Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,689,8160AC56.2% 7.7 / 24.5 36S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (8/6);  new base C (2/16);  total (10/26)
Fisher's exact test for biased strand distribution p-value = 8.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAA‑CCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  KT2440/5689683‑5689948
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ggCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAA‑CCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGATCACCac                                                                                                                                     >  8:307840/1‑134 (MQ=255)
                  ggCCAGCTTCCCCGCCCTCCCGGGCAACCCGGAGCGCGCCGAAACCGTGGCCCGGGCGCGCAA‑CCTCCTGCTGGGGCAACAGAGCGGCGGGCGGGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCt                                                                                                                   <  4:110996/135‑1 (MQ=255)
                      aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCCTGGCCCTGGCGCGCAA‑CCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCGCTTAAGCCCCATCACCAGCGCCCCTGGCCCGCGGCTGATc                                                                                                               >  7:52560/1‑135 (MQ=255)
                            cgcGGCCTGCCGGGCAGCCCGGAGAGCGCCGAACCCGGGGCCCGGGCGCGCAA‑CCGGCGGCTGGTGGACCCGCGCGGCGTGCGGGCGCACTGCCGCCAGACCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                         <  4:251355/133‑1 (MQ=255)
                             tCGGCCGGCCGGGCACCCCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAA‑CCTGCTGCTGGGGCAACAGCGCGGCGGGCGGGCGCACTTCCCCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCgcccagg                                                                                                        <  4:175173/135‑1 (MQ=255)
                              cggccggcGGGGCACCCCGCCGCCCGCCCAACCCGTGGCCCGGGCGCGAAA‑CGGGCTGCGGGGGGACCCGCGGGGCGGGCGTGCGCACTTCACCCCGAGCCCCGGCGCCGGGGGCGCGCGGCTGATCgcccaggc                                                                                                       <  2:46292/135‑1 (MQ=255)
                                gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAA‑CCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCCCATCCGCCACAACCACCCCGCCCCTGGCGCGCGGCGGTTAgccccggccc                                                                                                     >  1:48369/1‑135 (MQ=255)
                                                 gagaGCGCCCAAACCGGGGCCGGGGCG‑GCAACCCGGGTGGTGGGGCAACAGCGGGGGGGGGGGGCGCACTCCCCCCAGAACCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg                                                                                    <  8:313463/135‑1 (MQ=255)
                                                      cgcCGAAACGGTGGCCCGGGCGCGCAC‑CCTGCGGCTGGGGGAACTGAGGGGCGGGCTTGCGCACTTCACCCAGACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                               <  4:115794/135‑1 (MQ=255)
                                                           aacccGTGGCCCGGGCGCGCAC‑CCTGCGGCTGGTGCAACCGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          <  7:270019/132‑1 (MQ=255)
                                                                ggggCCCTGGCGGGAAA‑CCTGCTGGTGGGGCAACAGTGCGGGGTGCGTGCGCAGTTCAGCCATATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  4:147146/133‑1 (MQ=255)
                                                                ggggCCCTGGCGCGAAA‑CGTGGGGGTGGTGGACCCGGCGGGGGGGCGGGCGCACTTCGCCCATAACACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  6:262372/133‑1 (MQ=255)
                                                                gTGGCCCTGGGGCGCAA‑GCTGCTGCTGGTGCAACTGAGGGGGGTGGGTGGGCAGTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  7:215382/135‑1 (MQ=255)
                                                                 gggCCCTGGCGCGCAA‑CCTGCTGCGGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAg                                                                    <  7:266730/134‑1 (MQ=255)
                                                                 gggCCCGGGCGCGCAA‑CCTGCTGCTGGTGGAACAGCGCGGCGGGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAg                                                                    <  7:307840/134‑1 (MQ=255)
                                                                     ccgggCGCGCAA‑CCGGCTGCGGGGGGAACAGAGCGGCGGGCGTGCGCACTGCAGCATGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                <  8:101898/132‑1 (MQ=255)
                                                                         gcgcgcAA‑CCGGCGGCTGGGGGACCAGTGCGGCGGGGGGGCGAACTTCCGCATCCTCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                                            <  7:87174/135‑1 (MQ=255)
                                                                           gcgcAA‑CCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCCTGGCGCGCCGCGGATCGCCCCCGCCCCGGCCGTGGGGCTGCCGGGAACCGCCCCGGTTGCGCTGTAccc                                                          >  2:103246/1‑134 (MQ=255)
                                                                            cgcAC‑CCTGGTGCGGGGGGAACAGAGCGGGGTGGGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                         <  8:99211/135‑1 (MQ=255)
                                                                            cgcAC‑CCTGGGGCGGGGGGAACAGAGGGGCGGGCGTGCGCACTCCCCCCCGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                         <  6:347172/135‑1 (MQ=255)
                                                                             gcAA‑CCTGCGGCTGGGGGAACATAGCGGGGTGCGGGCGCACTTCAGCCATAACACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc                                                        <  1:99492/135‑1 (MQ=255)
                                                                                  ccTGCTGCTGGTGGACCAGAGCGGGGTGCGGGCGAACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                                    <  8:301322/135‑1 (MQ=255)
                                                                                                       cggggggcgggcgCACTCCCGCCAGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  7:344707/129‑1 (MQ=255)
                                                                                                       cggggTGCGTGCCCCCTCCCCCCAGACCCCCCGCGCCCGGGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  3:339434/135‑1 (MQ=255)
                                                                                                        ggcgTGCGGGCGCACTTCAGCCAGATAACCCGCGCCCGTGGCGCGCGGCTGATCCCCCCCGCCCCGGCCGTGGGGCTGCCGGTTACCGCCGCTGTTGCGCTGTACCCGCTGATACGCACGGACGCGTCGCTgcgc                              >  8:78307/1‑135 (MQ=255)
                                                                                                          cgtgcgtgCGCACTTAAGCCCGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCCGGCCCCGGCCTTGGGCCTGCCGGTTACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGCGTCGCTGCGCGa                            >  7:274939/1‑135 (MQ=255)
                                                                                                            tgggggcgcACTTCAGCCAGATCACCTGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  4:119912/130‑1 (MQ=255)
                                                                                                            tgcgtgcgCATTTCTGACAGGCCACCAGGGCCCGGGGGGGGCGGCTGATCGCCCAGGGCCAGGCCTTGGGCCGGCCGGTAAGCGCCGATGTAGCGCTGTACCAGCTGTTCGTCACGGACGAGTCGCTGCGCGAGt                          <  6:275993/135‑1 (MQ=255)
                                                                                                            ggggtgcgCACTTCAGCCAGAGCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  7:120125/132‑1 (MQ=255)
                                                                                                            ggcgtgcgCACTGCAGCCAGATCACCTGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  2:48369/134‑1 (MQ=255)
                                                                                                                tgcgCACTTCACCCAGATCCCCTGGGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtctc                      <  2:8951/135‑1 (MQ=255)
                                                                                                                   gcACTCCACCCACACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAg                   <  7:203019/135‑1 (MQ=255)
                                                                                                                       ttCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCGGCGCGAGGTCTCCAGCCt                >  6:113006/1‑134 (MQ=255)
                                                                                                                          aGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTAc            >  6:8275/1‑135 (MQ=255)
                                                                                                                           gCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTAcc           >  2:47530/1‑135 (MQ=255)
                                                                                                                                    ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  >  5:318901/1‑135 (MQ=255)
                                                                                                                                    ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCGGTACCACGTGCAgc  >  5:318904/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAA‑CCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  KT2440/5689683‑5689948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑