Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,245,154	T→G	38.8%	D175A (GAT→GCT)	CJ019_03982 ←	Crotonyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,245,154	0	T	G	38.8%	33.4 / 9.5	53	D175A (GAT→GCT)	CJ019_03982	Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base T (17/14);  new base G (3/19);  total (20/33)
Fisher's exact test for biased strand distribution p-value = 3.59e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAG  >  KT2440/4245020‑4245279                                                                                                                                         |                                                                                                                              tgcgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGAt                                                                                                                               <  1:45094/134‑1 (MQ=255)             gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCCGGCGCGCGCGGCGccccccc                                                                                                                   >  1:245988/1‑132 (MQ=255)                 ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCCGGCGCGAGCGGCGCCCCCCCACtc                                                                                                               >  3:154195/1‑135 (MQ=255)                 ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑CG‑CCCGGGCGCGATCGGCGCCCCACAAcac                                                                                                               >  8:284132/1‑133 (MQ=255)                   tCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGC‑GG‑CCCGGGCGCGCTCGGCGCCCCCCCACtcgt                                                                                                             >  4:23577/1‑135 (MQ=255)                   gcTGCGCATCGGCGAAGGGCGGGCCATTGGCCCGGCGCCCGGCCAGTTGGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCATACTTCGCTGACAAACCCGGC‑GG‑CAAGGGCGCTATCGGCGCCCCACAACtcg                                                                                                              <  7:19880/133‑1 (MQ=255)                          aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCGGGCGCGATCGGCGCCCCACAACGCGTCGAggc                                                                                                       >  4:115501/1‑133 (MQ=255)                            cggCGAAGGGCGGGCCTTGGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACGGCGCGGCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                    <  6:338742/134‑1 (MQ=255)                            aGGGGATGGGCGGGCCTTTGGCCAGCGGCCGGGCCATTTGGCCGGCAACGGCAGGAAACTGCTCGTCGGCGAATTATTCGCTGACAAACCGTGC‑GG‑CAAGGGCGCTATCGGCGCCCCACAACTCGGCGAGGAAca                                                                                                    <  6:82521/135‑1 (MQ=255)                            aGGCGACGGGCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTCACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                    <  8:251743/135‑1 (MQ=255)                               cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCACGGCGCGATGGGCGCCCCACAACACGTCGAGGAAcatca                                                                                                 >  5:146456/1‑135 (MQ=255)                                gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGCAcagcag                                                                                                >  4:201418/1‑135 (MQ=255)                                 agggcggggCCTTGGCCCGGGCGCCGGGCCTGTGGGGCGCCACGGGCAGGCACCTGGGGGGCGGCGCAGACTGCGCTCACCACCCCGGC‑GG‑CCAGGGCGCGAGCGCCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                               <  2:332492/129‑1 (MQ=255)                                    gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTAGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCGGGCGCGATCGGCGCCCCCCAACGCGGCGCGGGACAGCAGgcgc                                                                                            >  2:2528/1‑135 (MQ=255)                                    gTCGGGCCATTGGACAGGCGACCGGCCAGTTCGGCGGCCACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACGACCCCGGC‑GG‑CCCCGGCGCGCTCCGCGGCCCACAAATCGTCGAGGCACAGCCGgcgc                                                                                            >  7:93666/1‑135 (MQ=255)                                           cATTGGCCCGGCGCCCGGCCAGTGCGGCGGCACCGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGC‑GG‑CCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCt                                                                                     <  4:276196/135‑1 (MQ=255)                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCACCGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGCCCAACCCGGC‑GG‑CCCGGGCGCGATCGGCGCCCCCCAACGCGTCGAGGCACAGCAGGCGCGTGGCCt                                                                                     >  3:150874/1‑135 (MQ=255)                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCTGGCGCGCTCGGGGCCCCCCAACGCGTCGGGGGACCCCAGGCGCGTgggcg                                                                                     >  5:301972/1‑132 (MQ=255)                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCGGGCGCGCTCGGCGCCCCCCAACGCGTGGCGGGACAGCAGGCGCGTGGccg                                                                                     >  3:195400/1‑134 (MQ=255)                                           cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCGGGCGCGCTCGGCGCCCCCCAACACGGCGCGGAACAGCCCGCCCATGGcca                                                                                     >  4:45939/1‑134 (MQ=255)                                                     gcgcCGGGCCTGTTGGGCGGCAACGGCAGGCAAGTGCTCGTGGGCGCAGACTTCGCTGACCACCCGGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                           <  8:338708/135‑1 (MQ=255)                                                     gcgcCGGGCCAGGTCGGCGGCACCGGCAGGCAACTGCGCGGCGGCGCAGACTGCGCTCACCACCCCGGC‑GG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                           <  5:152598/135‑1 (MQ=255)                                                     gcgcCCGGCCAGTGCGGCGGCAACGGCAGGCACCTGCTCGTCGGCGCAGACTTCGCTCACCAACCCGGC‑GG‑CCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                           <  8:209346/135‑1 (MQ=255)                                                      cgcCCGGCCTGTTCGGCGGCAAGGGCAGGCAACTGCTGGTCGGCGCAGAGTTCGCTGACCAACCGGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                                           <  7:284132/134‑1 (MQ=255)                                                             ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCCGGGCGCGCTCGGCGCCCCCCCCCTCGTCGAGGAACAGCAGGCGCGTGGCCTGCGCGCTGCCGCTCCGcc                                                                   >  3:308363/1‑135 (MQ=255)                                                              atgTTCGGCGCCAACGGCAGGAAACTGCTCGGCGGCGCAGACTTCGCTCACCACCCCGGC‑TG‑CACGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCg                                                                  <  2:113636/133‑1 (MQ=255)                                                               aGTGCGGCGGCAACGCCAGGAAACTGCGCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                                 <  8:240063/135‑1 (MQ=255)                                                               aGTGCGGCGCCAACGGCAGCAAAGTGCTCGGGGGCGCAGACTGCGCTCACCACCCCGGC‑GG‑CCCGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                                 <  7:77143/135‑1 (MQ=255)                                                                         cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCCCCACTCAGCGAGGCACACCACGCCCGTGGCCGGCTAGCTGCCGAGCAGCCGTGGCAGGTGcc                                                       >  6:68772/1‑135 (MQ=255)                                                                                       tGCGCGGCGGCGCAGACTTCGCGGACCACCCCGCC‑GG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                         <  8:244454/135‑1 (MQ=255)                                                                                       ggCTCGCGGGCGCAGACTCCGCGCACCAACCCGCC‑GG‑CCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                         <  3:118886/134‑1 (MQ=255)                                                                                        gcggggcgGCGCAGACTCCGCTCACCACCCGGCC‑GC‑CCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                                        <  2:18529/129‑1 (MQ=255)                                                                                          tcgtcgGCGCAGACTTCGCTGACCACCCCGGC‑GG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCAt                                        <  6:146456/133‑1 (MQ=255)                                                                                            gggggCGCCGACTGCGC‑GCCCCACCCGGG‑GGCCCCGGGCGCGAGGGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                    <  2:320989/132‑1 (MQ=255)                                                                                            ggcgGCGCAGACTGCGCTGCCCCCCCGGGC‑GG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                    <  4:13187/133‑1 (MQ=255)                                                                                            ggcgGCGCAGACGTCGCCGACCAACCCGGC‑GG‑CCAGGGGGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                    <  3:271573/133‑1 (MQ=255)                                                                                             ggggCGCCGACTGCGCGCACCAACCCGGC‑GG‑CCAGGGCGCGAGCGCCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCgggg                                   <  2:245988/133‑1 (MQ=255)                                                                                               ggCGCAGCCTTCGCTGACCAACCCGGC‑GT‑CCCCGGCGCGCGCGGCGCCCCAACAATCGGGGCGGTCCAGCAGGCCCGTGGCCTGGGCGGTTGCGATACTCCGGGGCAGGTTCCCGCTGGCGCCCGCAGAGGGGCt                                 >  8:181098/1‑135 (MQ=255)                                                                                               ggCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTGGAGGAACAGCAGGCGCTTTGCCTGCTAGCTGCCGATCAGGCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                                 >  8:209114/1‑135 (MQ=255)                                                                                                 cgcAGACTTCGCGGCCCACCCGGGC‑GG‑CCTGGGGGGGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                               <  3:45939/135‑1 (MQ=255)                                                                                                 cgcAGACTGCGCTGCCCAACCCGGC‑GG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                               <  4:103316/135‑1 (MQ=255)                                                                                                  ccAGAGTTCTCAGCCCACCCCGGC‑GG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                              <  2:277973/134‑1 (MQ=255)                                                                                                      actgcccTGCCCACCCGGGC‑GG‑CCGGGGCGGGAGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                          <  7:129784/129‑1 (MQ=255)                                                                                                      aCTGCGCTCACCAACCCGGC‑GG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                          <  1:216972/135‑1 (MQ=255)                                                                                                        ttCGCTGACCACCCCGGC‑GG‑CCAGGGGGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCa                         <  8:161381/134‑1 (MQ=255)                                                                                                                ccACCCCGGC‑GG‑CCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                <  4:103898/135‑1 (MQ=255)                                                                                                                ccAACCGGGC‑GG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                <  7:235837/135‑1 (MQ=255)                                                                                                                ccAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                <  4:76427/135‑1 (MQ=255)                                                                                                                  aaCCCGGGGGG‑CCTGGGCGGTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt                <  6:14229/134‑1 (MQ=255)                                                                                                                       ggc‑gg‑cCAGGGCGCGATCGGCGCCCCCCAACTCGGCGAGGAACACCACGCGCGTTGGCTGCGCGCTGCCGCTCACCCCGTGCAGGTGCCCGCGGGCGCCGGCCTCGGGGGTGTAGCCCCTGCGGGGGTTGCCCGc         >  2:91160/1‑135 (MQ=255)                                                                                                                            g‑cCAGGGGGCGCGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTg     <  6:64745/135‑1 (MQ=255)                                                                                                                              cccgggCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                              <  5:161396/106‑1 (MQ=255)                                                                                                                              ccAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                              >  6:161396/1‑109 (MQ=255)                                                                                                                                aGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCATGGCCTGCACGCTGCCGATCAGCCGAGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCCAGCGGGTGTAGGCGGCCTTGAAg  >  8:334314/1‑135 (MQ=255)                                                                                                                                         |                                                                                                                              CGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGC‑GG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTGAAG  >  KT2440/4245020‑4245279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑