Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 2,780,734 | 0 | T | G | 52.2% | 9.2 / 28.6 | 23 | intergenic (+124/‑1) | ahpC_2/ahpF | Alkyl hydroperoxide reductase C/Alkyl hydroperoxide reductase subunit F |
Reads supporting (aligned to +/- strand): ref base T (5/6); new base G (11/1); total (16/7) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.72e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-03 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACGACCTGCTG > KT2440/2780608‑2780854 | tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGGGGCCACGGC‑GGGGC‑GGGTTTGTATGCGCGTTTTGTAAAAGGCATCGCCCGGCTGTTggg > 7:202003/1‑134 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GGTTTTGTTTCCGAGTTTTGAAAAAGGAATCGCCCGGATGTTGGCCGCCACGCt > 6:190102/1‑134 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑GGGGC‑GTGTTTGTTTCCGCGGTTTGGAAAAGGAATCGCCCGGTTGTTGGGCGCCCCGcg > 7:104903/1‑133 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑GGGGC‑GGTTTTGTTTCCGAGTTTTGAAAAATGCAAAGCCCGGTTGTTTGGCGCCACgag > 6:252768/1‑132 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCCGGCGCCCGCGCC‑TGGGC‑GTTTTTGTTTACCAGGTTTTTAAAAAGAATCGCCCGGATTTTGGACGCCACGcg > 8:14803/1‑133 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGCGACCTCGCC‑TGGGC‑GGTGTTGTAGCCGAGGTTTGAAAAAGGATTCGGCCGGATTGTGGACGCCCCGcct > 3:338759/1‑133 (MQ=255) cccATAACGCGGGCGGTAAGCAGCAGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCCACCTAGCC‑TGGGG‑GTTTTTGTATACGAGTTTTTAAAAATGCATGGCCCCTAAGTTGGGCGCCAccgg > 5:242577/1‑131 (MQ=255) ccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAAACCCCGGGGGCCCACGCC‑AGGGCGGGTTTTGTTACGGGGTTTTG‑AAAAGGAATCGCCCCGATGTTGGTCGcccccggg > 2:253579/1‑128 (MQ=255) cgcgAGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGGTTTGAAAAAGGGATCGCCCGTATGTTGGACGCCACGCTTAAATCg > 4:172708/1‑134 (MQ=255) cggTACGCCGCCGCCAAAGCTGAAGTACCAACCGCCCCGTAAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGa < 1:112699/135‑1 (MQ=255) cggTAAGCAGCCGCCAATCGTGAAGGACCTACCGCCCGGTCAAACGCCCGGGCGACCGGGGC‑TGGGC‑TTTTTTGTATCCTAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACt < 6:237761/133‑1 (MQ=255) ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCC‑TGGGC‑GTTTTTGTTTGCGAGTTTTGAAAAAGGAATCGGCCGGTTGGTGGCCGCCCCGCGTAAATCGCAACTGa > 3:354147/1‑134 (MQ=255) ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCC‑GGGGC‑GGTTTTGTTTGCGCCGTTTGAAAAATGAATAGCCCGGATGTTGGTCGCCCCGCCTAAAACGCAAcagt > 7:289758/1‑131 (MQ=255) ccAAAGCTGATGTACCTCCCGCCCCGGCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGa < 4:240329/135‑1 (MQ=255) cGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGATTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGAt < 7:188279/134‑1 (MQ=255) cccGTCAAACGCCCGGGCGCCCTCGCCGGGGGC‑GTTTTT‑TTTAAGAGTTTTGAAAAAGGAATAGCCCGGATGTTGGCCGCCACGCGTAAAAAGGAACAGAAAACCAACATGGAGCGGGTCACCCACCCgcacaa > 8:76930/1‑129 (MQ=255) gTCAAACCCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTTTCCGCGTTTTGTAAAAGGAATAGCCCCGATGTTGGGCGCCCCGCGGTAATAGCCACTGAAAACCAACATTGTGCGGGGGACCCCCCCGATCGCGAta > 6:64949/1‑134 (MQ=255) cgcccTCGCC‑GGGGC‑GTTTTTGTTGCCGAGGTTGGCAAAAGGAATCGCCCGGATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTcga < 5:5013/132‑1 (MQ=255) cGCC‑TGGGC‑GTTTTTGTTTCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCCCGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGCTCGAGATCGTTGCGTCCCTCGACGACgg > 8:194556/1‑135 (MQ=255) ttttCAAAAAGTACTCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACg < 1:253579/134‑1 (MQ=255) aaaGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACGACctgctg > 7:45134/1‑135 (MQ=255) aaGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGt < 7:269161/111‑1 (MQ=255) aaGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGt > 8:269161/1‑111 (MQ=255) | TAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCGCGAAGTCCCGCGAATTGCACGACCTGCTG > KT2440/2780608‑2780854 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |