Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,348,4270CG23.9% 96.3 / 10.2 46M1I (ATG→ATC) †CJ019_01261hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (7/28);  new base G (11/0);  total (18/28)
Fisher's exact test for biased strand distribution p-value = 2.39e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGTAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTA  >  KT2440/1348295‑1348551
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ggTGTAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGGCGTGTCGGTTTGCAtgg                                                                                                                          <  2:171827/137‑1 (MQ=255)
  tgtAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCAtgggt                                                                                                                        <  8:336003/137‑1 (MQ=255)
   gtAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCAtgggtg                                                                                                                       <  1:126324/137‑1 (MQ=255)
           cgacAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATGCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGtctc                                                                                                               <  1:43684/137‑1 (MQ=255)
           cgacAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGtctc                                                                                                               <  4:33417/137‑1 (MQ=255)
           cgacAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGtctc                                                                                                               <  3:146794/137‑1 (MQ=255)
                   ccGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGTTTGGTTGGGTGGGGGTGTCGACAGttt                                                                                                       >  3:67343/1‑137 (MQ=255)
                         cGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTTGATGGGTGGGTGTGGGCACAGTttatttat                                                                                                 >  7:247916/1‑137 (MQ=255)
                         cGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTGCATGGGTGGGTGTCTACCCACtttgttttt                                                                                                 >  7:38719/1‑131 (MQ=255)
                         cGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGGTTGGAATGGTTGGGGTCACCAAAGTTTatatat                                                                                                 >  2:336325/1‑137 (MQ=255)
                           cTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTAGGTTTGCTTTGGTGGGAGTGTCCAAAGTTTTTATAttt                                                                                               >  2:342059/1‑136 (MQ=255)
                              gATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTGCACCGTTTCTATATTGtta                                                                                            >  8:348901/1‑136 (MQ=255)
                              gATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTc                                                                                            <  1:228462/137‑1 (MQ=255)
                                  aCGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTTGATGGGTGGGGGTGTCCACAGTTTGTATATTGttgcggg                                                                                        >  5:353637/1‑132 (MQ=255)
                                      gcAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGCTGGGGTTGGGGGGGTGTGTGCACCGGTTCTCTTTTGTTCAGGCGccg                                                                                    >  7:20853/1‑136 (MQ=255)
                                          ttGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATg                                                                                <  7:237714/137‑1 (MQ=255)
                                          ttGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATg                                                                                <  3:140586/137‑1 (MQ=255)
                                          ttGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATg                                                                                <  2:222070/137‑1 (MQ=255)
                                          tgggCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAAGCGGCGGCCTGCGGCACCTGGGCCTGGGGGTTCTCGCGAGGCGTGTCGGTTTGCATGGGTGGGGGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATg                                                                                <  6:15502/135‑1 (MQ=255)
                                           tGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGGTTGGATGGGTGGGTGTGGCCACAGTTTGTTTATTTTTACGGCGCCTTATgg                                                                               >  5:242616/1‑137 (MQ=255)
                                           tGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGGTTGCCTGGGGGGGGGGGGGCACCGTTTATATATTTTTTAGGGGCCGTAttt                                                                               >  2:229958/1‑135 (MQ=255)
                                             gCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACGTGGGCCTGCGGGTTCTCTCTAGTCGTTTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGgc                                                                             <  8:89978/137‑1 (MQ=255)
                                                  gggCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGGATGGGTGGGTGTGTGCCCAGTTTCTATATTTGTCATGCGGCTTTTGGGCGcccc                                                                        >  7:294249/1‑136 (MQ=255)
                                                     cGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTGGATGGGTGGGTGTCTCCACAGTTTCTATATTTTTAAGGCGGCTTATGGGGGccccccc                                                                     >  5:116084/1‑136 (MQ=255)
                                                     cGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGGATTGGTGGGTGTGTGCCCAGTTTGTTTATTTTTGAGGCGCCAGATGGGGGGCCAccc                                                                     >  3:89567/1‑136 (MQ=255)
                                                     cGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGGTTGCATGGGGGGGGGTGTCCCCAGGTTGTATATTGTTGAGGCGCCTTATGGGGGCCCCCCg                                                                     >  5:77139/1‑137 (MQ=255)
                                                       gTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGGATGGGTGGGTGTGGCCACAGGTTGTATATTGTTAAGGGGGCGTTTGGGGGCCCCCCggt                                                                   >  4:318509/1‑135 (MQ=255)
                                                               cAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCAc                                                           <  2:86866/137‑1 (MQ=255)
                                                               cAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTACATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCAc                                                           <  8:322941/137‑1 (MQ=255)
                                                                 gATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGTGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACtt                                                         <  7:171833/137‑1 (MQ=255)
                                                                           ggAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTGCATGGGTGGGTGTGTCCACAGTTTCTATATTTTTCAGGCGCCGTATGGGCGCCCACCGGTCCGTGCACTTGCTCGATaaa                                               >  8:92841/1‑136 (MQ=255)
                                                                            gAATCTGCGGCCTGCGGCACCTGGGCCTGCGGGTTCGCGCGAGTCGTGTCGGTTTGCATGGGTGGGAGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAAcc                                              <  8:184351/137‑1 (MQ=255)
                                                                            gAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAAcc                                              <  4:144172/137‑1 (MQ=255)
                                                                            gAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAAcc                                              <  6:307404/137‑1 (MQ=255)
                                                                            gAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAAcc                                              <  7:55472/137‑1 (MQ=255)
                                                                                      ccTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGAt                                    <  7:96850/137‑1 (MQ=255)
                                                                                      ccTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGAt                                    <  3:335334/137‑1 (MQ=255)
                                                                                              agcTGGGCCTGCGGGTTCTCGCGAGGCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCGTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAatg                            <  7:172743/135‑1 (MQ=255)
                                                                                              aCCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAatg                            <  2:311390/137‑1 (MQ=255)
                                                                                              aCCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAatg                            <  7:172747/137‑1 (MQ=255)
                                                                                                  gggCCTGCGGGTTCTCGCGAGTCGTGGCGGTTTGCATGGGTGGGTGTCTCCACAGTTTGTATATTTTTAAGGCGCCTTATTGGGGCCCCCCGTTCCGTCCCATTTGTCGATAAACAAGCCGGGATGCGCAatgcata                        >  2:169972/1‑136 (MQ=255)
                                                                                                             ttCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGGGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATccgccc             <  4:67343/137‑1 (MQ=255)
                                                                                                             ttCTCGCGAGTCGTGGCGGTTTGCATGGGTGGGTGGCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATccgccc             <  8:287208/137‑1 (MQ=255)
                                                                                                               ctcGCGAGTCGTGTCGGTTTGCATGGGTGGGTGGCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATccgcccgc           <  7:348901/137‑1 (MQ=255)
                                                                                                                       gcGTGGCGGTTTGCCGGGGTGGGTGTCCCCACAGTTTCTATATTGTTCAGGCGCCTTCTGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGt   <  4:234559/136‑1 (MQ=255)
                                                                                                                        cGTGTCGGTTTGGATGGGTGGGTGTGTCCACAGTTTGTATATTGTTAAGGCGCCTTATGGGCGCCCCCCCTTCCGGCCACTTTCTCGATAAACACCCGGGGATTCGCAAATCATGCGCATCCCCCCCCCCGGGCgga  >  2:19078/1‑135 (MQ=255)
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GGTGTAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCTCCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCACCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTA  >  KT2440/1348295‑1348551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑