Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,780,7340TG52.2% 9.2 / 28.6 23intergenic (+124/‑1)ahpC_2/ahpFAlkyl hydroperoxide reductase C/Alkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base T (5/6);  new base G (11/1);  total (16/7)
Fisher's exact test for biased strand distribution p-value = 2.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACGACCTGCTG  >  KT2440/2780608‑2780854
                                                                                                                                |                                                                                                                        
tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGGGGCCACGGCGGGGCGGGTTTGTATGCGCGTTTTGTAAAAGGCATCGCCCGGCTGTTggg                                                                                                                  >  7:202003/1‑134 (MQ=255)
          cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGGTTTTGTTTCCGAGTTTTGAAAAAGGAATCGCCCGGATGTTGGCCGCCACGCt                                                                                                         >  6:190102/1‑134 (MQ=255)
          cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCGGGGCGTGTTTGTTTCCGCGGTTTGGAAAAGGAATCGCCCGGTTGTTGGGCGCCCCGcg                                                                                                         >  7:104903/1‑133 (MQ=255)
          cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCGGGGCGGTTTTGTTTCCGAGTTTTGAAAAATGCAAAGCCCGGTTGTTTGGCGCCACgag                                                                                                         >  6:252768/1‑132 (MQ=255)
          cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCCGGCGCCCGCGCCTGGGCGTTTTTGTTTACCAGGTTTTTAAAAAGAATCGCCCGGATTTTGGACGCCACGcg                                                                                                         >  8:14803/1‑133 (MQ=255)
          cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGCGACCTCGCCTGGGCGGTGTTGTAGCCGAGGTTTGAAAAAGGATTCGGCCGGATTGTGGACGCCCCGcct                                                                                                        >  3:338759/1‑133 (MQ=255)
          cccATAACGCGGGCGGTAAGCAGCAGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCCACCTAGCCTGGGGGTTTTTGTATACGAGTTTTTAAAAATGCATGGCCCCTAAGTTGGGCGCCAccgg                                                                                                         >  5:242577/1‑131 (MQ=255)
           ccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAAACCCCGGGGGCCCACGCCAGGGCGGGTTTTGTTACGGGGTTTTGAAAAGGAATCGCCCCGATGTTGGTCGcccccggg                                                                                                        >  2:253579/1‑128 (MQ=255)
                 cgcgAGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGGTTTGAAAAAGGGATCGCCCGTATGTTGGACGCCACGCTTAAATCg                                                                                                  >  4:172708/1‑134 (MQ=255)
                       cggTACGCCGCCGCCAAAGCTGAAGTACCAACCGCCCCGTAAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGa                                                                                           <  1:112699/135‑1 (MQ=255)
                       cggTAAGCAGCCGCCAATCGTGAAGGACCTACCGCCCGGTCAAACGCCCGGGCGACCGGGGCTGGGCTTTTTTGTATCCTAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACt                                                                                             <  6:237761/133‑1 (MQ=255)
                        ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCCTGGGCGTTTTTGTTTGCGAGTTTTGAAAAAGGAATCGGCCGGTTGGTGGCCGCCCCGCGTAAATCGCAACTGa                                                                                           >  3:354147/1‑134 (MQ=255)
                        ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCCGGGGCGGTTTTGTTTGCGCCGTTTGAAAAATGAATAGCCCGGATGTTGGTCGCCCCGCCTAAAACGCAAcagt                                                                                           >  7:289758/1‑131 (MQ=255)
                                    ccAAAGCTGATGTACCTCCCGCCCCGGCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGa                                                                              <  4:240329/135‑1 (MQ=255)
                                                       cGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGATTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGAt                                                            <  7:188279/134‑1 (MQ=255)
                                                          cccGTCAAACGCCCGGGCGCCCTCGCCGGGGGCGTTTTTTTTAAGAGTTTTGAAAAAGGAATAGCCCGGATGTTGGCCGCCACGCGTAAAAAGGAACAGAAAACCAACATGGAGCGGGTCACCCACCCgcacaa                                                         >  8:76930/1‑129 (MQ=255)
                                                             gTCAAACCCCCGGGCGACCTCGCCTGGGCGTTTTTGTTTCCGCGTTTTGTAAAAGGAATAGCCCCGATGTTGGGCGCCCCGCGGTAATAGCCACTGAAAACCAACATTGTGCGGGGGACCCCCCCGATCGCGAta                                                     >  6:64949/1‑134 (MQ=255)
                                                                           cgcccTCGCCGGGGCGTTTTTGTTGCCGAGGTTGGCAAAAGGAATCGCCCGGATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTcga                                       <  5:5013/132‑1 (MQ=255)
                                                                                 cGCCTGGGCGTTTTTGTTTCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCCCGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGCTCGAGATCGTTGCGTCCCTCGACGACgg                                 >  8:194556/1‑135 (MQ=255)
                                                                                                           ttttCAAAAAGTACTCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACg          <  1:253579/134‑1 (MQ=255)
                                                                                                                  aaaGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACGACctgctg  >  7:45134/1‑135 (MQ=255)
                                                                                                                   aaGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGt                         <  7:269161/111‑1 (MQ=255)
                                                                                                                   aaGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGt                         >  8:269161/1‑111 (MQ=255)
                                                                                                                                |                                                                                                                        
TAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACGACCTGCTG  >  KT2440/2780608‑2780854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: