Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,528,308 A→C 38.4% P87P (CCA→CCC)  argJ → Arginine biosynthesis bifunctional protein ArgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,528,3080AC38.4% 27.1 / 6.7 44P87P (CCA→CCC) argJArginine biosynthesis bifunctional protein ArgJ
Reads supporting (aligned to +/- strand):  ref base A (20/5);  new base C (16/2);  total (36/8)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCT  >  KT2440/1528177‑1528439
                                                                                                                                    |                                                                                                                                    
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGc                                                                                                                                   >  1:12531/1‑135 (MQ=255)
aGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCGTCCTGCTGCCCACCCCGGG‑TAACGCCCACGCCGGCCCCGGCGCCCCCGGc                                                                                                                                   >  6:248505/1‑135 (MQ=255)
         ggcGTGTTCCCCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCCCCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCCCCGGCGCGCCCGGCCGGgccgcc                                                                                                                          >  5:44812/1‑135 (MQ=255)
                  aCCCCCACCGCCTTGGGCGCGGCGCCGGGTGTCCTGCCCAAACCGGGTGTACCGGCAACGGTGCGCTACCTGCGCACAAACCCGGG‑AACCGCCACCGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAgcg                                                                                                                  <  6:158157/134‑1 (MQ=255)
                           gCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTgcgc                                                                                                         >  2:17193/1‑134 (MQ=255)
                                 tGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCGGCGCCAAGcag                                                                                                  >  6:279322/1‑133 (MQ=255)
                                 tGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTg                                                                                                  >  1:35285/1‑135 (MQ=255)
                                 tGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCACGCCTGGCCGCCGCCGAGCGCACCGGCGCCAAGCTgg                                                                                                 >  3:130595/1‑136 (MQ=255)
                                   cgcgGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCGGCGCCAAGCTGGc                                                                                                >  8:312251/1‑135 (MQ=255)
                                        cgcCGGGGCTCCTGCCCAACCCCCGTGACCAGGGCACGTGGGGCGACC‑GCCGCCCCACCCGGGTTACCGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAAc                                                                                           <  8:182565/135‑1 (MQ=255)
                                           cGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCAGGCCGCCGCCGAGCGCACCTGCGCCCCGCTGGCCGAActgg                                                                                        >  6:196778/1‑135 (MQ=255)
                                           cGGTGATCCTGACCAACCCGCGTTTACCGGGCACGGGGCGCTACCTGCTGACCACCAGGGG‑AAACCCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAActgg                                                                                        <  6:139192/135‑1 (MQ=255)
                                                      cccAAACCGCGTGTCCTGGGCACGGTGGGTTACCTTGTGACCACCAGGGG‑TAACGCACACGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTAcc                                                                              <  6:62148/133‑1 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTa                                                                 >  1:283950/1‑135 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTAc                                                                >  4:122133/1‑136 (MQ=255)
                                                                  gtACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCCCGCCCGGCCAGGCCGCCCCCGAGCGCACCTGCGCCCAGCAGGCCGAACAGGCAGGCGTACCGGCCGACTCggg                                                                  >  2:110913/1‑133 (MQ=255)
                                                                   tACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACt                                                               >  4:320464/1‑136 (MQ=255)
                                                                       gggCACCGGGCGCAACCCCCGCACCACCCCGGG‑TAACGCCCCCGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGcc                                                            <  7:337461/135‑1 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGCGTCGGTACTGCCGtt                                                         >  2:61129/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCCAGCTGGCCGAACTGGCTGGCGTACCGGCCGCGTCGGGACTGCCCtt                                                         >  1:156420/1‑135 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTCCTGCCGTtc                                                        >  4:200862/1‑136 (MQ=255)
                                                                          cACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCAGGCCGCCGCCGCGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCggt                                                         >  5:263824/1‑133 (MQ=255)
                                                                             cGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCAGGCCGCACTGGCTGGCGTTCCGGCCGAGTCGGTACTGCCGGTCT‑cc                                                    >  4:118434/1‑136 (MQ=255)
                                                                             cGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCAGCGCCACGCTGGCCGAACTGTCTGGCGTACCGGCCGAGGCGGTACAGCCGTtct‑c                                                     >  1:129506/1‑135 (MQ=255)
                                                                              gTGCGCTACCTGCTGACCACCACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCCCGCGCACCTGCGCCAAGCGGGCCGACCTGGCTGGCGTCCCGGCCGCGGGGGTCCTGCCGGTCT‑cc                                                    >  7:157601/1‑135 (MQ=255)
                                                                              gTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCCCGCGCACCTGCGCCCAGCAGGCCGCACGGGCTGGCGTACCGGCCGCGTCGGGACTGCCGGTCT‑cc                                                    >  1:181905/1‑135 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGCACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑cccc                                                  >  6:206734/1‑133 (MQ=255)
                                                                                gcgcTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCAc                                                  >  2:105455/1‑135 (MQ=255)
                                                                                     aCCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCAGGCCGCCGCCCCGCGCCCCAGCGCCCAGCTGGCCGCACTGGCTGGCGTACCGGCCGCCTCCGGACGGCCCTGGT‑CCCCCGGCg                                             >  5:234066/1‑135 (MQ=255)
                                                                                     aCCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCCCCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCg                                             >  7:192905/1‑135 (MQ=255)
                                                                                     aCCTGCTGACCAACACCGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGACAGGCCCCCGCCCAGCGCACCGGCGCCAAGCAGGCCGAACAGGCTGGCGTACCCGGCCAGTCGGTACAGACGTTCT‑ACCCCga                                               >  2:112501/1‑132 (MQ=255)
                                                                                             accaccCCGGG‑CACCGCCAACGCCGGCCCCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGTGATTGGc                                     <  6:44812/135‑1 (MQ=255)
                                                                                                aaCACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCCAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGGGGTTGGCGAg                                  >  7:264695/1‑135 (MQ=255)
                                                                                                     ggg‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGTGCTTGGCGAGCCGca                             >  5:140966/1‑134 (MQ=255)
                                                                                                       g‑TAACGCCAACGCCGGCACCGGCGCGCCCGGCCTGGCCGCCGCCCAGCGCCCCTGCGCCCAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCCGCGTGGTTTGGGAGccccggc                           >  6:201480/1‑130 (MQ=255)
                                                                                                           acgccaacgccGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGTGATTTGCGATCCccggccgg                        >  8:206579/1‑135 (MQ=255)
                                                                                                             gccaacgcgGCCCCCGGCGCGCCCGGCCTGGCGGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGTGATTGGCGAGCCGCTGCCGGTc                      <  6:199805/135‑1 (MQ=255)
                                                                                                                     cGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGCGCGCCCCTGCGCCAAGCTGGCCGACCTGGCTGGCGTACCGGCCGCGGCGGTACTGCCGGTCT‑CCACCGGCGGGGTTGGGGGGCCGCGGCCGGGCGGAAAGAtg             >  3:331863/1‑135 (MQ=255)
                                                                                                                     cGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGTCTGGCGTACCGGCCGAGGCGGTACTGCCGTTCT‑CCACCGGCGTGATTGGCGAGCCGCGGCCGGGGGAAAAgct              >  2:327070/1‑133 (MQ=255)
                                                                                                                     cGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCTCCCCCCGGCG‑GATTGGCGAGCCGCGGCCCGGGGaaaacta              >  6:34149/1‑132 (MQ=255)
                                                                                                                          ccGGCGCGCCTGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGGCGGTACTGCCGGTCT‑CCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAg         <  1:110913/135‑1 (MQ=255)
                                                                                                                                cgcCCGGCCTGGCCGCCGCCGCGCGCCCCGGCGCCCAGCTGGCCGAACTGGCTGGCGTACCGGCCGCGGCGGGACTGCCGTTCT‑CCCCCCGCGTGGTTTGCGGGGCGCGGCCCGGGGCAAAGATGGGAGgcgcgc   >  2:217650/1‑135 (MQ=255)
                                                                                                                                cgcCCGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGGGATTGGCGCGCCGCGGCCGGGCGAAAAGATCGAAGGCGCGCt  >  4:114664/1‑136 (MQ=255)
                                                                                                                                cgcCAGGCCTGGCCGCCGCCCAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGGTCT‑CCACCGGCGTGAGTGGGGCTCCGCGGCCCGTCCGAAAGATCTCACgcgcgc   >  2:236980/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                    
AGCGTGGCTGGCGTGTTCACCCTCAACGCCTTCTGCGCGGCGCCGGTGATCCTGTCCAAACAGCGTGTACAGGGCACCGTGCGCTACCTGCTGACCAACACGGG‑TAACGCCAACGCCGGCACCGGCGCGCCAGGCCTGGCCGCCGCCGAGCGCACCTGCGCCAAGCTGGCCGAACTGGCTGGCGTACCGGCCGAGTCGGTACTGCCGTTCT‑CCACCGGCGTGATTGGCGAGCCGCTGCCGGTCGAAAAGATCGAAGGCGCGCT  >  KT2440/1528177‑1528439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑