Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,508,681	T→G	18.1%	V187G (GTG→GGG)	ftsI →	Peptidoglycan D,D‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,508,681	0	T	G	18.1%	77.9 / 2.3	49	V187G (GTG→GGG)	ftsI	Peptidoglycan D,D‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base T (27/10);  new base G (4/6);  total (33/16)
Fisher's exact test for biased strand distribution p-value = 6.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTACCTGGCTACCCGC  >  KT2440/1508553‑1508814                                                                                                                                    |                                                                                                                                      cGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAATGGGGGGGGCGGGCCTATGAAGCGTGGctggccg‑g‑cctggccggc                                                                                                                                >  4:705/1‑136 (MQ=255)  ggACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGAGCGGGGCGATGAAGAGTGGCTTGGCC‑G‑CGTGccccg                                                                                                                                 >  8:781/1‑132 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GTACGGGTGGCGCAGGCCGATTAAGAGTGGCTGGCCG‑GCCGTGCCC‑GCAAgcgc                                                                                                                          >  3:284731/1‑136 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GGAGGGGTGGAGCTGGCCGATGAATAGTTGCTGGCCG‑G‑CGTGCCCGGCAAgcg                                                                                                                           >  5:79207/1‑135 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GCAGGGGTGGGGCTGGCCGATGAAGCGTGGCTGGCCG‑G‑CGTGCCCCGCAAgcg                                                                                                                           >  6:184337/1‑135 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GCAGGGGTGGAGCTGGGCGATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCCAgcg                                                                                                                           >  1:11713/1‑135 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCCGCAAccg                                                                                                                           >  8:239552/1‑135 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAACGGGTGGAGCTGGCCTATGCAGAGTGGCTGGCCG‑G‑CGGGCCCGGCAAgcg                                                                                                                           >  6:178447/1‑135 (MQ=255)       ggAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGACGT‑GCACGGGGGGGGGTGGCCTATGAAGACTGGCGGGGCG‑G‑CGGGCCCGGccaccc                                                                                                                           >  1:115059/1‑130 (MQ=255)       ggAAGAATTCCGCCGCTTCAATCCGGCCGGTGATGTGAATGCGCACATGGTCCGCTTTACCGACCTCGACGACCACCGACGTCGATGGGGT‑GCGCTCGCCAATGAAAACTTGCTGGGCGCG‑CG‑GCCCCGCAAgac                                                                                                                           >  2:287533/1‑133 (MQ=255)                 cgccgcTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GGAGGGGTGGCGCTGGGCGATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCCGGTgct                                                                                                                  >  2:88481/1‑132 (MQ=255)                      cTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAACGGGTGGCGGCGGGCGATGGAGAGTTGCTGGCCC‑G‑CGGTCCCGGGCAACGCCCCGTGATCAAAGa                                                                                                            >  6:5625/1‑135 (MQ=255)                         cTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGGGCTGGGCGATGAAGTGTTGCTGGGCG‑G‑CGGGCCCGGCAAGCGCACGGTGCTCAAGGCCCg                                                                                                         >  2:303660/1‑135 (MQ=255)                                    gTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGAGCTGGCCGATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCCAGCGCCCGGGGATCAATGACCGGCgcgggcggg                                                                                              >  2:292750/1‑134 (MQ=255)                                    gTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGGGGCGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGc                                                                                              >  8:351690/1‑135 (MQ=255)                                    gTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCCT‑GAAGGCGGGGACCTGGCCGATGAAGAATGGCTGGCCG‑G‑CGAGCCCCGCAAACGCCAAGTGATCCAGGACCGGCGCggagggc                                                                                              >  8:297075/1‑130 (MQ=255)                                          tgccggcgcACAGGGGCGGCTTTACCGCCCGCGACGACCACGGTCGT‑GAAGGGGGGGAGCTGGCCTATGAAGCGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATc                                                                                         <  1:66177/129‑1 (MQ=255)                                                   acaTGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GTAGGGGTGGAGCTGGCCGATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAACGCCAGGTGATCAAGGCCCGGCGCGGTCGGCTGATCAAGGActg                                                                                 >  7:22503/1‑131 (MQ=255)                                                   acaTGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GCAGGGGTGGAGCTGGCCGATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCCAGCGCCAGGTGCTCAAGGACCGGCGCGGGCGGCGGGTCAAGGACAt                                                                                 >  8:311705/1‑133 (MQ=255)                                                              tttACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAACCGCCAGGTGATCAAGGACCGGCGCGGGCGGCGGCTCAAGGACCTTCAGGGAACCaa                                                                     >  1:4377/1‑134 (MQ=255)                                                                     cccTCGACGCCCCGGGGGGT‑GATGGGGGGGTGCTGGCCTAGGACGCGGGGCGGGCGG‑G‑CGGGCCCGGCAAGCGCCAGGTGAGCAAGGACGGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCa                                                             <  7:16126/134‑1 (MQ=255)                                                                           ccgacCCCGGGCGT‑GACGGGGGGGCGCGGCCCTAGGAAGAGGGGCTGGCGG‑G‑GGGGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCg                                                       <  5:107666/134‑1 (MQ=255)                                                                           actacCACGGTCGT‑GAAGGGGTGTAGCTGGCCTAGTACGCGTGGGTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGAGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCg                                                       <  7:239552/132‑1 (MQ=255)                                                                           acgacCACGGGCGT‑GAAGGGGTGGAGCGGGCCTATGAAGAGTGGCGGGCCG‑G‑CGGGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCg                                                       <  6:146370/135‑1 (MQ=255)                                                                                     gggT‑GATGGGGGGGCGCTGGCATATCACGAGGGGCGGGCGG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCtt                                             <  6:79207/133‑1 (MQ=255)                                                                                      cGT‑GACGGGGGGGCGCGGGCCTAGCAAGCGGGGCGGCCCG‑G‑GGGGCCCGGCACGCGCCAGGTGAGCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTg                                            <  2:230168/135‑1 (MQ=255)                                                                                      cGT‑GACGGGGGGGCGCGGGCCTACGAAGCGTGGCGGCCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTg                                            <  5:87490/135‑1 (MQ=255)                                                                                        t‑GATGGGGTGGAGCTGGCCTATGAAGAGGGGGGGGCGG‑G‑GGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc                                          <  6:163857/135‑1 (MQ=255)                                                                                        t‑GAGGGGGGGGAGCGGGCCTAGCCCGGGGGGCGGGCGG‑G‑GGGGCCCGCCACGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc                                          <  1:141242/135‑1 (MQ=255)                                                                                        t‑GACGGGGTGTAGCGGGCCTATGAAGAGTGGCGGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACAATCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc                                          <  6:49038/135‑1 (MQ=255)                                                                                        t‑GAAGGGGTGGAGCGGGCCTATGAAGCGGGGCGGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGc                                          <  2:11713/135‑1 (MQ=255)                                                                                                   gAGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGaa                                 >  8:134840/1‑133 (MQ=255)                                                                                                   gAGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGACGGCTGATCAAGGACATACAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGa                                  >  4:240816/1‑133 (MQ=255)                                                                                                     gCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAgcc                             >  3:243082/1‑134 (MQ=255)                                                                                                      cgggCCTATGAAGAGGGGCGGGCCG‑G‑CGGGCCCGGCAAGCGCCAGGTGATCACGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGAcc                            <  3:317162/134‑1 (MQ=255)                                                                                                      cTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGAc                             >  1:30055/1‑135 (MQ=255)                                                                                                           cTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTgcg                        >  7:256344/1‑135 (MQ=255)                                                                                                            tATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTgcgc                       >  8:53426/1‑135 (MQ=255)                                                                                                                 agagTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCa                  >  1:328341/1‑135 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGAGATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTACCt            >  3:271797/1‑136 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAAcc                                                                       >  7:213670/1‑77 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAAcc                                                                       <  8:213670/77‑1 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTAcc             >  8:276689/1‑135 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTAcc             >  2:134163/1‑135 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTACCt            >  4:206967/1‑136 (MQ=255)                                                                                                                      ggctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCGGCGCCTGCAGTAcc             >  2:133479/1‑135 (MQ=255)                                                                                                                      ggcgggccg‑g‑cgggccCGGAACGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTAcc             <  5:184337/135‑1 (MQ=255)                                                                                                                      ggcgggcCG‑G‑CGTGCCCGCCAAGCGCCAGGTGAGCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTAcc             <  5:178447/135‑1 (MQ=255)                                                                                                                        ctggcCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTACCTg           >  7:204963/1‑135 (MQ=255)                                                                                                                                  cGTGCCCGGCAAGCGCAAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGGGTTGTCGATAGACCTGCGCCTGCAGTACCTGGCTACCcg   >  7:208311/1‑135 (MQ=255)                                                                                                                                   gTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTACCTGGCTACCcgc  <  7:351690/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      CGGACTGGAAGAATTCCGCCGCTTCTATCCGGCCGGTGATGTGACTGCGCACATGGTCGGCTTTACCGACCTCGACGACCACGGTCGT‑GAAGGGGTGGAGCTGGCCTATGAAGAGTGGCTGGCCG‑G‑CGTGCCCGGCAAGCGCCAGGTGATCAAGGACCGGCGCGGTCGGCTGATCAAGGACATTCAGGTAACCAAGAACGCCAAGGCCGGGAAGACCTTGGCGTTGTCGATAGACCTGCGCCTGCAGTACCTGGCTACCCGC  >  KT2440/1508553‑1508814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑