Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,669,1300GT28.8% 60.6 / 11.4 52L161L (CTC→CTA) chrRQuinone reductase
Reads supporting (aligned to +/- strand):  ref base G (23/14);  new base T (14/1);  total (37/15)
Fisher's exact test for biased strand distribution p-value = 4.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCGGCG  >  KT2440/4669011‑4669266
                                                                                                                       |                                                                                                                                        
cccGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGGCATTGAGCTTGCCCGAATCCTc                                                                                                                         >  2:54303/1‑137 (MQ=255)
        tACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGc                                                                                                                 >  5:150064/1‑137 (MQ=255)
                ggCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCa                                                                                                         >  8:3768/1‑137 (MQ=255)
                ggCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCa                                                                                                         >  8:265591/1‑137 (MQ=255)
                 gCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAc                                                                                                        <  6:351311/137‑1 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGCCCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCTTGTGATTTAGCTTGCCCGCATCCGCGAACAAGCTCGCCGcccc                                                                                                       >  1:304344/1‑137 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                       >  6:325577/1‑137 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                       <  7:265591/137‑1 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAAAAAGCTCGCCGcccc                                                                                                       >  3:147004/1‑137 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTAGTGTCATTGAGCTTGACCGAAACCTCGAAAAAGCACCCCGCAcc                                                                                                       >  4:41105/1‑137 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGGTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCAcc                                                                                                       >  4:95924/1‑137 (MQ=255)
                  cGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGGGTGTCATTTAGCTTGGCCCCATCCTCGAACAACCTcgccccccc                                                                                                       >  1:338292/1‑132 (MQ=255)
                       cacaGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGGCGTGTGATTTAGCTTGCCCGCATACTAGCACAAGCGCGCCGCCCCCCCaa                                                                                                  >  1:77337/1‑137 (MQ=255)
                                 ttAGGGGGTCAGACCGCCCTGTTCAACTTCACCCATCACGCAAACCTGACGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTc                                                                                        <  1:141640/137‑1 (MQ=255)
                                  tAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCg                                                                                       >  6:261312/1‑137 (MQ=255)
                                         tCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGCTCGCGGGATGTCATTTAGCATTGCCGAATACTGGAAAAAGCTCGCCGCCCCCCCAAGGGAAGGCTCGGGCCTcc                                                                                >  2:127552/1‑136 (MQ=255)
                                           aGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGAGTCTTGTCATTTAGCTTGCACGAATCCTACAACAAGCACGCCGCACACCCAAGGTAAGCCGCGGGCATCtgc                                                                              >  7:272702/1‑137 (MQ=255)
                                            gACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCtgca                                                                             >  4:127181/1‑137 (MQ=255)
                                            gACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCtgca                                                                             >  5:138109/1‑137 (MQ=255)
                                            gACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTAGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCATGCCCGGAACCTAGAACAAGCTAGCCCCACCGCAAAGGTAAGCCTCGGGCATCtgca                                                                             >  7:306524/1‑137 (MQ=255)
                                             aCCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTGATTTAGCTTGCCCGCATACTCGAACCACCTCGCCCCCCCCCCCACGGAAGCCACGGGCCTcatcaa                                                                            >  1:299662/1‑132 (MQ=255)
                                             aCCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCACGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCtccat                                                                            >  5:266569/1‑137 (MQ=255)
                                              ccGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGATCTTTCCCCAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCtgcatg                                                                           >  6:230150/1‑137 (MQ=255)
                                                gCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCtgcatgca                                                                         <  6:138109/137‑1 (MQ=255)
                                                         aaCTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCCCCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTc                                                                >  4:159848/1‑137 (MQ=255)
                                                         aaCTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTc                                                                >  2:5345/1‑137 (MQ=255)
                                                         aaCTTCACCCATGACGCAAACCTGTCGACAAACGCCAGCAAGAACGGTCGCGTCATGTCATTTATCATGACCGAAACCGCCAACAACCTAGCCCCCCCGCCAAGCTAAGCCACGGGCCTCTGCAAGCACGGCAAGtg                                                                >  2:324934/1‑136 (MQ=255)
                                                          aCTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCg                                                               <  7:3768/137‑1 (MQ=255)
                                                              cACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGaa                                                           >  4:101549/1‑137 (MQ=255)
                                                                 ccATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAaca                                                        >  4:308880/1‑137 (MQ=255)
                                                                 ccATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAaca                                                        >  7:52874/1‑137 (MQ=255)
                                                                   aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACAcc                                                      >  5:326557/1‑137 (MQ=255)
                                                                   aTGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCAAGCAGGGCATGTCGAGAAACAcc                                                      >  3:135363/1‑137 (MQ=255)
                                                                        gCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTATCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATTCAGGGCATTTCGAGAAACACCAGCgc                                                 >  2:154344/1‑136 (MQ=255)
                                                                             ccTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGgc                                            <  5:290300/137‑1 (MQ=255)
                                                                                  cGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTc                                                                                        >  8:5610/1‑88 (MQ=255)
                                                                                  cGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTc                                                                                        <  7:5610/88‑1 (MQ=255)
                                                                                  cGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATACTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCAcc                                       >  8:333443/1‑137 (MQ=255)
                                                                                    aCAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGCGCTTGCCCGAATACGCGAACAAGCTCGCCGCACCGCCCAGGTAAGCCTCGGGCATCTGCATTCAGGGCATGGCGAGCACCCCCAGCGACTGGCGCCCCGc                                     >  8:281880/1‑137 (MQ=255)
                                                                                    aCAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGc                                     >  7:202406/1‑137 (MQ=255)
                                                                                             tGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTaa                                                                                             >  5:264652/1‑72 (MQ=255)
                                                                                             tGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTaa                                                                                             <  6:264652/72‑1 (MQ=255)
                                                                                                   aaCGGTCGCGTCTTGGCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCaaa                      <  8:73367/137‑1 (MQ=255)
                                                                                                       gTCGCGTGTTGTCATTGAGCTTGCCCGAATCCGCGAACAAGCTCGCCGCACCGCCAAGGGAAGCCTCGGGCATCTGCCTGCAGGGCATGTCGCGAAACACCAGCGACTGGCGCCCCGCATGGTTGGCGCCAAAGccc                  >  6:219569/1‑136 (MQ=255)
                                                                                                       gTCGCGTCTTGTCATTGAGCTTGCCCGCATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCCCCGCATGGTTGGCGCCAAAGccc                  >  4:172285/1‑136 (MQ=255)
                                                                                                         cgcgTCTTGTCATTTAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGCGAAACACCCGCGACTGGCGCACCGCATGGTTGGCGCCAAACccacc                >  4:279896/1‑137 (MQ=255)
                                                                                                         cgcgTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGccacc                <  2:77337/137‑1 (MQ=255)
                                                                                                               ttGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGc          <  3:269392/137‑1 (MQ=255)
                                                                                                                    aTTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCg     <  2:299662/137‑1 (MQ=255)
                                                                                                                    aTTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCg     <  6:150064/137‑1 (MQ=255)
                                                                                                                      tGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCGGc   <  2:338292/137‑1 (MQ=255)
                                                                                                                      tGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCGGc   <  2:238166/137‑1 (MQ=255)
                                                                                                                       gAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCTACCAATCGCCCCCGGCg  <  2:304344/137‑1 (MQ=255)
                                                                                                                       |                                                                                                                                        
CCCGCTCCTACACAGGGGCGTTGCACAGCAGGATTAGGGGTTCAGACCGCCCTGTTCAACTTCACCCATGACGCAAACCTGTCGACAAACGCCTGCAAGAACGGTCGCGTCTTGTCATTGAGCTTGCCCGAATCCTCGAACAAGCTCGCCGCACCGCCAAGGTAAGCCTCGGGCATCTGCATGCAGGGCATGTCGAGAAACACCAGCGACTGGCGCACCGCATGGTTGGCGCCAAAGCCACCAATCGCCCCCGGCG  >  KT2440/4669011‑4669266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑