| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,076,448 | 0 | T | G | 57.9% | 2.2 / 12.4 | 19 | T141P (ACC→CCC) | purK | N5‑carboxyaminoimidazole ribonucleotidesynthase |
| Reads supporting (aligned to +/- strand): ref base T (5/3); new base G (1/10); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCAGCGACACCTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGC > KT2440/6076315‑6076576 | accaGCGACACCTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTg < 4:173197/135‑1 (MQ=255) ccaGCGACACCTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGACTTCTGGCGAGCGCAGCACCTTCAGGCCCATGCCCTCCTAACCCACGGTGCg > 8:38352/1‑136 (MQ=255) cGACACCTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGt > 2:162198/1‑135 (MQ=255) ccTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAg < 8:211388/136‑1 (MQ=255) cTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCCTAACCCAGGGTGCGGGTTTTCAg > 5:149017/1‑135 (MQ=255) cTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTGCTGGCCCTTGGCGTCGTAACCCAGGGGGCGGGGTTTctg > 3:43126/1‑133 (MQ=255) cGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTAAGCAcgg > 6:194445/1‑135 (MQ=255) aaCGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCAcggccgggg > 8:177062/1‑134 (MQ=255) agcagGCAGGGCACGCTGCCCTGCGCGGCGAAGGTACCCACCACGTCTTCTGGCGGGCGCAGCACCTTCTGGCCCTGGCCGGCGAAACCCCGGGGGCGGGTTTCCCGCACGGCCGGCCGGCCTATGCTGGCCACt < 3:107591/135‑1 (MQ=255) ccgcggggCGAAGGGACCCACCACGTCTTCGGGGGGGCGCAGCACCTGCGGGCCCTGGCGGGCGTACCCCCGGGGGGGGGTTTCCCGCCCGGCCGGCAGGCCGAGGCTGGCCACTGCGGCGTCCAGATCGGCCTg < 2:108974/129‑1 (MQ=255) caccacGTCTTCTGGCGTGCGCAGCACCGGCTGGCCCTTGCCGTCGTAACCCCGGGTGCGGGTTTGCAGCACGGCCGGCAGGCTGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGaa < 6:99118/135‑1 (MQ=255) agttcttctGGCGGGCGCAGCACCTTCTGGCCCTTGCGGTCGTACCCCTGGGGGCGGGTTTGCAGCAGGGCCGGCAGGCGGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCg < 7:78853/133‑1 (MQ=255) ggcgcAGCACCTTCTGGCCCTTGCCGTCGTACCCCCGGGGGCGGGTTTTCCGCCCGGCCGGCCGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGc < 5:166995/134‑1 (MQ=255) gcgcCGCACATGCGGGCCCTGGCTGCCGAAACCCAGGGGGCGGTTTTACCCCCCGCCCGCCCGCCCGTTGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGcc < 5:175447/135‑1 (MQ=255) ttCTGGCCCTGGCCGGCGTAACCCCGGGGGCGGGTTTGCAGCAGGGCCGGCAGGCGGTTGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCgg < 3:240333/135‑1 (MQ=255) ggCCCTTGCCGTCTAACCCCCGGGGGCGGGTTTTCCGCACGGCCGGCAGGCCTATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACa < 6:315136/135‑1 (MQ=255) ggcttcaCCCCGGGGGGGGGTTTGCAGCCCGGCGGGCCGGCGGAGGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTc < 1:262886/129‑1 (MQ=255) gcgcaaCCCAGGGGGCGTTGTTTCAGCACGGCGGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCGTGCGAGAGGATGTCGGCGAAGGGCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCg < 6:149017/131‑1 (MQ=255) ccAGGGGGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGc < 6:80656/135‑1 (MQ=255) | ACCAGCGACACCTCGCCGGTGAACGGCACGAAGCCCTCCAGCAGGCATGGCACGCTGCCCAGCTCGGCGAAGGTACCCACCACGTCTTCTGGCGTGCGCAGCACCTTCTGGCCCTTGCCGTCGTAACCCAGGGTGCGGGTTTTCAGCACGGCCGGCAGGCCGATGCTGGCCACTGCGGCGTCCAGATCGGCCTGCGAGAGGATGTCGGCGAAGGCCGGGGTGGGGATGCCCAGGTCGCGGAACATGCTCTTTTCGAACAGGC > KT2440/6076315‑6076576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |