| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,689,816 | A→C | 69.8% | S236R (AGC→CGC) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,689,816 | 0 | A | C | 69.8% | 5.5 / 4.6 | 26 | S236R (AGC→CGC) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (3/15); total (9/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
GGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAG > KT2440/5689693‑5689947 | ggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCGCGTGCCCCACATCACCAcc‑cccccggtc > 1:243710/1‑130 (MQ=255) tGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGCTCACCAGC‑GCCCGTGGCGCGCGGc > 8:258586/1‑135 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGGGCACTTAAGCCACATAACCACC‑GCCCCTTGCGCCCGGCGGATCg > 4:329494/1‑136 (MQ=255) ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGGGGCAGTCAGCCACAAAACACGC‑CCCCGTGTCGCGCCGCGGAAAgccca > 6:283454/1‑135 (MQ=255) gggggCCGGCGGGGAACCCCGCAGCGCCCCCAAACGGGGGCCGGGGCGCGCAACCGGGTGGGGGGGGACCCGCGGGGGGGGCGGGCGCAGTACCGCCAGAACCCCCCC‑GCCGGTGGCGCGCGGCTGATCgcccag < 6:362330/132‑1 (MQ=255) ccTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGC‑GCCCGTGGCGCGCGGGGGGTCgcccaggcccc > 7:74020/1‑134 (MQ=255) ggCAGCCCGGAGCGCGCCGAAAGCGGGGCCCGGGCGCGCAACCGGCGGCGGGTGCAACAGCGCGGGGGGCGGGCGCACTTCCCCCAGAACACCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg < 7:169989/135‑1 (MQ=255) ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCACGTCAGCCAGATCCCCACC‑GCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCCGCCGTGGGCCTg > 2:68987/1‑135 (MQ=255) ccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGGGGGGGCCCTTCAGCCCGATCACCCCC‑GCCCCGGGCGCGCGGGGGCTCGCCCCCGCCCCCGCCCTggggcgg > 1:111976/1‑131 (MQ=255) ggAGCGCGCCGAAACCGGGGCCCTGGCTCGCACCCTGCTGCGGGGGGAACAGCGCGGCGTGCGTGCGAACTTCAGCCAGATCACCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc < 6:80774/135‑1 (MQ=255) gcgcCGAACCGGGGGCCCTGGCGCGCAACCTGCTGCTGGGGCACCAGAGCGGGGGGCGTGCGCACTTCAGCCAGATCACCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 3:30707/136‑1 (MQ=255) gcgcCGAAACCGGGGCCCTGGCGCGCAACCTGCGGGGGGTGCAACAGTGCGGGGGGCGGGCGCACTGCACCCATATCACCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 3:26036/136‑1 (MQ=255) cgcCGAAACGGGGGCCCTGGCGCGAAACCTGGGGCGGGGGCAACAGCGCGGCGGGCGTGCGCACTGCCCCCCCACCCCCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 1:228882/135‑1 (MQ=255) cgcCGAAACCGTGGCCGTGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGGGGGCGGGCGCACTTCAGCCAGATCACCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 5:80661/135‑1 (MQ=255) gaaccggTGGCCCGGGCGCGCAACCTGGTGCGGGGGCAACAGCGCGGCGTGCGTGCGCACTGCCCCCACAGCCCCCCC‑GCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 4:15362/130‑1 (MQ=255) gTGGGCCGGGCGCGCAACGGGGGGGGGGGGGAACCGGGGGGGGGGGGTGGGGACTTCAGCCACCTCCCCCGCGGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:281025/136‑1 (MQ=255) gTGGCCCTGGCGCGAACCCGGCGGCTGGTGGAACTGCGCGGGGTGCGTGCGCACTTCCGCCAGATCCCCCCC‑GCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:22193/135‑1 (MQ=255) cggcgTGCGTGCGCACTTCACCCAGATCACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 8:74020/135‑1 (MQ=255) gtgggggcgcACTCCAGCCACAGCCCCCGG‑GCCCGGGGCCCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 4:13111/130‑1 (MQ=255) ggcgggcgCAGTACCGCCAGATCCCCCGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 8:4167/134‑1 (MQ=255) gggggcgcACTGCAGCCCGACCACCCGC‑GCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt < 6:310371/131‑1 (MQ=255) gcgcACTTCACCCATAACACCAGC‑GCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 5:139096/135‑1 (MQ=255) cACCCGC‑GCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCGGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg < 4:112558/136‑1 (MQ=255) cACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCa > 7:45400/1‑135 (MQ=255) cACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCa > 2:259309/1‑135 (MQ=255) aCCCGC‑CCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCCGCTGATCCTCACGGACGAGTAGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg < 6:242053/135‑1 (MQ=255) | GGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGC‑GCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAG > KT2440/5689693‑5689947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |