Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	2,910,766	A→C	20.6%	K2243N (AAA→AAC)	CJ019_02759 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,910,766	0	A	C	20.6%	101.2 / 4.4	55	K2243N (AAA→AAC)	CJ019_02759	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/17);  new base C (2/11);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 9.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCAGCTCAAGCCGTACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGGTGTACTGGGTACTGCC  >  KT2440/2910635‑2910895                                                                                                                                    |                                                                                                                                   aCCCAGCTCACGCGGTACACCTGGTGGGTGGACGTGGTGCAACACCTGACGCACCATTTGTCGCTTATCAACTTCATGGCGGCCTACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGTAAAAAcgc                                                                                                                                 <  5:104380/135‑1 (MQ=25)  cccAGCGCACGCCGTCCACCAGCTGGGTCGACCTGGGGCAACACCTGACGCACCCAGAGGCGCTGATCAACTTCAGCGCGGCCTACGGCACCCACAGCACCATCCCCAATGCCACCCCCCTGTTGGAAAACcgcg                                                                                                                                <  5:72555/135‑1 (MQ=37)      gCTCAAGCCGTACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCGGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGcgccg‑c                                                                                                                           <  5:35275/135‑1 (MQ=255)        tCACGCCGTACCCCCGCTGGGTCGACCGGGTGCAACACCTGACGCACCCAGAGGCGCTGATCAACTTCATCGCGGCCTACGGCACCCACACCCCCATCACCAATCCCACCACCCTGTTGGAAAACCGCGCCG‑Cgg                                                                                                                         <  2:30032/135‑1 (MQ=255)        acAACCCGTCCACCAGTGGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCAACTTCATCGGGGCCTACGCAACCCACACCACCATCACAAATGCCCCCACCCTGTTGGAAAACCGCGCCG‑Cgg                                                                                                                         <  2:75846/134‑1 (MQ=255)         cAAGCCGTACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCCCCACTGCCCCCACCCTGTTGGAAAAACGCGCCG‑CGGc                                                                                                                        >  3:298124/1‑135 (MQ=255)          aaGCCGTACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCCACTTCATCGCGGCCTACGGCACCCCCAGCACCATCACCAATGCCACCACCCTGGTGGAAAAACGCGCCG‑Cgg                                                                                                                         >  4:229285/1‑133 (MQ=255)          aaGCCGTACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCGGATCAACATCATCGCGGCCGACGGCCCCCACAGCCCCAACACCAATGCCCCCACCCTCGTGGTAAAACCcgcccccgc                                                                                                                         >  1:304557/1‑129 (MQ=255)              cttACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCAACTTCAACGCGGCCTACGGCACCCACACCACCATCACCAATGCCACCCCCCTGTTGCAAAAACGCGCCG‑CGGCCATgg                                                                                                                   <  6:245115/133‑1 (MQ=38)                    ccAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGCTCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCACCAATGCCAACACCCTGGTGGCTAAACGCGCCG‑CGGCCATGGCCCTgg                                                                                                             >  6:78024/1‑135 (MQ=37)                          gggTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCAACTTAATAGCGGCCTACGGCACCCACAGCCCCATCACCCATGCCCCCCCCCTGTTTGTAAACCCCCCCC‑CGGCCCAGGCCCGGGTGttgg                                                                                                       >  5:114475/1‑133 (MQ=21)                          gggTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCGGAGCAACATGAAAGCGGCCTAACGCCCCCCCCGCACCCACCCCCAAGCCACCCCCCCCTTGGGAAAACCCGCCG‑CGGCCACTGCCCGGGGGTTCg                                                                                                       >  2:327634/1‑135 (MQ=255)                           ggTCGACCTGGTGCAACACCTGAAGAACCCAGAGTCGCTGATCAACTTCATAGCGGCCTAACGCAACCACAGCACGAACACCAAAGCCCCCCACCTGTTGGAAAAAAGCGACG‑CCGCCAAGGCCCAGGTGttacg                                                                                                      >  6:324942/1‑132 (MQ=255)                                            acCTGAAGCACCCAGAGGCGCTGAGCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCACCAATCCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACccg                                                                                     <  4:37577/135‑1 (MQ=255)                                              cTGAAGCACCCAGAGTCGCGGATGAACTTCATCGCGGCCGACGGGACCCACCCCCCCCACACCCAAGCCCCCACCCGGTTGGTAAAACGCGCCC‑CGGCCCTGGCCCGGGTGTTGGGTGGCGCGGGCGcccccccc                                                                                   >  6:241734/1‑135 (MQ=255)                                                  aGCACCCAGAGTCGCTGATCAACTTCATAGCGGCCTACGGGACCCACACCACCAACACCAATGCCCCCACCCAGTTTGAAAAACCCGCCG‑CGGCCCTGGCCCTGGTGTTCGGTGGCGATGGCGCACCCCCCGccc                                                                               >  7:91721/1‑135 (MQ=255)                                                   gCACCCAGAGTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCCCCATCCCCAAAGCCACCCCCCTGTTGGAAAAACCCGCCG‑CGGCCCAGGCCCTGGTGTTAGGTGGCGATGGCGCACCCGCCGCCCg                                                                              >  3:221136/1‑135 (MQ=37)                                                   gCACCCAGAGTCGCTGATCAACTTCATAGCGGCCAACGGCACCCACAGCCCCCTCACCAATGCCCCCCCCCTGTTGGAAAAACCCGCCC‑CGGCCATGGCCCGGGTGTTCGGTGGCGATGGGGCACCCGCCGACCg                                                                              >  2:278269/1‑135 (MQ=37)                                                   gCACCCAGAGTCGCTGATAAACGTAAAAGCCGCCTAACGCACCCACAGCCCAATAACCCAAGCCCCCCCCCCGGTGTAAAACACCGCCGCCGCCCATGG‑CCTGGTGTTCCGTGGGGAAGGCGCCCCCcccgcccg                                                                              >  4:191868/1‑135 (MQ=255)                                                       ccAGAGTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATg                                                                          <  8:91721/135‑1 (MQ=255)                                                       ccAGAGTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCACCACCAATGCCACCACCCTGTTGAAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATg                                                                          <  3:310736/135‑1 (MQ=255)                                                         agagTCGCTGCTCAACTTCATCGCGGCCTACGGCACCCACAGCCCCATCACCAATGCCACCACCCGGTTGGCAAAACGCGCCG‑CGGCCATGGCCCTGGGGTTCGGTGGCGATGGCGCCCCCGCCGCCCGCATggc                                                                        >  4:158260/1‑134 (MQ=255)                                                         agagTCGCTGATCAACTTCATCGCGGCCTACGGCCCCCACACCACCATACCCAAAGCCACCACCCTGTTGGAAAAACCCGCCC‑CGGCCAAGGCCCGGGGGTTCGGTGGCGATGGCGCACCCGCCGCCCCCATGGa                                                                        >  6:133598/1‑135 (MQ=255)                                                          gagTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCAACACCAATGCCACCCCCCTGTTGGAAAAACCCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCCATGGCGCCCCCCCCGACCGCCAGGAc                                                                       >  7:113737/1‑135 (MQ=38)                                                           agTCGCTGATCAACTTCATCGCGGCCTACGGCCCCCACAGCACCATCACCAATGCCCCCACCCAGGTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTAGGTGGCGATTGCGCACCCGccgcccgccaggcc                                                                       >  6:65191/1‑128 (MQ=255)                                                                   aTCAACTTCATCGCGGCCTAAGGCACCCACAGCACCATCACCAATGCCACCACCCAGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCCCCCGCCGCCCGCATGGACGTCCTCa                                                                >  6:121459/1‑133 (MQ=255)                                                                     cAACTTCATCGCGGCCTACGGCACCCACACCACCATCCCCAATCCCACCACCCTGTGGCAAACACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAAcag                                                            <  2:59469/135‑1 (MQ=255)                                                                                cgGCCTACGGCACCCACAGCACCATCACCAATGCGACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCt                                                 >  1:140004/1‑135 (MQ=255)                                                                                cgGCCTACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACTCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGAATGGACTTCCTCAACAGCAGCGGCGCCt                                                 >  2:145301/1‑135 (MQ=255)                                                                                 ggCCTACGGCACCCACAGCACCATCACCAATCCCACCACCCTTTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTg                                                <  5:65191/135‑1 (MQ=255)                                                                                     tACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGc                                             >  1:97250/1‑134 (MQ=255)                                                                                           aCCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTg                                      <  4:157950/135‑1 (MQ=255)                                                                                           aCCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTg                                      <  8:256277/135‑1 (MQ=255)                                                                                              cacaGCACCATCACCAATCCCACCACCCTGTGGTAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGAcc                                   <  2:279447/135‑1 (MQ=255)                                                                                                  gCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGc                               >  5:61349/1‑135 (MQ=255)                                                                                                      caccACCAATGCCACCACCCTGTTGTAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGGAGCGGCGCCTGGGCCAACGTGACCCTGCCCgg                           <  8:154542/132‑1 (MQ=255)                                                                                                      caccACCAAGCCCACCACCCTGTTGTAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCgg                           <  5:133598/132‑1 (MQ=255)                                                                                                      cATCACCATGGCCACACCCGTGTTGTAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCgg                           <  2:97250/135‑1 (MQ=255)                                                                                                       aTCACCAATGCCACAACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCgg                           >  3:111841/1‑134 (MQ=255)                                                                                                         cACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGGTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCaa                        <  8:267192/135‑1 (MQ=255)                                                                                                         cACCAATGCCACCACACTGTTGGAAAAACGCGACG‑CGGCCATGGCCCTGGTGTTCGGTGGCGCTGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCCACGTGACCCTGCCCGGCaa                        >  7:222187/1‑135 (MQ=255)                                                                                                           ccAATGCCACCACCCTGTTGGAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAgg                      <  4:221136/135‑1 (MQ=255)                                                                                                           ccAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAgg                      >  7:28419/1‑135 (MQ=255)                                                                                                           ccAATGACACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATCGACTTCCTCAACAGCAGCGGCGCCAGCGCCAACGAGACCCTGCCCGGCAAgg                      >  1:131177/1‑135 (MQ=255)                                                                                                           ccAAGGCCACCACCCTGTTGGAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAgg                      <  3:229285/135‑1 (MQ=255)                                                                                                           caaaTGCCACCACCCTGTTGTAAACCCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAgg                      <  2:272659/133‑1 (MQ=255)                                                                                                                gCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGgt                 <  6:131818/135‑1 (MQ=255)                                                                                                                gCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGgt                 <  8:28419/135‑1 (MQ=255)                                                                                                                  ccccaccGTGTTGCAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGgtgt               <  5:131893/133‑1 (MQ=255)                                                                                                                  caccacCGTGTTCTAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGgtgt               <  6:237279/135‑1 (MQ=255)                                                                                                                     caccCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGGTGTACt            <  6:61349/135‑1 (MQ=255)                                                                                                                       cccTGTTGGAAACACGCGCCG‑CGGCCATGGCCCTGGGGTGCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGGTGTACTgg          <  2:193482/135‑1 (MQ=255)                                                                                                                             tGGAAAACCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAgcggc                                                     <  5:272549/86‑1 (MQ=255)                                                                                                                             tGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAgcggc                                                     >  6:272549/1‑86 (MQ=255)                                                                                                                              ggAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGGTGTACTGGGTACTGc   >  3:332270/1‑135 (MQ=255)                                                                                                                               caaacaCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGGTGTACTGGGTACTGcc  <  1:278269/134‑1 (MQ=255)                                                                                                                                    aCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGgt                 >  3:150852/1‑115 (MQ=255)                                                                                                                                    aCGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGgt                 <  4:150852/115‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   ACCCAGCTCAAGCCGTACACCAGCTGGGTCGACCTGGTGCAACACCTGAAGCACCCAGAGTCGCTGATCAACTTCATCGCGGCCTACGGCACCCACAGCACCATCACCAATGCCACCACCCTGTTGGAAAAACGCGCCG‑CGGCCATGGCCCTGGTGTTCGGTGGCGATGGCGCACCCGCCGACCGCATGGACTTCCTCAACAGCAGCGGCGCCTGGGCCAACGTGACCCTGCCCGGCAAGGACGGTGTACTGGGTACTGCC  >  KT2440/2910635‑2910895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑