Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,880,2080AC45.2% 18.9 / 11.2 42G100G (GGT→GGG) CJ019_04574hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (9/14);  new base C (17/2);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 1.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGT  >  KT2440/4880074‑4880339
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gCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGGCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGcacccca                                                                                                                                      <  8:8476/135‑1 (MQ=255)
    ccccGGCATCTGCCCGCAGCCCCCCCTCCTCGATCGCACTGATCGCTCACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCg                                                                                                                                  >  7:239069/1‑135 (MQ=255)
    ccccGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCCCACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCCCGGCACCCCGCACCTTGCCGTCCTGATCACGAATCAGccccccccccc                                                                                                                                  >  2:278999/1‑134 (MQ=255)
            tCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGc                                                                                                                          >  1:202032/1‑135 (MQ=255)
                   gCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGCTCACGAATCAGCACCCCCCCCGGCGCCGGCccccccc                                                                                                                   >  3:207129/1‑134 (MQ=255)
                    cAGCCGCAGATCCTCGATCGACGTGATGGCTCACTGCTGGTCGATATCCGAGGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCTTCCTGATGACGAATCAGGACCCCACGCTGAGCCGGCACCACCgg                                                                                                                  <  2:171704/135‑1 (MQ=255)
                    cAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                  <  1:195007/135‑1 (MQ=255)
                    cAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                  <  7:264882/135‑1 (MQ=255)
                        cccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATGACGAATCAGCCCCCCCCCCGGCGCCGGCACCCCCCGcccc                                                                                                              >  1:170852/1‑133 (MQ=255)
                         ccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCt                                                                                                             <  3:332928/135‑1 (MQ=255)
                         ccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCt                                                                                                             <  2:6999/135‑1 (MQ=255)
                                        cACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCCCCGGCCGCGCA‑CCCCTCCCGGTCa                                                                                             >  4:361118/1‑135 (MQ=255)
                                           tGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCCGCCGCGCA‑CCCCTCCCGGTCCAcg                                                                                          >  8:226660/1‑135 (MQ=255)
                                                gCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCCCCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCCCCACCGGCCGCGCC‑CCCAACCCGTTCAACGCGGc                                                                                      >  6:100823/1‑134 (MQ=255)
                                                gCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCCGCGCCCGCACCCCCCGCCGCGCC‑CCCCTCCCCGTCCACGCGGc                                                                                      >  3:285818/1‑134 (MQ=255)
                                                    acTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGCGGC‑CCCAACCCCGTCAACCCGGCaaaa                                                                                  >  6:62024/1‑134 (MQ=255)
                                                      tGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCCGCCCCGGCACCACCGGCCGCGCC‑CCCCTCCCGTTCCACGCCGCcaaaaaa                                                                               >  2:291484/1‑135 (MQ=255)
                                                        cTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCCCCGGCCCCCCCCGCCGCGCA‑CCCATCCCCGTCAACGCGGCAAAAAAAgc                                                                             >  3:176809/1‑135 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCCGCACCTTGCCGTCCTGATCACGAATCAGCCCCCCCCCCGGCGCCCGCCCCACCGGCCGCGCACCCCAACCCG‑TCAACGCCGCAAAAAAAaccg                                                                           >  2:353028/1‑135 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCCCCGGCCGCGCC‑CCCCTCCCGGTCCACGCGGCAAAAAAAgccc                                                                           >  4:87839/1‑134 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCCCCCGCCGCTCC‑CCCCACCCCTTCAACCCGGCAAAAAAAgccg                                                                           >  6:252407/1‑135 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGCTCC‑CCCCTCCCGTTCAACGCGGCAAAAAAAgccg                                                                           >  8:210601/1‑135 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGCGCC‑CCCATCCCGTTCCACGCGGCAAAGAAAgccc                                                                           >  3:273335/1‑134 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCCCCCCCGGCCGCGCC‑CCCCTCCCGTTCCACGCGGCAAAAAAAgccg                                                                           >  1:38299/1‑135 (MQ=255)
                                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCAACCCGCGCCCGCACCACCCGCCGCGCC‑CCCATCCCCGTCAAACCGGCAAAAAAAgccc                                                                           >  8:92518/1‑134 (MQ=255)
                                                             cgATATCCGACGTATCGCCGCTGACCCCCCCGGCACCCAGCACCTTGCCGTCCTCAACCCGAATCAGCCCCCCCCCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAgccggcc                                                                        <  6:303014/135‑1 (MQ=255)
                                                                   ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCCCCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCCCCACCGGCCGCGCC‑CCCCTCCCGTTCACCGCGGCAAAAAAAGCCGGCCgctgcg                                                                  >  2:226603/1‑134 (MQ=255)
                                                                   ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGGCGCTCA‑CCCCTCCCGGTCCACGCGGCAAAAAAAGCCGGCCgctgcg                                                                  >  6:106432/1‑134 (MQ=255)
                                                                   ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGCTCC‑CCCATCCCGGTCCACGCGGCAAAAAAAGCCGGCCcctgcg                                                                  >  2:6918/1‑134 (MQ=255)
                                                                   ccGACGTATCGCCGCTGATCCCCACGCCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGCGCA‑CCCATCCCGTTCAACGCGGCAAAAAACGCCGGCCgctgct                                                                  <  5:96443/135‑1 (MQ=255)
                                                                   ccGACGTAGCGCCGATGATCCCCACGGCACCCAGCACCTTGCCTTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCgctgct                                                                  <  3:117422/135‑1 (MQ=255)
                                                                     gACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCCTCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCtgctgc                                                                >  2:219250/1‑135 (MQ=255)
                                                                          aTCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCCCCGGCCGCGCC‑CCCCGCCCCGTCAACGCGGCCAAAAAAGCCGGCCGCTGCGgcgccgc                                                           >  3:25058/1‑132 (MQ=255)
                                                                                    aTCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGCGCA‑CCCCTCCCGGTCAACGCGGCAAAAAAAGCCGGCCGCGGCTGCGCCTCCAGCGCCCGc                                                 >  3:103048/1‑135 (MQ=255)
                                                                                        ccACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAgc                                             <  4:314323/135‑1 (MQ=255)
                                                                                        ccACGCCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGCCCGCTCA‑CCCATCCCGTTCAACGCCGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAgc                                             <  7:92518/135‑1 (MQ=255)
                                                                                                  cAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                   <  3:107492/135‑1 (MQ=255)
                                                                                                  cAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAACCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                   <  1:65024/135‑1 (MQ=255)
                                                                                                  cAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCAACCCGTTCAACGCGGCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                   <  4:207129/135‑1 (MQ=255)
                                                                                                   aGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCCCCACCGGCCGCTCA‑CCCCTCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCGGCGCCTCCCGCGCCCGCACGCGCGCGCCCTTg                                  >  8:188/1‑135 (MQ=255)
                                                                                                             cGTCCTCAGCACGAATCCGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCACCGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCaa                        <  2:202032/135‑1 (MQ=255)
                                                                                                                  tGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCAACCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCa                   <  1:78477/135‑1 (MQ=255)
                                                                                                                  tGATCACGAATCAGCACACCCCCCGGCGCCGGCACCACCGGCCGCACA‑CCCAACCCCTTACACGCGGCAAAAAAAGCCGGCCGCAGCTGCGCATCCAGCGCCAGCAAGCCCGACCCCATGCCCACGGCAATCGCa                   >  7:279374/1‑135 (MQ=255)
                                                                                                                        cGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCa              <  8:239069/134‑1 (MQ=255)
                                                                                                                              ccacccccccccGGCGCCGCCCCCCCCGCCCGCCCA‑CCAATCCCGTGCACCGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCTCAGGGCAATCGCACCCCAGGCTTTg       <  5:330847/131‑1 (MQ=255)
                                                                                                                                   cccacccGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGt  <  7:304372/135‑1 (MQ=255)
                                                                                                                                   cccacccGGCCCCGCCACCACCGGCCGCTCA‑CCCATCCCGACCACCGCGCCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGt  <  1:291484/135‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCTCA‑CCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGT  >  KT2440/4880074‑4880339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑