Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,357,456 C→A 8.6% L366M (CTG→ATG)  dctA2_1 → C4‑dicarboxylate transport protein 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,357,4560CA 8.6% 126.8 / 4.7 47L366M (CTG→ATG) dctA2_1C4‑dicarboxylate transport protein 2
Reads supporting (aligned to +/- strand):  ref base C (28/15);  new base A (2/2);  total (30/17)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

CCACTGACACCACCATGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGCCAAGTGGGTCAAGGAAATGGACAA  >  KT2440/1357322‑1357586
                                                                                                                                      |                                                                                                                                  
ccACTGACACCACCATGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCCCCt                                                                                                                                   >  3:84302/1‑136 (MQ=255)
       caccacCATGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGt                                                                                                                             >  1:281264/1‑135 (MQ=255)
            ccaTGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCg                                                                                                                        >  8:124057/1‑135 (MQ=255)
               tGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGcc                                                                                                                     >  8:124699/1‑135 (MQ=255)
               tGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCt                                                                                                                    >  4:103042/1‑136 (MQ=255)
                       actcacCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCGTCATCGTGCTGGCCGCGCCCCTGTCGGCTGTTTGCCCCCTGCCGGTTGGCGGCCTGGCGCt                                                                                                              >  3:61577/1‑134 (MQ=255)
                              aGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGGCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCCCCTGCCGGGTGCCGGCCTGGCGCTGAGCCTccg                                                                                                     >  4:310616/1‑134 (MQ=255)
                              aGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCGGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATACTCg                                                                                                      >  6:20385/1‑135 (MQ=255)
                              aGATCACCCTGCTGCTGGTGCGGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGGCTGGCGCTGCTCCTCg                                                                                                      >  8:299538/1‑135 (MQ=255)
                                  cACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATc                                                                                                 <  3:286874/136‑1 (MQ=255)
                                   aCCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGGTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCCTc                                                                                                 >  7:51237/1‑135 (MQ=255)
                                   aCCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATc                                                                                                 >  5:54971/1‑135 (MQ=255)
                                   aCCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATc                                                                                                 >  7:60520/1‑135 (MQ=255)
                                   aCCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCAGCCGCTTGGCGGCCGGGCGCTGATCCTCGGcca                                                                                                  >  8:118938/1‑132 (MQ=255)
                                   aCCCTGCTGCTGGTGCTGCTGGGGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATc                                                                                                 <  1:308618/135‑1 (MQ=255)
                                         ctgctggtgctgctggtgGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCt                                                                                          <  3:103042/136‑1 (MQ=255)
                                                 gctgctggtgGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTc                                                                                   <  6:54971/135‑1 (MQ=255)
                                                 gctgctggggGCTTCCAAGGGCGCTGCCGGCGTTACCGGGGCGGGCTTCATCGAGCTGCCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTc                                                                                   <  8:285311/135‑1 (MQ=255)
                                                                         tGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCt                                                                                                                                   <  7:372169/63‑1 (MQ=255)
                                                                         tGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCt                                                                                                                                   >  8:372169/1‑63 (MQ=255)
                                                                          gCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATGCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGCCCAACCtg                                                          >  1:193708/1‑135 (MQ=255)
                                                                               cGTTACCGGGGCGGGCTTCAGCGTGCGGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGc                                                    <  4:320443/136‑1 (MQ=255)
                                                                                   aCCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCGGTTGGCCCCCTGCCGGTTGCCGGCCTGGCGCTGCTCCGAGGCATCGCCCGCTTCATGTCCGAAGCCCCCGCCCGGACCAACCGGGTGGGcccc                                                 >  8:280774/1‑132 (MQ=255)
                                                                                        ggcGGGCTTCATCGGGCGGGCCGCGACCCTGTCGGCTGTGGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGt                                            <  1:353906/133‑1 (MQ=255)
                                                                                          cGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTg                                          <  5:144388/135‑1 (MQ=255)
                                                                                            ggCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGCTCCTCGGCATCGCCCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCa                                       >  3:271801/1‑136 (MQ=255)
                                                                                             gCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGATGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACGTGGTGGGCAACGCCGTTGCCa                                       <  7:118938/135‑1 (MQ=255)
                                                                                              cTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTTGCGCTGATACTCGGCATAGACCGCTTCATGTCCGAAGCCCGCGCCCTGCCCAACCGTGTTGGCCCCGCCGTTGcgag                                      >  7:140387/1‑132 (MQ=255)
                                                                                                     gTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGACGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTa                               >  7:3155/1‑135 (MQ=255)
                                                                                                         tGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGCCCGCTTCATGTCCGCAGGCCGCGCCCTGACCCCCCTGGTGGGCACCGCCGTTGCCACCGTGGGAGTgg                           >  6:49188/1‑135 (MQ=255)
                                                                                                         tGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCg                                                                                            >  3:240905/1‑70 (MQ=255)
                                                                                                         tGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCg                                                                                            <  4:240905/70‑1 (MQ=255)
                                                                                                           gCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGcc                         <  5:257120/135‑1 (MQ=255)
                                                                                                           cccGCGACCCTGTCGGGTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGcc                         <  2:170788/134‑1 (MQ=255)
                                                                                                            ccGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGc                          <  2:281264/133‑1 (MQ=255)
                                                                                                              gcgACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGCCCAACCTGGTGGGCAACGCCGTTGCCCCCGTGGTAGTGGCCAAg                      >  7:362489/1‑135 (MQ=255)
                                                                                                              gcgACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGCCAAg                      >  6:356224/1‑135 (MQ=255)
                                                                                                                gACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGCCCGCTTCATGTCCGCAGCCCGCGCCCTGACCCACCTGGTGGGCCCCGCCGTTGCCACCGTGGTAGTGGCCAAGTg                    >  6:63259/1‑135 (MQ=255)
                                                                                                                        cGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGCCAAGTGGGGCAAg             >  8:194101/1‑134 (MQ=255)
                                                                                                                        cGGCAGTTGGCCACCAGCCGGTTTCAGGACTGGCGATCATAATCGGCATCGACCGCTTAAATTAAGAAGACAGCGCACTGACCAACCTGGTGGGCAACTCAGATGCCACCGTGGTAGTGGCCAAGTCGGTCAAg             >  2:238840/1‑134 (MQ=255)
                                                                                                                            tGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGCCAAGTGGGTCAAGGAAAt        <  1:35747/135‑1 (MQ=255)
                                                                                                                               tGGCCACATGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGcc                         <  6:292622/115‑1 (MQ=255)
                                                                                                                               tGGCCACATGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGcc                         <  2:142313/115‑1 (MQ=255)
                                                                                                                               tGGCCACATGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGcc                         >  5:292622/1‑115 (MQ=255)
                                                                                                                               tGGCCACATGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGcc                         >  1:142313/1‑115 (MQ=255)
                                                                                                                                 gCCACCTGCCGGTTGCCGGCCTGGCGGTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGCCAAGTGGGTCAAGGAAATGGACaa  >  3:202187/1‑136 (MQ=255)
                                                                                                                                 gCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCACCGCCGTTGCCACCGTGGTAGTGGCCAAGTGGGTCAAGGAAATGGACaa  >  3:18597/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CCACTGACACCACCATGGACATCACTCACCAGATCACCCTGCTGCTGGTGCTGCTGGTGGCTTCCAAGGGGGCTGCCGGCGTTACCGGGTCGGGCTTCATCGTGCTGGCCGCGACCCTGTCGGCTGTTGGCCACCTGCCGGTTGCCGGCCTGGCGCTGATCCTCGGCATCGACCGCTTCATGTCCGAAGCCCGCGCCCTGACCAACCTGGTGGGCAACGCCGTTGCCACCGTGGTAGTGGCCAAGTGGGTCAAGGAAATGGACAA  >  KT2440/1357322‑1357586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑