| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,757,234 | Δ1 bp | 100% | coding (776/834 nt) | CJ019_05400 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,757,234 | 0 | A | . | 85.6% | 141.0 / 9.6 | 41 | coding (776/834 nt) | CJ019_05400 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (30/5); minor base T (0/3); total (30/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCA > KT2440/5757114‑5757354 | gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCca > 7:156064/1‑133 (MQ=255) gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagca > 7:325603/1‑134 (MQ=255) gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCaagaa > 1:354516/1‑132 (MQ=255) aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagcag > 1:88436/1‑133 (MQ=255) aCCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAgcgc > 2:281970/1‑135 (MQ=255) gACCTGGCCAAGGTCTTGAAGACCATCGAGGAACCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg > 3:297178/1‑136 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGccgccgccg > 1:241795/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc > 3:265846/1‑136 (MQ=255) tGGCCAAGGTCTTGAAGACCATCGAGGAAACACTCTCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCACGAAGGCCCTCCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCCGCAGGAAGCGCTGGccgccgccg > 1:174099/1‑135 (MQ=255) tGGCCAAGGTCTTGAAGACCATAGAGGAAACACTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGACGCCAGCAGGAAGCGCTGgccgccgcc > 2:32348/1‑134 (MQ=255) aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc > 4:172020/1‑136 (MQ=255) aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc > 3:269284/1‑136 (MQ=255) cTTGAAGCCCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCTGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc > 4:40076/1‑136 (MQ=255) aacaCCATCGAGGAACCCCTCGCCCGCCAGGCCCGTCAAGCTCAAGAGGCACGCAAGACGGCCCTGCTGCCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc < 7:47667/129‑1 (MQ=255) aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg > 8:226618/1‑135 (MQ=255) cATCGAGTAAACCCTCGCCGGCCAGGCCCGTGAAGCTGAAGCGGCACGCAATAAGGCCCTGCTGGCGCAGCAGTAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg < 8:307334/135‑1 (MQ=255) tgcGAGAAAACCAGCGCCGGCATGGCCGTGGACGCTGACGAGGCAGGCACGATGGCCCTGCTGGCGCAGCTGTAAG‑GGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGt < 2:354516/133‑1 (MQ=255) cggAAACCCTCGCCCGCCCGGCCCGTGAAGCTGACGAGGCCCGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc < 8:298788/134‑1 (MQ=255) agcacaCCCTCGCCCGCCGCGCCCGGTACGATCAAGAGGCCCGCAACACGGCCCGGCGGGCCCCGAAGAACGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc < 6:33849/130‑1 (MQ=255) aGGACACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc > 1:46167/1‑135 (MQ=255) aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc > 8:63950/1‑135 (MQ=255) aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc > 1:22273/1‑135 (MQ=255) aGGAAACCCTCGCCCGCAAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGCAAGCGCTGTCCGCCGCCGCTGCCCGCGACCGGCACCGTGAg > 2:340257/1‑134 (MQ=255) aacccccGCGCCCGCCAGCCCGGGGACGGTCCATGGGCAGCCAACAAGCCCGTCCTGGCGCAGC‑TGTATCCGCAAAAACGCCGCCAGCTGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt < 2:48515/131‑1 (MQ=255) ccccCGCCCGCCCGGCCCGGGAAGCGGACGCGGCCGGCACGACGGCCCTGCTGGCGCAGCAGGACGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCag < 6:53918/135‑1 (MQ=255) cccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAg > 1:54019/1‑135 (MQ=255) ccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGt > 5:309249/1‑135 (MQ=255) ggCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAcc > 8:229707/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCTCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa > 2:284683/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGCGCCTGCAGCGCCAGTGGAACCGCCGaaaaa > 8:180747/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa > 6:74049/1‑135 (MQ=255) aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGAAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGACAGTGGAACCGCCGAAAAAg > 4:300397/1‑136 (MQ=255) cTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg > 7:122883/1‑135 (MQ=255) acagGCACGCAAGACGGCCCTGCTGGCGCAGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg < 7:26040/133‑1 (MQ=255) gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg > 4:279929/1‑136 (MQ=255) aagGACCTGCTGGCGCAGCAGGAAGCTG‑AAAAACTCCTCCAGCAGTAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTTAGCCTGCAGAGCCAGTGGAACCGCAGAAAAAGGCCCGTACCACCCTCGGGc < 4:92059/136‑1 (MQ=255) gCCCGGCGGGCCCAGCCGGAAG‑GGTAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCg < 2:88436/135‑1 (MQ=255) gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt > 6:77089/1‑135 (MQ=255) tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAACAAGGCCCGTACCACCCTCGGGCCGATGGttt > 2:17584/1‑135 (MQ=255) gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCCCCCTCGGGCCGATGGttt > 7:56699/1‑133 (MQ=255) gcgcAGCAGGAAGC‑GCAAAACCGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCa < 4:265846/136‑1 (MQ=255) | GCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCA > KT2440/5757114‑5757354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |