| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,515,112 | 0 | T | G | 59.1% | ‑3.0 / 14.3 | 22 | intergenic (+36/+365) | CJ019_03333/vldH | hypothetical protein/Validoxylamine A 7'‑phosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (8/1); new base G (13/0); total (21/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAAGCGCAGCGCCTTGCAACG > KT2440/3514986‑3515223 | cTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCgtttcagttt < 4:208155/134‑1 (MQ=255)cTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCgtgtcagttt > 3:303953/1‑134 (MQ=255) cAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGACGGTTGATACCCTGACCCGGCGTTTCAGTTTCTACGTCGGTgg > 8:345974/1‑134 (MQ=255) aaGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGc > 3:341942/1‑134 (MQ=255) cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACTCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCAtctc < 2:145230/135‑1 (MQ=255) cTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCAtctc > 4:45925/1‑135 (MQ=255) gcCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCCTCTcgcgcg > 8:294180/1‑135 (MQ=255) cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTTGCCATCTCGCGCGGCTGTgc > 1:32032/1‑135 (MQ=255) cGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTAAACCTCGGGGGCCCTCTCGCGCGGCGGTgc > 5:351309/1‑135 (MQ=21) aaCGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGGCAAATCCCGCCGCAGTGCGCCCGACGcc > 7:300234/1‑135 (MQ=18) aaCGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCgccgcc > 5:187372/1‑135 (MQ=255) acGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCCTCTCGCGCGGCTGTGCGCGCGCCCCCTGTTCGGTGa > 7:103960/1‑135 (MQ=255) acGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGa > 1:32835/1‑135 (MQ=255) aCAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGtt > 5:173240/1‑135 (MQ=255) cTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTGAACGTCGGTGGCCATCTCGCGCGGCAGTGCGCCCGACGCCAGTTCGGTGACCGCCAGTTGCGTTTCaac > 7:111679/1‑134 (MQ=37) cTACGACGCTTCGACCAGCTGAGCGAAGAAGTGACGGTGGATAACCTGACCCGGCGTTTCACTTTAAACGTAGGTGTCCATCTCGCCCGGAGTTTCCCGCGCCGCCAGTTCGGTTCCCGGCCCTTGCGTTTGAAg > 8:274111/1‑135 (MQ=16) tACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGGAGGTGGCCATCTCGCGCGGCTGTGCGCGCGACCCCTGTTCGGGGACCGCCCGTTGCGTTTCAAGc > 8:289912/1‑135 (MQ=37) acgacgCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTGAGTTTCAACGTCGGTGGCCCTCTCGCGCGGCGGTGCGCGCGCCGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCg > 6:324052/1‑135 (MQ=255) gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTGAACCTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACg > 6:143786/1‑133 (MQ=255) gCTTCGACCAGCTGAGCGAAGAAGTGCCGGTCGATATCCTGACCCGGCGTGTCAGATTAAACGTCGGTGGCCCTATCGACCGGCATTGCGCGCGCAGCCGGTTCGGTTAACCCCACTTGGGTTTCAAGCgccgcc > 8:204525/1‑135 (MQ=255) ttCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTGAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGccgg > 3:120536/1‑133 (MQ=255) gAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAagcgc > 3:357193/1‑135 (MQ=255) aGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGTCGGTGGCCCTCTCGCGCGGCTGTGCGCGCGCCGCCTGTTCGGTGACCGCCAGGTGCGTTTCAAGCGACGCCGGGTGGAAagcgca > 8:356300/1‑135 (MQ=38) cGGTGGATAACCTGACCCGGCGTGTCAGTTTCAACGGCGGTGGCCATCTCGCGCGGCTGTGCGCGCGCCGCCGGGTCGGTGCCCCCCAGGTGCGGTTCAAGCGACGCCTGGTGGAAAGCCCAGCGCCTTGCAAcc > 8:350760/1‑134 (MQ=37) | CTACGAGTACGACAAGGCCTTGCGCCTGGCCGCACTGGTCAACGAGAACAACGCCACGTACAGCCTTGCCTACGACGCTTCGACCAGCTGAGCGAAGAAGTGCCGGTGGATAACCTGACCCGGCGTTTCAGTTTCAACGTCGGTGGCCATCTCGCGCGGCTGTGCGCGCGACGCCTGTTCGGTGACCGCCAGTTGCGTTTCAAGCGACGCCTGGTGGAAAGCGCAGCGCCTTGCAACG > KT2440/3514986‑3515223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |