Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,264,1340TG41.9% 13.0 / 12.4 32E336D (GAA→GAC) CJ019_03065Metal reductase
Reads supporting (aligned to +/- strand):  ref base T (2/16);  new base G (11/2);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 1.01e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTACTGCAACTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATA  >  KT2440/3264010‑3264266
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cGTACTGCAACTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑CTtgg                                                                                                                            >  8:237008/1‑135 (MQ=255)
 gTACTGCAACTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTGGGT‑Gtttgg                                                                                                                           >  2:149077/1‑132 (MQ=255)
         aCTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCg                                                                                                                   <  4:290955/135‑1 (MQ=255)
         aCTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑CTTGGCTGGCATCg                                                                                                                   >  4:278767/1‑135 (MQ=255)
         aCTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGAATGCCTGGTTGCAGGCGATGCAGGCGCGCCCGGCTTCGACCTGGCCGCGTGGGTG‑CTTGGCTGGCATCg                                                                                                                   >  8:330952/1‑135 (MQ=255)
              cGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGa                                                                                                              <  2:105142/135‑1 (MQ=255)
               gCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑CTTGGCGGGCATCGACGGAt                                                                                                             <  3:127006/135‑1 (MQ=255)
                cccGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑CTTGGCTGGCATCGACGGATc                                                                                                            >  4:136203/1‑135 (MQ=255)
                   ggTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑CTTGGCTGGCATCGACGGATCGCa                                                                                                         <  3:278767/135‑1 (MQ=255)
                    gTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTGGGT‑GTTTGGTGTCATCGGCGGGTCgagt                                                                                                        >  3:39437/1‑132 (MQ=255)
                     tttCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGa                                                                                                       <  7:310580/135‑1 (MQ=255)
                         cGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAg                                                                                                   <  7:25804/135‑1 (MQ=255)
                                  gATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTGGGG‑GTTGGGTGGCCTAGAGGGGTCTCAGCTGACCGCCCgggg                                                                                          >  5:350129/1‑134 (MQ=255)
                                  gATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTGGGG‑GTTGGCGGGCCTCGCCGGCTCGCAGATCAGCGCCCGGGt                                                                                          >  1:218131/1‑135 (MQ=255)
                                  gATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑CTTTGGTGGCATCGGCCGATCGCCCGACAGCGCCCcggg                                                                                          >  1:66860/1‑134 (MQ=255)
                                   aTGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTc                                                                                         <  8:120260/135‑1 (MQ=255)
                                       aGGAAAGGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCTTTCGGT‑CTTGGCTGGCATCGACGGAGCGCAGATCAGCGCCCGGGTCATg                                                                                      <  8:319136/134‑1 (MQ=255)
                                             tGGCCAAGCCACGATGGAAATGGCCGATGCAGGCCTGGTGGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGAGCAGCGCCCGGGTCATGCCGcaca                                                                               <  1:149077/135‑1 (MQ=255)
                                                   aGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCTATGCAGGCGCGCACGTCTTCGACCTGGCCGGGTTCGGT‑CTTGGCGGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAg                                                                         <  5:326235/135‑1 (MQ=255)
                                                       aCGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGGTCGGT‑GGTGGCTGGGCTCGACGGATCGCCGATCAGCCCCCGGGGCATGGCGCACACCTCAGCCTg                                                                     >  5:106059/1‑135 (MQ=255)
                                                        cGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTgg                                                                    <  7:20124/135‑1 (MQ=255)
                                                        cGATGGAAATGGCCGATGCAGGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCAGCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTgg                                                                    <  8:264929/135‑1 (MQ=255)
                                                              aaaTGCCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTGCGGT‑CTTGGCTGGCATCGACGGATCGCAGAGCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCcgcg                                                               <  5:110883/134‑1 (MQ=255)
                                                                        tgcatgcCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCGGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCa                                                    <  8:111543/135‑1 (MQ=255)
                                                                           atcccTGTTCGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCt                                                 <  7:205228/132‑1 (MQ=255)
                                                                                     gCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTGGGT‑CTTGGCTGGCATCGACGGGTCGCCGATCAGCGCCCGGGTCATGCCGCCCACATCAGCCTGGCCGCGGGCCAGGCTCAGCTCGGCTTCCTg                                       >  7:43038/1‑135 (MQ=255)
                                                                                                          gTCTTCGACCTGGCCGCGGTGGGG‑GTTGGCTGGGCGCGACGGCTCGCAGATCAGCGGCCGGGGGATGGCGCCCACATCAGCCTGGCCGCGGGGCAGGCTCACCTCGGCGTGCGGGGGGGGGTTGATGCGGCCggg                  >  6:248400/1‑134 (MQ=255)
                                                                                                             tgcGACCTGGCCGCGTGGGGG‑CTTGGCGGCCATCGACGGTTCGCCCATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGAc               <  5:122997/133‑1 (MQ=255)
                                                                                                                  ccTGGCCGCG‑GCGGTCCTTGGCGGGAATCGCCGGAGCGCAGATCCGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACa          <  1:311026/135‑1 (MQ=255)
                                                                                                                    tGGCCGGGTTCGGT‑CGTGGCGGGCATCGACGCATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAgg        <  2:161522/135‑1 (MQ=255)
                                                                                                                       ccGCGGTGGGT‑GGTGGGTGGGCTCGCCGGTTAGCCGCTCAGCGCCCGGGGCATGCGGGCCCCATCAGCCTGGCCGCGGGCCAGGCTCAGCTCGGCGTCCGGGGGCGGGGTGCTGGGCCCGGGGTCGCACAcgggg     >  2:254833/1‑135 (MQ=255)
                                                                                                                          cgTGCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATa  <  7:93399/135‑1 (MQ=255)
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CGTACTGCAACTCACGCCCGGTTTCCGGGCGCTGGATGCAGGAAATGGGCAAGCCACGATGGAAATGGCCGATGCATGCCTGGTTGCAGGCGATGCAGGCGCGCACGTCTTCGACCTGGCCGCGTTCGGT‑CTTGGCTGGCATCGACGGATCGCAGATCAGCGCCCGGGTCATGCCGCACACATCAGCCTGGCCGCGGGCCAGGATCAGCTCGGCTTCCTGGGGCTGGTTGATGCGCCCGGTGACGAACAGGGGGATA  >  KT2440/3264010‑3264266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑