGGGGCGGGCGGGCGCACTCCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                          <  1:14552/135‑1 (MQ=255)
                                                                          cacgctgcggcggGTGGAACAGCGGGGGGGGCGGGCGCACTCGACCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                     <  4:171988/131‑1 (MQ=255)
                                                                                    ctgGGGGACCAGAGCGGCGTGCGGGCGCACTGCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACgg                                          <  7:196062/136‑1 (MQ=255)
                                                                                                 gcggggTGCTGGCGCACTCCCCCAAGACCAACTGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGGCGGTAACCGCCGATGTAGCGCTGTACCATCTGATCCTCACGGACGAGTCGCTgcg                             <  7:263700/136‑1 (MQ=255)
                                                                                                  gggcgggcgTGCGCACTTCCGCCATAGCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                             <  6:153741/134‑1 (MQ=255)
                                                                                                     cgtgcgggCGCACATGAGCCAGATCACCAGCGCCCGTGGCGCGCGGCGGATCGCCCACGCCCCCGCCGTGGGCCAGCCGGGAACCCCCGAAGTAGAGCTGTACCACCTGATACTCACGGACGACTCGCTGcgcgc                          >  4:220621/1‑134 (MQ=255)
                                                                                                       ggcgggcgCCCTGCAGCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                        <  4:93804/134‑1 (MQ=255)
                                                                                                       ggcgggcgCACTTCAGCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCGGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                        <  5:51721/134‑1 (MQ=255)
                                                                                                       ggcgggcgCACTCCACCCCGCGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAGCCTCACGGACGAGTCGCTGCGCGAGt                        <  4:20296/134‑1 (MQ=255)
                                                                                                       ggcgagcgTACTTCACCAAGATCACCCGGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCTCTGTACCAGCAGATCCTCACGGACGAGTCGCTGCGCGAGt                        <  2:88662/134‑1 (MQ=255)
                                                                                                              gcACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAg                 <  5:145040/135‑1 (MQ=255)
                                                                                                                       ccAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTACAGCCTGTACCa        >  3:128748/1‑135 (MQ=255)
                                                                                                                             cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTGCTCCAGCCTGTACCACGTGCa  >  1:219835/1‑135 (MQ=255)
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CACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCA  >  KT2440/5689687‑5689946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑