Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 2,266,996 T→G 22.9% N143T (AAC→ACC)  CJ019_02153 ← putative HTH‑type transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,266,9960TG22.9% 84.6 / 3.6 54N143T (AAC→ACC) CJ019_02153putative HTH‑type transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base G (12/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCGGCCTCGC  >  KT2440/2266866‑2267128
                                                                                                                                  |                                                                                                                                    
cGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCCGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGGTGTa                                                                                                                                  >  1:53467/1‑135 (MQ=255)
       tAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGtt                                                                                                                            <  7:62144/134‑1 (MQ=255)
             gcgGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCCGCCCGCCGGCCAGCAGTGCGTCGACCACAAAGCGGGTGTAGTGGTTGGAgggg                                                                                                                     >  3:94793/1‑135 (MQ=255)
               ggCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCCACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGCCCACAAAGCGGTTGGAGTCGTTGTTgggggt                                                                                                                   >  3:225911/1‑133 (MQ=255)
                 cATAGGAGGGCGCGGCAAACGCGATCGCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCGGGCCAGCAGTGAGTCGACCACAACGCGGTTGTAGTCGTTGTAGGGGCTGa                                                                                                                 <  8:30814/135‑1 (MQ=255)
                         ggCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGGCGGCCAGCTCGGCCCACGGGGCCCGCCCGCCGGCCCGCAGGGAGTCGACCCCAAATCGGTTGTTGTCGTTGTTGGGGGTGGTGGtata                                                                                                          >  6:206949/1‑134 (MQ=255)
                           cgcgGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGaa                                                                                                       <  7:36333/135‑1 (MQ=255)
                            gcgGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCCGCCCGCCGGCCCGCAGTGAGTCGCCCACAAAGCGGGTGTAGTCGTTGTAGGGGCGGATGGAATAGAAg                                                                                                      >  2:234258/1‑135 (MQ=255)
                            gcgGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCCGCCGGCCAGCAGTGAGTCGACCACAAAGCGGGTGTAGTCGTTGTAGGGGCTGATGGAATAGAAg                                                                                                      >  3:309263/1‑135 (MQ=255)
                              ggCAACCTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCCGCCAGCTCCGCCACCTGGGCCCGCCAGCCGGGCAGCCGGGAGTCGACCACAAACCGGGTGTAGGCGTTGTAGGGGCGGATGGCTTAGAAgcg                                                                                                    >  7:74391/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCCGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGCCCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcg                                                                                                    >  1:15053/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCCGCTCCGCCCACTGGGCCCGCCCCCCGGCCCCCAGTGCGTCGACCACCAAGCGGGTGTAGTCGGTGGTGGGGCGGATGGAATAGAAgcg                                                                                                    >  1:88948/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCCCCGCGGCCCCCAGGGCCCCCCCCCCGGCCCCCCGGGAGTCGCCCCCAAAGCGGGTGTAGTCGGTGGTGGGGCGGCTGGGATAGAAgcg                                                                                                    >  5:101648/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCCACAGGGCCCGCCAGCCGGCCCGCAGTGAGTCGACCACAAACCGGTTGTAGGCGTTGTATGGGCGGATGGGATAGAAgcg                                                                                                    >  7:310288/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCCACAGGGCCCGCCAGCCCGCCAGCAGTGAGTCGACCACAAAGCGGGTGTAGTCGGTGTAGGGGGGGTTGGAATATAAgcg                                                                                                    >  3:130514/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGTCAGCCCGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGGTGTAGGGGCTGAAGGAATAgcaccg                                                                                                    >  5:307975/1‑130 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCCGCCGGCCAGCAGTGAGTCGACCACAAAGCGGGTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcg                                                                                                    >  3:135353/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCCCAAAGCGGGTTTTGTCGTTTTTGGGGCTGATGGAATAGAAgcg                                                                                                    >  7:37736/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcg                                                                                                    >  5:321705/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGGTGTAGGGGGTGATGGTATAGAAgc                                                                                                     >  4:146396/1‑134 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAACCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcg                                                                                                    >  8:269748/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACAGGGCCAGCCAGCCGGCCAGCAGTGCGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcg                                                                                                    >  1:240326/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCCGCCCACTGGGCCAGCCAGCCCGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGGCCTTGTTGGGGCGGATGGAATATAAgcg                                                                                                    >  3:311263/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTAGGCCAACTGGGCCAGCCCGCCGGCCAGCCGGGAGTCGACCACAAAGCGGGTGTAGTCGTTGTAGGGGGGGATGGAATATAAgcg                                                                                                    >  3:149362/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCACGGCCAACTTGGCCACCCAGCCCGCCAGGAGTGAGTGGACCCCAAACCGGTTGTAGTCCTTCTAGGGGGTGATGGAATATAAccg                                                                                                    >  2:207854/1‑135 (MQ=255)
                              ggCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCCGCCCGCTCGGCCAACTGGGCCCGCCCGCCCGCCAGCAGTGAGTCGACCCCAAAGCGGGTGTATTCGTTGTAGGGGGGGCTGGAATAGAAgcg                                                                                                    >  5:99580/1‑135 (MQ=255)
                                cAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCCGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCGGGGGGAATAGAAGCCCa                                                                                                  >  1:327644/1‑135 (MQ=255)
                                  aaCTCGAGCTCCAGGCGTGCGGCCGGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGc                                                                                                <  7:146098/135‑1 (MQ=255)
                                           tcCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGccc                                                                                       <  8:309786/135‑1 (MQ=255)
                                              aGGCGGGCGGCCTGCACCGGGGGGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGGCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGgcg                                                                                          <  5:224466/129‑1 (MQ=255)
                                              aGGCGATCGGCCTGCACCGGGGGGCCGGCCAGCGCGGCCAACTGGCCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGgcg                                                                                          <  5:202014/129‑1 (MQ=255)
                                                gCGTTCGGCCTGCACCGGGGTGCCGGCCAGCGCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGGAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGc                                                                                  <  3:98804/135‑1 (MQ=255)
                                                     cGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCtt                                                                             <  5:229423/135‑1 (MQ=255)
                                                      ggCCTGCACCGGGGTGCCGGCCCCCTCGGCCCACTGGGCCCGCCCGCCGGCCAGCAGTGAGTCGACCACAAACCGGTTGTAGTCGGTGTAGGGGCGGATGGAATAGAAGCGCCGCCCGGCCCCCGGGGCATCGTc                                                                            >  2:60232/1‑135 (MQ=255)
                                                          tGCACCGTGGTGCCGGCCAGCTCTGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATTGAATAGAAGCGCAGCCAGGTGCCCTGGGCATCTTCCTCa                                                                        <  6:219647/135‑1 (MQ=255)
                                                          tGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGt                                                                                                                                      >  8:186849/1‑73 (MQ=255)
                                                          tGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGt                                                                                                                                      <  7:186849/73‑1 (MQ=255)
                                                                    ggCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAACGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAAt                                                              <  6:101648/134‑1 (MQ=255)
                                                                      ccGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACACAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATgg                                                            <  4:19610/135‑1 (MQ=255)
                                                                      ccGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATgg                                                            <  6:88135/135‑1 (MQ=255)
                                                                               tCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGGTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCGTCCTCAAAGCGGGAATGGCCGCGGTAg                                                   >  4:45847/1‑135 (MQ=255)
                                                                                 ggCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGtt                                                 >  4:19641/1‑135 (MQ=255)
                                                                                                 cagccGGCCAGCAGTGAGGCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGc                                 <  5:206949/135‑1 (MQ=255)
                                                                                                  agccGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCt                                >  7:89707/1‑135 (MQ=255)
                                                                                                         ctgaagTGCGGCGACCACAAAGCGGTTGTAGTCGTTGTTGGGGCTGATGCAATAGACGCGCAGCCCGGCGCCCTGGGCATCTTCCTCAACGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACg                         <  2:327644/131‑1 (MQ=255)
                                                                                                         cagcagTGAGTCTACCACAACGCGGTTGTTGTCGTTGTAGGGGCTGATGTAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGTAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACg                         <  6:307975/135‑1 (MQ=255)
                                                                                                         cagcagTGAGTCGACCACAAAGCGGGTGTAGTCGTTGTAGGGGCTGAGGGAATCGACGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAACGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACg                         <  5:121986/135‑1 (MQ=255)
                                                                                                         cagcagGGAGTCGACCACAAAGCGGTTGTTGGCTTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACg                         <  2:125067/135‑1 (MQ=255)
                                                                                                         cagcagGGAGGCGACCACAAAGCGGGTGTAGTCGTTGTAGGGGCTGATGGAATAGACGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACg                         <  5:299866/135‑1 (MQ=255)
                                                                                                                         acaAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCATCAGGGTAcgcgcg         >  8:157919/1‑135 (MQ=255)
                                                                                                                         acaAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTAcgcgcg         >  8:3433/1‑135 (MQ=255)
                                                                                                                          caAAGCGGTTGTAGTCGTTGTAGGGGCGGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCgg        <  2:240326/135‑1 (MQ=255)
                                                                                                                              gCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCGGCCTc    <  2:253617/135‑1 (MQ=255)
                                                                                                                              gCGGTTGTAGGCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCGGCCTc    <  4:149362/135‑1 (MQ=255)
                                                                                                                              gCGGTTGTAGGCGTTGTAGCGGCTGATGGACTAGACGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAACGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCGGCCTc    <  7:5258/135‑1 (MQ=255)
                                                                                                                                ggTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCGGCCTCGc  >  5:73656/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGCCGCGTAAAGCGGCTCGAAACGCAGCAGGGTACGCGCGGCCTCGC  >  KT2440/2266866‑2267128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑