| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,789,443 | A→C | 29.0% | V230G (GTG→GGG) | dat ← | Diaminobutyrate‑‑2‑oxoglutarateaminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,789,443 | 0 | A | C | 29.0% | 55.8 / 4.8 | 62 | V230G (GTG→GGG) | dat | Diaminobutyrate‑‑2‑oxoglutarateaminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (22/20); new base C (15/5); total (37/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCAA > KT2440/4789309‑4789576 | ggATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCa > 8:352247/1‑135 (MQ=255) cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCGCCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTc > 3:98658/1‑136 (MQ=255) cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCACCCCCTGCCCCCCTACGAGGATCCCGGCGGCGGGCGGCGGCCCGCCGC‑ttgc > 4:82987/1‑134 (MQ=255) gacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGC‑gtta > 1:85916/1‑134 (MQ=255) acgaTCAACGCCACGCCGGCCTGCTCGGTAAGGCGCCGCACGCCCTTGAGCCATTCGATGGCGGCCGGAACCACCCCGCCCGCACCCAGCACCACTTGTAGCCTCCCGGCGGCGGGCAGCGGCACGCCGC‑TTTCCg < 4:277458/136‑1 (MQ=255) ccTGCTCGGTAATGCGCCGCACGCCCTGGAGCCATTCGATGTCGCCCGGAACCACCCCGCCCTCACCCTGCCCCACTTGGCGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGtttt < 7:351522/135‑1 (MQ=255) atcccccgcACGCCCTTGCGCAATTCTTGGGCGCCCGGAACCACCCCGCCCCCCCCCTGCACCACTTCGCGCATCACGGCGGCGGGCAGGGCCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 3:322522/131‑1 (MQ=255) aTGCGCCGCACGCCCTTGAGCCATTCCATGGCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 3:170183/136‑1 (MQ=255) aTGCCCCGCACGCCCTGGCGCCATTCGATGGCGGCCGGAACCACCCCCCCCCCCCCCTCCCCCACTTCGCGCAGCCCGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGc < 6:149238/135‑1 (MQ=255) tGCGCCGCACGCCCTTGAGCCATTCGATGGCGGCCGCAACCCCCCCGCCCTCCCCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 5:364409/135‑1 (MQ=255) ggggccgcACGCCATGGACCAATTGTTTGGCGGCGGCAACCCCCCCGCCCAACCCCTGACCAACTCCTCTCAACAGGGGGGCGGCCTGGGTCAGGCGGC‑TTTCCTGATCGAGCAGCAGGTTTTCCAGGTAATGCAg < 4:96692/133‑1 (MQ=255) ggcgcCGCACGCCGTTGCGCCATTGGTTGGGGGCCGGAACCCCCCCGCCCTCCCCCTCCCCCACTGGGTGGACCCCGGGGGCGGGCTGGGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 6:372229/134‑1 (MQ=255) cgccgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGC‑GTTCCGGCTCGAGCAGCAGGGTTTGCAGGTAATGGAgg > 6:120102/1‑135 (MQ=255) gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCC‑tt > 2:24754/1‑135 (MQ=255) gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCGGCACCACTTCGAGGCTCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGCTCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCC‑tt > 1:39135/1‑135 (MQ=255) ccATGCGAGGGCGGCCGGACCCCCCCCCCCCTCACCCGCCACCACTTCGAGGAGCCCGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGcc < 3:287319/136‑1 (MQ=255) ccATGCGAGGCCGGCCGTAACCCCCCCCCCCGCCCCCTCCACCCCTTCGCGGATCCGGGCGGCGGGCAGGGCCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGcc < 3:54014/136‑1 (MQ=255) cATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCCCCGC‑GTTGCGGCTGGAGCCGCAGGGTTTGCAGGTTATGCAGGTTGGGC‑GTGCCCCCGGcc > 5:330720/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGC‑GTTCCGGCGCGAGCAGCAGGGTTTGCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCAcc > 1:257124/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCACGCCGC‑AGTCCGGAGCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCAcc > 6:5367/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCCGCCCGCCGC‑GTTCCGGCTGGAGGAGCAGGGTTTCCCGGTAAATCAGGTTGGCCGTTGCCCCCGcccgccc > 2:148490/1‑131 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGC‑GTTCCGGCTCGAGCCGCAGGGTTTCCAGGGATTGCAGGTTGGCC‑TTGCCCCCGGCCGCACCg > 4:20729/1‑136 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCCCCGC‑GTTCCGGATCGAGCAGCCGGTTTTGCAGGTAATGGAGGTTTGCC‑TTGTCCCCCGCCGCAcc > 5:100809/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCCCCGC‑GGTACGGTAGGAGCCGCAGGGTTTGCAGGTAATGGAGGTTTGCC‑GTTACCCCCGccgcccc > 1:141803/1‑130 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTGCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑GTGACCCCCGCCGCAcc > 2:49901/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACAACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCACGGTTTGCAGGTAATGAAGGTTGGCC‑TTGCCCCCCGCCTCAcc > 8:181199/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCAGCACCACTTCGAGGATCACGGCGGCGGGCACCGGCCCCCCGC‑ATTCCGGCTCGACCAGCACGATTTCACGGTAATTCAGGTTGGCC‑TTGACACCGcccaaccc > 2:206723/1‑130 (MQ=255) gCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGC‑GTTCCGGCTCGAGCCGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCCgg > 7:52940/1‑135 (MQ=255) ggAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGC‑GTTCCGGCTCGAGCAGCAGGGTTTGCAGGGAATGGAGGTTGGCC‑TTGGCCCCGGCCTCACCCCCCccgccg > 7:148126/1‑135 (MQ=255) accaccCCGCCCTCCCCCACCCCCCGTGCGTGCAGCCGGGGGGCGGGCAGCGGCCCGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAAc < 6:360796/135‑1 (MQ=255) cgccccgGCCCTCCCCCGCCACCACTTCGAGGACCCCGGCGGGGGCCTCGGGCCGGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACg < 1:19993/132‑1 (MQ=255) cccGCCCCCCCCCGCCACCACTTCGCGCACCCCGGCGGCGGGCCGCGGCCCCCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCa < 8:52940/135‑1 (MQ=255) cGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑GTTGCGGATCCAGGAGCAGGTTTTGCAGGGTATGCACGTTGGCC‑GTGACCCCCGCCGCCCCGCCCCGGCCCCACGGGCAGc > 5:317792/1‑135 (MQ=255) gCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCACGCCGCGTTTACGGATCGCGAAGCCGTTTTTCCTGGTATTGCAGGTTGGGC‑TTGCCCACGGCCTCACCGCCCAGCCCCACCgggacgc > 8:217042/1‑131 (MQ=255) cccccTGCACCCCTGCTCGGAGCCCGGGGGGGGGCCGCGGCAGGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGGTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGt < 6:335320/133‑1 (MQ=255) cACCCTCCACCACTTCGAGGACCCCGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGt < 7:358541/135‑1 (MQ=255) cACCCGGCCCCACTTCGCGGAGCCCGGCGGCGGGCAGCGGCACGCCGC‑TGTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 4:236449/136‑1 (MQ=255) aCCCTGCACACCTGCGAGTAGCACGGCGGGGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTACTGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 7:352247/135‑1 (MQ=255) aCCCTCCCCCACTTCGAGGCCCACGGGGGGGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 6:100173/135‑1 (MQ=255) cccTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGCACGGGCAGCGGGAGTCgg > 3:144246/1‑135 (MQ=255) cTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGCACGGGCAGCGGGAGTCGTAc > 3:159317/1‑136 (MQ=255) cTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGGAGTCGTa > 2:119521/1‑135 (MQ=255) cTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑GTTCCGGATCGCGCAGCAGGGTTTGCAGGTTATGCAGGTTGGGC‑GTGACCCCGGCCTCACCCCCCCCGCCGCACGGGCCGCCGGAGTCGTa > 5:87804/1‑135 (MQ=255) tGCACCACTTCGAGGATCACGGCGGCGGGCACCCGCACGCCGC‑GGTCCTGATCGAGCATCCGGTTTTGAATGTAATGCAGGTTTTCC‑GTGAACCCCGCCACCCCGCCCACGCCGCACCGGCCGCGGGAGTCGTACg > 4:81772/1‑136 (MQ=255) gCACCACTTCGAGCCTCCCGGCGGGGGGCAGGGGCAGGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 2:37884/135‑1 (MQ=255) caccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 4:66933/134‑1 (MQ=255) caccacTTCGAGGAGCCCGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 4:159317/134‑1 (MQ=255) accacTTCGCGGATCACGGCGGGGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 2:39135/133‑1 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTACGGATCCAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCACACCGCCCAGGCCGAACGGGCAGCGGAAGTCGTACggg > 7:296550/1‑133 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCAGCGGCACCCCGC‑TTTCCCGATAGAGCAGCAGGTTTTCCAGGTAATGCACGTTGGCC‑TTGACCCCGGCCTCAACGCCCACGCCGAACCGGCCGCGGTAGTCGTACggg > 8:254220/1‑133 (MQ=255) cacTTCGAGGATCACGGCGGCGGGCACCGGCCCGCCGC‑TTTCCGGATCGAGCACCACGTTTTCCAGGTAATTCAGGTTGGCC‑ATGACCCCGGCCGCACCCCCCAGGCCGAACGGGCAGCGGTAGTCGTACcggg > 4:45334/1‑133 (MQ=255) actgggAGTATCACGGGGGCGGGCTGCGGCCCGCGGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTaa < 8:296550/130‑1 (MQ=255) tCGAGGATCCCGGCGGCGGGCAGCGGCACCCCCC‑TTTACGGCTCGCCGATCAGGTTTTTCAGGTAATTCAGGTTGGCC‑TTGACCCCGGCCTCAACGCCCACGCCCCACGGGCAGCGCTAGTAGTACGGGGAAGGc > 8:64971/1‑135 (MQ=255) tCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGC‑TTTCCGGATCGCGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCC‑TTGCCCCCCGCCACACCGCCCCGGCCGCACCGGCAGCGGGAGTCGTACGGGTAAGGc > 2:105104/1‑135 (MQ=255) tCGAGGATCACGGCGGCGGGCAGCCGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGCCCCCGGCCTCACCGCCCAGGCCGCACCGGCAGCGGTAGTCGTACGGGTAAGGc > 8:30006/1‑135 (MQ=255) cGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt > 3:348067/1‑136 (MQ=255) aTCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAAc > 1:264767/1‑135 (MQ=255) ccACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACt < 7:66980/134‑1 (MQ=255) aCGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGCACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGc > 6:65970/1‑135 (MQ=255) cggcggcggGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGCACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCa > 1:240574/1‑135 (MQ=255) cggcggGCAGCGGCACGCCGC‑TTTGCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGc > 7:91741/1‑135 (MQ=255) ggcggGCAGCGGCCCGCCGC‑TTTCCGGATCGAGCAGCAGGTGTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCg < 4:239418/136‑1 (MQ=255) cACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCCCCGCCCAGGCCGACCGGGCAGGGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGCGCaa > 6:194857/1‑135 (MQ=255) | GGATCTCGTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGC‑TTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCC‑TTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCAA > KT2440/4789309‑4789576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |