Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,817,421	A→C	27.9%	L2531R (CTG→CGG)	lgrB_2 ←	Linear gramicidin synthase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,817,421	0	A	C	27.9%	72.5 / 3.4	57	L2531R (CTG→CGG)	lgrB_2	Linear gramicidin synthase subunit B
Reads supporting (aligned to +/- strand):  ref base A (27/12);  new base C (12/6);  total (39/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTCCACCAGGCCATCGGTACCCAGGCGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGCAAGGCC  >  KT2440/4817289‑4817555                                                                                                                                      |                                                                                                                                       tACTCCACCAGGCCATCGGTACCCAGGCGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCGGGG‑CCacg                                                                                                                                      >  6:64145/1‑133 (MQ=255)        ccaGGCCATCGGTACCCAGGCGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGccc                                                                                                                               >  7:65330/1‑133 (MQ=255)        ccaGGCCATCGGTACCCAGGCGTACCAGCTCGCCGGTCCGGTACAGGAGCCCGCCTTCGGCAATGACCCGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGGAGCCCAGGG‑CCCCGCCGGcccc                                                                                                                              >  3:15624/1‑136 (MQ=255)           ggCCATCGGTACCCAGGCGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGCACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGGCGTGGGTACCCAGGG‑CCCGGCCCGcccccc                                                                                                                            >  6:26533/1‑133 (MQ=38)                 cggacccccGGCGTCCCCGGGCGCCGGTCCGGTCCCGGCGCCCGCCGGCGCCCCTCACCGGGGCGCCGACAACGCGCGCGGCCGGGCGGCCGGGCCGGGCGGGGAACCCCGGGG‑CCCGGCCGGCACCGCCGACGTa                                                                                                                     <  4:23712/131‑1 (MQ=255)                   gTACCCAGGCGTACCAGGTCGCCGGTACGGTACCGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGGCGTGGGACCCCGGGG‑CCAGGCCGGCAccgcccccgg                                                                                                                      >  7:285911/1‑128 (MQ=255)                   gTACCCAGGCGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGCCAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGGAACCCAGGG‑CCAGGCCCGCACCGCCGCCGt                                                                                                                      >  2:262937/1‑133 (MQ=255)                        cAGGCGTCCCAGGGCGCGGGTACGGTACCGGCGCCCGCCTGCGGCACTGACCGGGGCGGCGACAAAGCGCGCGGCAGGGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTc                                                                                                               <  2:28126/135‑1 (MQ=255)                            cGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTACCCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCa                                                                                                           >  2:200785/1‑135 (MQ=255)                              tACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGCCGGACAGGTCGCCAATa                                                                                                        >  4:296485/1‑136 (MQ=255)                                 cAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTGACCCAGGG‑CCCCGCCCGCCCCGCCCCCCGACAGTTCGCCCAAAcc                                                                                                      >  2:151000/1‑135 (MQ=255)                                        ccGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGGAACCCTGGG‑CCCGGCCGGCACCGCCGCCGTACAGTTCGCCAATACCGCCTTGc                                                                                               >  6:305866/1‑135 (MQ=255)                                                         cccgccTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCGGGG‑CCCGGCCCGCCCCGCCGACGGACAGTTCGCCAATACCGCCGTGCGGCAACAACGCCAGAt                                                                               >  2:282/1‑134 (MQ=38)                                                           cgccTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCCCCGCCGCCGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGctcctc                                                                           >  3:82870/1‑136 (MQ=255)                                                            gccTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCCGGCCGGCACCGCCGCCGTACACTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCtc                                                                           >  8:299839/1‑135 (MQ=255)                                                             ccTGCGGCACTGACCGGGTCGGCCACAAAGGGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCtcg                                                                          <  2:49397/135‑1 (MQ=255)                                                             ccTGCGGCACTCCCCGGGGCGCCCAAAAAGCGCTCGGCTGGGAGGCCTGGCCGTGCGGGGAAACCCGGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCtcg                                                                          <  5:256617/135‑1 (MQ=255)                                                              cTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTATCCCGGGG‑GCCGGCCCGCCCCGCCGCCCGCCACGTCGCCAATACCGCCATGCGGCACCAAGGCCAGAACcgcgg                                                                         >  1:138432/1‑134 (MQ=255)                                                              cTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCCACAAGGCCAGATCCtcgtc                                                                        >  4:80641/1‑136 (MQ=255)                                                              cTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCtcgtc                                                                        >  4:209380/1‑136 (MQ=255)                                                              cTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCAGGG‑CCAGGCCGGCACCGCCGCCGTTCAGTTCGCCAATACCGCCTTGCGGCAACAACGCCAGATCCtcgtg                                                                        >  4:362328/1‑135 (MQ=255)                                                              cTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGGAACCCTGGG‑CCCGGCCGGCACCGCCGCCGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCtcgg                                                                         >  2:286658/1‑134 (MQ=255)                                                              cTTCGGCACTGAACGGGTCGCCGAAAAAGCGCTCCGCAGTGAGGCCTGGCCGGACGTGGTAACCCTGTG‑CCAGGCCGGCACCGCCGCCGTAAAGTTAGGCAATAACGCCTTGGGGCAACAAGGCCAGATCCtcgtc                                                                        >  4:115380/1‑136 (MQ=255)                                                                   gCACTGAACGGGTCGGCGCCATAGAGCTCGGCAGTGAGGCATGGCCGGTCGTGGGACCCCTGGG‑CCAGGCCGGAACCGCCGACCTACAGTTCGCCCATACCGCCTATAGGCACCACTGCCAGATACACGGCGAgg                                                                    >  6:340303/1‑135 (MQ=255)                                                                     aCTCAACGGGTCGGCGAAAAAGCGCGCGGCAGGGCGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCCGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGAt                                                                  <  6:122033/135‑1 (MQ=255)                                                                          aCGGGTCGGCGACAAAGCGCTCGGCCGGGAGGCCTGGCCGGGCGTGGTAACCCGGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAgg                                                             <  7:265355/135‑1 (MQ=255)                                                                           agggTAGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAGCACAGTG‑CCAGGCCCGCAACGCCGACGTACCGTTACGAAATACCGGCGTGGCGCAACAAGGCCAGCTCCACGTCGAGGAGGTAgga                                                            >  2:112198/2‑134 (MQ=255)                                                                            ggggCGGCGACAACGCGCGGGCCAGTGAGGCGGGGCCGGGCG‑GGCAACCCCGGGCCCCGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGcc                                                           <  7:326924/135‑1 (MQ=255)                                                                            gggTCGGCGCCAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCGGGC‑CCCGGCCGGCACCGCCGACGTACAGTTCGCCAATACAGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGcc                                                           <  8:329512/135‑1 (MQ=255)                                                                               tCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCCGGCCGGCACCGCCGCCGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTAc                                                       >  4:184716/1‑136 (MQ=255)                                                                                 ggCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCCGGCCGGCACGGCCGCCGTTCACTTCGCCAATACCGCCGTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGGAGGCCGTACg                                                      >  2:149030/1‑135 (MQ=255)                                                                                 ggCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCCCCGCCGACGTACAGTTCGCCAATACCGCCGTGCGGCCACCAGGCCACATCCGCGTCGAGGATGTAGGCCGTACg                                                      >  5:109627/1‑135 (MQ=38)                                                                                 ggCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACCAGGCCAGATCCTCGTCGAGGAAGTAGGCCGTACg                                                      >  8:359232/1‑135 (MQ=255)                                                                                    gACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCACGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCCGCAACAACGCCAGATCCGCGTCGAGGATTTAGGCCGTACGGCt                                                   >  5:123591/1‑135 (MQ=39)                                                                                      cAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGGTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTAc                                                 >  8:279768/1‑135 (MQ=255)                                                                                          gcgcTCGGCAGGGAGGCCTGGCCGGGCGGGGAAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATg                                            <  3:81482/136‑1 (MQ=255)                                                                                           cgcTCGGCAGTGAGGCCTGGCCGGTCGTGGTTACCCGGGG‑CCCGGCCGGCACCCCCGCCGGTCAGTTGGGCAAAACCCCCTTGCGGCAACCAGGCCCGATCCACGTGGAGGGTGTTTGCCGGACCGCTACCGaag                                            >  6:249940/1‑133 (MQ=255)                                                                                           cgcTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCCCCGCCGCCGTACAGTTCGCCAATACCGCCGTGCGGCAACCAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATg                                            >  2:207951/1‑135 (MQ=255)                                                                                           cgcTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATg                                            >  2:282444/1‑135 (MQ=255)                                                                                                ggCAGTGAGGCCTGGCCGGTCGTGGTTACCCTGGG‑CCAGGCCGGCCCCGCCGACGGACAGTTCGCCAATACCGCCGTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGAcgcg                                       >  5:359077/1‑135 (MQ=255)                                                                                                   aGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCCCCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGCCGCGGcc                                    >  7:216544/1‑135 (MQ=255)                                                                                                       aGGCCGGGCCGGTGGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCg                               <  4:145407/136‑1 (MQ=255)                                                                                                        ggcggggCGGGGCGTGG‑AACCCGGGGCCCCGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCg                               <  1:151000/130‑1 (MQ=255)                                                                                                        ggCCTGGCCGGTCGTGGTAACCCTGGG‑CCCGGCCGGCCCCGCCGGCGTACAGTTCGCCAATACCCCCGTGCGGCCACCAGGCCAGATCCGCGGCGAGGATGTAGGCCGGACGGCTACCGATGACGCGGGCgcgcg                               >  6:99750/1‑131 (MQ=255)                                                                                                               ccGGTCGTGGTAACCCTGGG‑CCAGGCCGGCCCCGCCGACGTACAGTTAGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCGCGGCGAGGGTGTAGGCCGTACGGCTACCGCTGACGCGGCCGAGCGGTCCGCt                        >  7:119289/1‑135 (MQ=255)                                                                                                                gtggggTGGTACCCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGc                      <  4:242616/131‑1 (MQ=255)                                                                                                                              cgggg‑CCCGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTgg         <  1:232740/133‑1 (MQ=255)                                                                                                                              cTGGG‑CCAGGCCGGCACCGCCGACGTACAGATCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGc        <  3:60242/136‑1 (MQ=255)                                                                                                                              cTGGC‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTgg         <  1:139702/135‑1 (MQ=255)                                                                                                                               tGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGc        <  7:279768/135‑1 (MQ=255)                                                                                                                               tGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGc        <  8:341440/135‑1 (MQ=255)                                                                                                                                ggg‑CCAGGCCGGCCCCGCCGACGTCCAGTTCCCCAATACCGCCTTGCGGCAACAAGGCCAGAACCTCGGCGAGGATGTAGGCCGTACGGCAACCGATGCCGCGGCCGCTCGGTCCGCTGCCAAGGTCAGCTGGCaa      >  3:86951/1‑136 (MQ=255)                                                                                                                                    ccAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGCAAgg    >  5:22815/1‑135 (MQ=255)                                                                                                                                     cAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGCAAGGc   >  2:354874/1‑135 (MQ=255)                                                                                                                                     cAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGCAAGGc   >  7:200798/1‑135 (MQ=255)                                                                                                                                     cAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGCAAGGc   >  7:170952/1‑135 (MQ=255)                                                                                                                                     cAGGCCGGCACCGCCGACATACAGTTCGCCAATACCGCCt                                                                                                  >  7:233169/1‑40 (MQ=255)                                                                                                                                     cAGGCCGGCACCGCCGACATACAGTTCGCCAATACCGCCt                                                                                                  <  8:233169/40‑1 (MQ=255)                                                                                                                                      aGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCTGCCGATCGGTACGCTTACAAGGTCTGCTGGCAAGGcc  >  5:148258/1‑135 (MQ=255)                                                                                                                                      |                                                                                                                                       TACTCCACCAGGCCATCGGTACCCAGGCGTACCAGGTCGCCGGTACGGTACAGGCGCCCGCCTTCGGCACTGAACGGGTCGGCGACAAAGCGCTCGGCAGTGAGGCCTGGCCGGTCGTGGTAACCCTGGG‑CCAGGCCGGCACCGCCGACGTACAGTTCGCCAATACCGCCTTGCGGCAACAAGGCCAGATCCTCGTCGAGGATGTAGGCCGTACGGCTACCGATGACGCGGCCGATCGGTACGCTGCCAAGGTCTGCTGGCAAGGCC  >  KT2440/4817289‑4817555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑