Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,077,164	T→G	35.6%	L173R (CTG→CGG)	artJ_2 →	ABC transporter arginine‑binding protein 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,077,164	0	T	G	35.6%	21.9 / 5.1	40	L173R (CTG→CGG)	artJ_2	ABC transporter arginine‑binding protein 1
Reads supporting (aligned to +/- strand):  ref base T (20/2);  new base G (10/2);  total (36/4)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACGAAG  >  KT2440/4077044‑4077298                                                                                                                          |                                                                                                                                        gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTg                                                                                                                           >  8:363344/1‑135 (MQ=255) gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGc                                                                                                                          >  4:279987/1‑136 (MQ=255) gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCGGGACGGCGTGCTTg                                                                                                                           >  8:209512/1‑135 (MQ=255)  tGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGcc                                                                                                                         >  4:256584/1‑136 (MQ=255)    cgcAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACTGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGCCCTGGCGGCC‑GGCCGCCTGGCCGGCGGGCGTGCCg                                                                                                                        >  8:180156/1‑135 (MQ=255)         tCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGACAAAt                                                                                                                  >  4:167842/1‑136 (MQ=255)                 tACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCCCCCGGGCCGGGGGGTTGGCGCCAAATTaccccg                                                                                                           >  2:356143/1‑132 (MQ=255)                    ccAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCa                                                                                                        >  8:329758/1‑135 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGCCGGCGTGAATGCCGACAAATTCCCGCTGCa                                                                                                        >  8:119713/1‑133 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGCCCAATTCCCGCTGCa                                                                                                        >  7:297499/1‑133 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCCGGCCCGCGGGCTTGGCGCCAAATTACCGCCGCACg                                                                                                      >  2:361114/1‑135 (MQ=255)                      aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGAGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCCCCAGGACGGCGTGATTGCCGACAAATTCCCCCTGCa                                                                                                        >  8:328180/1‑133 (MQ=255)                        gCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCACCGCAGACCTGGCGGCC‑GGGCGCCGGGCCCGCGGGCTTTCCGGCAAATTACCGCGGCagg                                                                                                      >  7:152297/1‑131 (MQ=255)                        gCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGCCGGCGGGGATGCCGCCCAAATACCCCGGCACgcg                                                                                                    >  2:304453/1‑133 (MQ=255)                         caaTCAACCCCGAAGGCGGGACCGCCAAGGCCGGGGCCTAGGTCAAATTAACCCCGACGCTGCACTAAGCCACCCCAGACCTGGGGGCC‑GGCGGCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGt                                                                                                   <  6:334188/133‑1 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGCCCTGGCGGCC‑GGCCGCCGGGACGGCGTGATTGCCGACAAAATCCCGCTGCACGAGTgg                                                                                                 >  7:305273/1‑135 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCGGGACGGCGTGATTTCCGCCAAATTACCGCTGCCCGCGTggc                                                                                                >  3:32178/1‑135 (MQ=255)                           aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCCCCAGGCCGGCGGGGTTGCCGACAAATTACCGCTGCACGAGTgg                                                                                                 >  7:37223/1‑135 (MQ=255)                           aTCTACGCCAAAGACGTGTACGGCAAGGCCGGTGACGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTCGACGGCGTGATTGCCGAGAACTTCCCTCTGCACGAGTGGa                                                                                                >  4:298593/1‑136 (MQ=255)                                     aaGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑Gaa                                                                                     >  4:305875/1‑136 (MQ=255)                                              aCGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGGCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCgggg                                                                             >  8:209990/1‑135 (MQ=255)                                                ggCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGGCCGCGGGGCCGCCGGGTTGGCCACCAAATACCC‑CTCCCCGAGGGGTGGAACAA‑CAACGGCgggggt                                                                           >  2:168955/1‑133 (MQ=255)                                                ggCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGGCGGCGGGATTGGCCACAAATTCCCGCTGCAGGACTGGCTGTAAAA‑GAAGCGCCGGGAt                                                                           >  1:241520/1‑135 (MQ=255)                                                ggCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCCCCCGGGCGGGGGGCTTGCCGCCCAAATTCCGCGGCCCGCGGGGATGGACAACCAACGggggggt                                                                            >  5:351888/1‑134 (MQ=255)                                                ggCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCGGGCGGCC‑GGCCGCCGGGGCGGCGGGGTTGCCGCCAAATTACCGCGGCCCGAGTGGATGGACAA‑GAACGGCGGGGCtt                                                                          >  4:359674/1‑136 (MQ=255)                                                       ccGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCGGGGCGGCGGGGTTGGCGACAAATTCCCGCGGGCCGCGTGGTTGAACAA‑GAACGGCGGGGGTtgggg                                                                      >  1:76893/1‑130 (MQ=255)                                                        cGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATtgctg                                                                      >  6:59507/1‑132 (MQ=255)                                                        cGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCGGGCCGGCGTGATTGCCGCCCAATTACCGCGGCACGGGGGGCTGAACAA‑GGACGGCGGGGGTTGCTGCaa                                                                   >  5:108168/1‑135 (MQ=255)                                                                   tGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑CGCCGCCAGGGAGGCGTTATTTCCGCCAAATTACCCCTGCACCAGTGGATGAAAAA‑GAACGGCGGGGCTTGCAGGAACATACTTGGCg                                                        >  6:76712/1‑135 (MQ=255)                                                                        ctgaccccGCCCATGGACGAAGCCAACGCAGCCCTGGCGGCC‑GGCCGCCGGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTg                                                   <  8:305273/130‑1 (MQ=255)                                                                              ccGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGCCGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCGGCAAGATCCTTGGCGCCGTGGCgggc                                             >  6:131892/1‑133 (MQ=255)                                                                                        aCGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGGGATTGCCGACAAAATACCCCTGCCCGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTTGCGACGTGGCGGACACCAAGGCCg                                   >  1:299603/1‑135 (MQ=255)                                                                                        aCGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCGGGACGGCGGGATTGGCGCCAAATTACCGCGGCCCGAGGGGATGAACAA‑GAACGGCGGGGCTTGCTGCAAGATCCGTGGCGACGTGGCGGCCCCCCAGGcc                                    >  1:206210/1‑134 (MQ=255)                                                                                        aCGAAGCCAACGCAGACCTGGCGGCC‑CGCCGCCTGGACGGCGTGATTGCCGACAAATTACCGCTGCACGAGGGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCg                                   >  8:116464/1‑135 (MQ=255)                                                                                               cAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGGGATTGCCGACAAATTCCCGCTGCCCGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAACGCCGATgccgc                            >  8:246359/1‑135 (MQ=255)                                                                                                          tGGCGGCC‑GGCCGCCGGGCCGGCGGGCTTGCCGCCAAATTCCCGCTGCCCGCGTGGATGAACAA‑GAACGGCGGGGATTGCGGCAAGCTCCTTGGCGACGTGGCGGGCCCCCAGGCCGCTGCCGCCCTCGCGGTgc                 >  2:312765/1‑135 (MQ=255)                                                                                                              gccc‑gcccgcCGGGACGGCGTGATGGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCaa             <  1:119856/133‑1 (MQ=255)                                                                                                                    cccgccTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGgacg        <  8:37223/134‑1 (MQ=255)                                                                                                                       gccTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GCACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGGCACCAAGGCCGATGCCGCCAACGCTGTGCGCAAGgacgcc      >  8:289593/1‑132 (MQ=255)                                                                                                                       gccTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATTCCGCCATCGCTGTGCGCAAGgccgcc      >  8:113337/1‑132 (MQ=255)                                                                                                                          tGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACCCCAAGGCCGATTCCGCCATCGCTGTGCGCAAGGACGACGAAg  >  8:193979/1‑135 (MQ=255)                                                                                                                          |                                                                                                                                        GTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCCGCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAA‑GAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACGAAG  >  KT2440/4077044‑4077298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑