Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,724,3450AC57.7% ‑3.4 / 10.1 29intergenic (‑145/+25)pip/CJ019_05371Proline iminopeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (9/2);  new base C (15/0);  total (27/2)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGAGGATTATTCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGCCGATAGCGTCGCCGCCTCCACTCAACGCTTGTAACGCTCGTCGCCCCAGGCCAGCAGCGCTTGCAGTAGCTGCCTCAATACCGCTTGCGTCGGTTGCGCCAAGTCTTCCCGGTAGGCAAAAGGCTCGAC  >  KT2440/5724214‑5724477
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tgAGGATTATTCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGc                                                                                                                                   <  4:53728/135‑1 (MQ=255)
tgAGGATTATTCGGCCGTGGGCCATAATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGgcgc                                                                                                                                   >  5:286569/1‑135 (MQ=255)
  aGGATTATTCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGccgccgc                                                                                                                                >  8:57019/1‑135 (MQ=255)
  aGGATTATTCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCCCCg                                                                                                                                 >  3:243840/1‑135 (MQ=255)
  aGGATTATTCGGCCGTGGGCCATCATAAACCTGAATTGCGCTATCAGCACCTTCCTTGCAAACGCGTTCTATCGGACCCAATTCACACATGCGCAGAATTCCGGCAACTGCCTCCCACGCTGCCAGGCAAccccct                                                                                                                                >  8:107676/1‑134 (MQ=255)
     attattCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCCCGGTGCCTGGCGACGCCGAtt                                                                                                                              >  6:312153/1‑134 (MQ=255)
       tattCGGCCGTGGGCCGTCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTGCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCGCCCCCGGTGCCTGGCGCCGCCGATCGc                                                                                                                            >  4:254452/1‑135 (MQ=255)
       tattCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGCCGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGCATTCCGGCCACTGCCTCCCACGGTGCCTGGGGGCGCCgcgggc                                                                                                                            >  3:176196/1‑130 (MQ=255)
       tattCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACCCCGAAAGc                                                                                                                            >  1:272182/1‑135 (MQ=255)
           cGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCACCTGCCTCCCACGGTGCCTGGCGCCGCCGCTATCGtgg                                                                                                                        >  5:64478/1‑133 (MQ=255)
                 tGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGCCGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGCATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGCCGCTAGCGTCgccgccg                                                                                                                  >  3:66671/1‑134 (MQ=255)
                 tGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGGCGCCGATAGCGTCgccgccgc                                                                                                                 >  7:25761/1‑134 (MQ=255)
                 tGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCGCCGCCt                                                                                                                  >  4:273271/1‑135 (MQ=255)
                 tGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCCGCGCCGCTAGCGGCgccgccg                                                                                                                  >  1:2494/1‑134 (MQ=255)
                    gCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCGCCGCCTcc                                                                                                                >  5:77603/1‑134 (MQ=255)
                           tAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGATAGCGTCGCCGCCGCCCCCCAACGc                                                                                                       >  7:49953/1‑136 (MQ=255)
                                      tGCGCTTTCAGCACCGTCGTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGATAGCGTCGCCGCCTCCACTCAACGCTTGTAACGCt                                                                                             >  4:239340/1‑135 (MQ=255)
                                      tGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCGCCGCCGCCCCTCAACGCGTGTAACGCt                                                                                             >  1:10245/1‑135 (MQ=255)
                                      tGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCGCCGCCGCCACTCCACGCGTGTAAAGcg                                                                                             >  4:247816/1‑134 (MQ=255)
                                      tGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGCCGCTAGCGTCGCCGCCGCCACTCACCGCTTGTAAAGCtc                                                                                            >  7:252662/1‑136 (MQ=255)
                                      tGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCCCCGCCGATAGCGTACCCGCCTCCCCACAACCCTTGttaaagt                                                                                             >  1:214495/1‑129 (MQ=255)
                                           tttCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGATAGCGTCGCCGCCGCCCCTCCACGCTTGTAACGCTCGTCGc                                                                                       >  7:185745/1‑136 (MQ=255)
                                                       ccTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCCCCGCCGGCCCGCAACGCGTGTAACGCTCGTCGCCCCAGGCcagc                                                                            >  4:286188/1‑135 (MQ=255)
                                                         ttGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGATAGCGTCGCCGCCGCCCCTCAACGCGTGTAACGCTCGTCGCCCCAGGCCcgcagc                                                                         >  7:21630/1‑136 (MQ=255)
                                                                  cgTTCGAACGGCGCGAATTGACCCTTGAGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGCCGATAGCGTCGCCGCCTCCACTCAACGCTTGTAACGCTCGTCGCCCCAGGCCAGAACCGCATGCAg                                                                 >  8:190056/1‑135 (MQ=255)
                                                                   gTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCGCCGCCGCCCCTCAACGCGTGTTACGCGCGTCGCCCCCGGCCCCCCGCGCGTGCAgg                                                                >  1:93637/1‑134 (MQ=255)
                                                                         aCGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGCCGCCGCTAGCGTCGCCGCCTGCCCTCAACGCGTGTTACGCTCGTCGCCCCCGGCCAGCAGCGCGTGCAGTAggggg                                                          >  1:167554/1‑131 (MQ=255)
                                                                                  aTTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGCCGATATCGTCGCCGCCGCCCCTCAACGCTTGTAACGCTCGTCGCCCCAGGCCAGCCGCGCTTGCAGTAGCTGCCTCAATAcc                                                 >  2:259503/1‑135 (MQ=255)
                                                                                                          cAACTGCCTCCCACGGTGCCTGGCGACGCCGATAGCGTCGCCGCCTCCACTCAACGCTTGTAACGCTCGTCGCCCCAGGCCAGCAGCGCTTGCAGTAGCTGCCTCAATACCGCTTGCGTCGGTTGCGCCAAGTCt                         >  1:184657/1‑135 (MQ=255)
                                                                                                                             cTGGCGACGCCGATAGCGTCGCCGCCGCCACGCAACGCGTGTTAAGCTCGTCGCCCCAGGCCCGCAGCGCTTTCCGTTGCTGGCTCAATACCGCGTGCGTCGGTTGCGCGCAGTGTTACCGGTAGGCCAAAGGcgc     >  8:21172/1‑134 (MQ=255)
                                                                                                                                 cGAAGCCGATAGCGTCGCCGCCTCCACTCAACGCTTGTAACGCTCGTCGCCCCAGGCCAGCAGCGCTTGCAGTAGCTGCCTCAATACCGCTTGCGTCGGTTGCGCCAAGTCTTCCCGGTAGGCAAAAGGCTCGAc  <  3:297563/135‑1 (MQ=255)
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TGAGGATTATTCGGCCGTGGGCCATCATAAACCTGAATTGCGCTTTCAGCACCGTCCTTGCTGACGCGTTCGAACGGCGCGAATTGACCCTTGCGCAGAATTCCGGCAACTGCCTCCCACGGTGCCTGGCGACGCCGATAGCGTCGCCGCCTCCACTCAACGCTTGTAACGCTCGTCGCCCCAGGCCAGCAGCGCTTGCAGTAGCTGCCTCAATACCGCTTGCGTCGGTTGCGCCAAGTCTTCCCGGTAGGCAAAAGGCTCGAC  >  KT2440/5724214‑5724477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑