Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	179,484	T→C	C316R (TGC→CGC)	CJ019_00182 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	179,484	0	T	C	100.0%	237.2 / NA	70	C316R (TGC→CGC)	CJ019_00182	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/56);  total (14/56)

TCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  KT2440/179360‑179614                                                                                                                             |                                                                                                                                   tccgggCCTGGCGGGCCGGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTGGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGGGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                          <  6:36121/132‑1 (MQ=255)    gggCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcgg                                                                                                                       <  2:83769/135‑1 (MQ=255)     ggCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGGGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcgg                                                                                                                       <  8:218266/134‑1 (MQ=255)      gCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcggcggcg                                                                                                                     <  3:83708/135‑1 (MQ=255)         tggctggcATGAACGCCCGCCTTGGCGGCCAGAGTACGGACCAGTTGTTGCAAGCGTTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCGCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  8:292557/136‑1 (MQ=255)           gctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACGAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  8:198488/134‑1 (MQ=255)            ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                 <  4:148321/133‑1 (MQ=255)             tggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAACCCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGttt                                                                                                                >  1:178927/1‑133 (MQ=255)               gcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAg                                                                                                            <  3:2144/135‑1 (MQ=255)                  tGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGc                                                                                                         >  5:43464/1‑135 (MQ=255)                   gAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCt                                                                                                        >  5:299286/1‑135 (MQ=255)                    aaCGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTg                                                                                                       <  8:99859/135‑1 (MQ=255)                    aaCGCCCGCCGTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTgg                                                                                                      <  8:132014/136‑1 (MQ=255)                       gcccgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCa                                                                                                    >  4:312175/1‑135 (MQ=255)                          cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  8:246960/136‑1 (MQ=255)                          cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  8:39218/136‑1 (MQ=255)                          cgccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  8:269052/136‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCATCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:238172/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               <  7:83734/136‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  5:227915/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  4:194882/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:133433/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:243883/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  6:32638/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:87753/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  2:269838/135‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGGGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                <  3:147895/135‑1 (MQ=255)                            ccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                               >  4:141401/1‑135 (MQ=255)                            ccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgg                                                                                                >  2:139843/1‑134 (MQ=255)                             cTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGc                                                                                              >  3:52397/1‑135 (MQ=255)                                ggcggcCAGCGTACCGACCAGTGGTGGCAAGCGGTAGGTGAACAGCTGCGTCGACCTTGGGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATg                                                                                           <  2:284340/135‑1 (MQ=255)                                     ccAGCGTACCGACGAGTTTTTGCAAGGGGTAGGTGAACTGCTTCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCa                                                                                      <  5:38781/135‑1 (MQ=255)                                     ccAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCa                                                                                      <  4:52397/135‑1 (MQ=255)                                       aGCGTACCGCCCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGGCGTACGTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCagg                                                                                    <  1:244156/135‑3 (MQ=255)                                        gCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                   <  6:43464/135‑1 (MQ=255)                                             ccGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGaggagg                                                                              <  8:76170/135‑1 (MQ=255)                                              cGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGaggagg                                                                              <  5:116816/134‑1 (MQ=255)                                              cGACCAGTGGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGaggagg                                                                              <  1:307850/134‑1 (MQ=255)                                                      tgttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTg                                                                                                       <  4:252425/101‑1 (MQ=255)                                                      tgttgCAAGCGGTAGGAGAACAGCAGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTg                                                                                                       >  3:252425/1‑101 (MQ=255)                                                        ttgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCtt                                                                   <  3:312175/135‑1 (MQ=255)                                                         tgCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  6:260752/135‑1 (MQ=255)                                                         tgCAAGAGGTAGGTTAACAGCTGCGTCGTACTTGTGCCAGCTACAAGGAAACCAACGACCTCATTTCACGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  2:137509/135‑1 (MQ=255)                                                         ggCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  5:82023/134‑1 (MQ=255)                                                          gCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCCACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGc                                                                 <  1:272199/135‑1 (MQ=255)                                                             aGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGccccg                                                             >  7:248216/1‑134 (MQ=255)                                                                  tAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGctct                                                        >  8:191263/1‑136 (MQ=255)                                                                       gAACAGCGGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                   <  7:289032/136‑1 (MQ=255)                                                                               gCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGtt                                            >  5:287294/1‑135 (MQ=255)                                                                                  tcgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCag                                        <  7:86038/136‑1 (MQ=255)                                                                                   cgtACTTGTGCCAGCTACCCGGAAACCAACGTCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCag                                        <  5:231029/135‑1 (MQ=255)                                                                                     tACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCagag                                      >  6:303776/1‑135 (MQ=255)                                                                                        ttGTGCCAGCTACCCGGACACCACCGACCTCATTACCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCTTGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                   <  2:310567/135‑1 (MQ=255)                                                                                        ttGTGCCAGCTACCCGGAAACGAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                   <  4:177739/135‑1 (MQ=255)                                                                                        ttGTGCCAGCTACCCGGAAACCCACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                   <  2:306418/135‑1 (MQ=255)                                                                                             ccAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  <  7:115003/99‑1 (MQ=255)                                                                                             ccAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTg                                                                  >  8:115003/1‑99 (MQ=255)                                                                                                gCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGaaa                           >  3:48520/1‑135 (MQ=255)                                                                                                    cccGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACggg                       <  4:48520/135‑1 (MQ=255)                                                                                                    cccGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACggg                       <  8:55566/135‑1 (MQ=255)                                                                                                     ccGGAAACCACCGTCCTCATTTCCCGCAGCCGCGGCGGCTAGTTTGCAGTGCTGGGACCGGGCATGGTGCATGAGGAGGCAGTCCACCTTGCCCAGGCTCTGGAGGCAACGTTGAAGAGCCTGCACGAAACgg                        <  2:105260/133‑1 (MQ=255)                                                                                                             cAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGa              <  2:198709/135‑1 (MQ=255)                                                                                                                        tttCCCGCAGCCGCGGCGGCTAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  7:149838/136‑1 (MQ=255)                                                                                                                        tttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGGGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  7:75564/136‑1 (MQ=255)                                                                                                                         ttCCCGCCGCCGCGGCGGCGAGTTTGCCGGGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  1:139843/135‑1 (MQ=255)                                                                                                                         ttCCCGCCGCCGCGGCGGCGAGTTTGCAGGGCTGGCACCGGGCATGGGGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  6:243467/135‑1 (MQ=255)                                                                                                                         ttCCCGCAGCCGCGGCGGCGTGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  4:4528/135‑1 (MQ=255)                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  2:226775/135‑1 (MQ=255)                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGCAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  3:245977/135‑1 (MQ=255)                                                                                                                          tCCCGCAGCCGCGGCGGCGAGTTTGCCGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCTGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt  <  5:125481/134‑1 (MQ=255)                                                                                                                             |                                                                                                                                   TCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGT  >  KT2440/179360‑179614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑