Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,830,444 C→T 7.3% P235L (CCC→CTC)  CJ019_05460 → putative zinc protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,830,4440CT 7.3% 172.1 / 3.5 55P235L (CCC→CTC) CJ019_05460putative zinc protease
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

CTGGTACCACCCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTACGCGCTGCGTCTGCTTCCCG  >  KT2440/5830310‑5830578
                                                                                                                                      |                                                                                                                                       
cTGGTACCACCCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGcc                                                                                                                                         <  1:813/135‑1 (MQ=255)
cTGGTACCACCCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGAAGCGGCCCTTACCCACAGGGcc                                                                                                                                         >  4:331901/1‑135 (MQ=255)
 tGGTACCACCCCAACAATGCCACCCTCGCCGTGGCCGGCGACCTCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGCTCCCCGCCCCTCGCTTGCCGATGCGGCCCTTACCCCCAGGGccc                                                                                                                                        >  1:232677/1‑135 (MQ=255)
     accaccCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGCTCCCCGCCCATCGCTTGCAGATGCGGCCCTTACCCACCGGGCcctcct                                                                                                                                    >  3:270040/1‑135 (MQ=255)
     accaccCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACCGGGCcctcct                                                                                                                                    >  6:234688/1‑135 (MQ=255)
     accaccCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCcctcct                                                                                                                                    >  4:307499/1‑135 (MQ=255)
     accaccCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCcctcct                                                                                                                                    >  8:247511/1‑135 (MQ=255)
     accaccCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAAATACAAACCTTGGTGACGCGCCACATTGCGGCGCTCCCCGCCCATCGCATGCCCATGCGGCCCTTACCCCCACGGCcctcct                                                                                                                                    >  5:336632/1‑135 (MQ=255)
     accaccCCAACAAACCCACCCTCGCCGTGGCCGGCGACATCAGCCAGCAACAACTACAAACCTTGATGACGCGCCACAATTCGGCGATCCCCGCCCATCGCTTGCCGATGCGGACCTTACCCACACGGCccgcct                                                                                                                                    >  7:317706/1‑135 (MQ=255)
            caacaaTGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAACCCTTGGTGACCCGCCCCATTGCGGCGCTCCCCCCCCATCCCTTGCCCATGCGGCCCGTACCCACACGGCCCGCCTCCCAggg                                                                                                                             >  7:286748/1‑135 (MQ=255)
            caacaaTGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGCTCCCCGCCCATCGCTTGCCGCTGCGGCCCTTCCCCCCAGGGCCCTCCTCCCAggg                                                                                                                             >  2:205763/1‑135 (MQ=255)
            caacaaTGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAggg                                                                                                                             >  5:141525/1‑135 (MQ=255)
            caacaaTGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAggg                                                                                                                             >  5:2726/1‑135 (MQ=255)
            caacaaTGCCACCCTCGCCCTGGCCGGCGACATCACCCTGCTACAACTACAAACCTTGGTCCCGCGCCACTTTGCCGCCATCCCCGCCCATCGCTTGCCGATGCGGCCCTAACCCACAGGACCCTCATCCCAcgg                                                                                                                             >  8:169640/1‑135 (MQ=255)
             aacaaTGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGc                                                                                                                            >  2:71912/1‑135 (MQ=255)
                     cACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGCTGCGGCCCGTACCCCCAGGGCCCGCCTCCCAGGGCCGGCgggt                                                                                                                    >  8:328290/1‑132 (MQ=255)
                     cACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGttt                                                                                                                    >  6:293786/1‑135 (MQ=255)
                      aCCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGttt                                                                                                                    >  2:156642/1‑134 (MQ=255)
                          tCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCCCTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCGCCTCCCAGGGCCGGCGTTTTCAGa                                                                                                               >  6:171177/1‑135 (MQ=255)
                          tCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGa                                                                                                               >  1:183942/1‑135 (MQ=255)
                              cGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTTCCGATGCGGCGCTTACCCACAGGTCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCt                                                                                                           <  7:10111/135‑1 (MQ=255)
                               gTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTg                                                                                                          >  8:216649/1‑135 (MQ=255)
                                   ccGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGcc                                                                                                      <  6:171302/135‑1 (MQ=255)
                                   ccGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGcc                                                                                                      <  2:312951/135‑1 (MQ=255)
                                   ccGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGcc                                                                                                      <  2:220296/135‑1 (MQ=255)
                                         aCATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAg                                                                                               >  3:240274/1‑135 (MQ=255)
                                               gCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGAGCCTG‑CCAGGCTTGc                                                                                         >  8:85311/1‑135 (MQ=255)
                                                     tacaactacaaACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACg                                                                                   <  1:225895/135‑1 (MQ=255)
                                                     tacaactacaaACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACg                                                                                   <  5:92336/135‑1 (MQ=255)
                                                     tacaactacaaACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACg                                                                                   <  8:162/135‑1 (MQ=255)
                                                     tacaactacaaACCTTCGTGACGCGCCACTTTGAGGCGATCCCCGCCCATCGCTTGCCGTTGCGGCCCTTACCCACAGGGCCCTCCTCCCAGCTCCGGCGATTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACg                                                                                   <  8:11313/135‑1 (MQ=255)
                                                             caaACCTTGGTGACGCGCCACTTTGCGGCGATCACCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATc                                                                           >  5:49330/1‑135 (MQ=255)
                                                              aaaCCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGAt                                                                            <  1:156642/133‑1 (MQ=255)
                                                               aaCCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCAt                                                                         <  6:259628/135‑1 (MQ=255)
                                                                 ccTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATaa                                                                       >  3:147002/1‑135 (MQ=255)
                                                                      gTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCt                                                                    <  7:194268/133‑1 (MQ=255)
                                                                       tGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCt                                                                                                           <  2:322643/94‑1 (MQ=255)
                                                                       tGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCt                                                                                                           >  1:322643/1‑94 (MQ=255)
                                                                            cgcCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTa                                                            <  1:71912/135‑1 (MQ=255)
                                                                            cgcCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTa                                                            <  1:28776/135‑1 (MQ=255)
                                                                              ccACTTTGCGGCGATGCCCGGTCATCGCTTGCCGATGGGGCCCTTACCCACAGGGCCCTCCTAGCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCTCAACGGCGTGATCATAAGCTTCAAACTa                                                            <  8:305606/133‑1 (MQ=255)
                                                                               cACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTAc                                                           >  7:220429/1‑133 (MQ=255)
                                                                               cACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGGGATCATAAGCTTGAAACTAccc                                                         >  3:2938/1‑135 (MQ=255)
                                                                               cACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGGGATCATAAGCTTCAAACTAccc                                                         >  8:137238/1‑135 (MQ=255)
                                                                                     gcggcgATCCCCGCCCATCGCTTTCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACccagcc                                                     <  1:71288/133‑1 (MQ=255)
                                                                                            tCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACcg                                            <  7:212280/135‑1 (MQ=255)
                                                                                              cccGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGGGCTCATAAGCTTCAAACTACCCAGCCAGCGTACcgcg                                          >  3:66108/1‑135 (MQ=255)
                                                                                               ccGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTGTTGCGCCGCGGCGCCGGCCCGGGGTTGC‑CAACGGCGGGGTGCTAAGCTTCAAACAACCCCCCCCGCGTTCCccgc                                         >  2:335646/1‑135 (MQ=255)
                                                                                                       cGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCAGAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 <  7:26738/135‑1 (MQ=255)
                                                                                                       cGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 <  6:336632/135‑1 (MQ=255)
                                                                                                       cGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 <  4:114378/135‑1 (MQ=255)
                                                                                                       cGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 <  5:208537/135‑1 (MQ=255)
                                                                                                         cTTGCCGATGCGGCGCTTACGCACAGGGCCCTCCTGCCACGGCCGGCGGTTTCAGACGCGGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCcag                               <  6:271319/135‑1 (MQ=255)
                                                                                                         cTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCcag                               <  2:260201/135‑1 (MQ=255)
                                                                                                         cTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCGTCAACCTACCCAGCCAGCGTACCGCGCAGTCTGCcag                               >  3:327321/1‑135 (MQ=255)
                                                                                                               gATGCGGCCCTTACCCACAGGGCTCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 >  4:127046/1‑127 (MQ=255)
                                                                                                               gATGCGGCCCTTACCCACAGGGCTCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 >  4:132375/1‑127 (MQ=255)
                                                                                                               gATGCGGCCCTTACCCACAGGGCTCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 <  3:132375/127‑1 (MQ=255)
                                                                                                               gATGCGGCCCTTACCCACAGGGCTCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGcc                                 <  3:127046/127‑1 (MQ=255)
                                                                                                                  gCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGAGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTa                      <  6:296624/135‑1 (MQ=255)
                                                                                                                                   ggCCCTCCTCCCAGGGCCGGCGTTTTCAGCCGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTACGCGCTGCGTCTGCTTc     >  3:266029/1‑135 (MQ=255)
                                                                                                                                   ggCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTa                                                            <  3:247483/80‑1 (MQ=255)
                                                                                                                                   ggCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTa                                                            >  4:247483/1‑80 (MQ=255)
                                                                                                                                   ggCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTACGCGCTGCGTCTGCTTc     >  4:87147/1‑135 (MQ=255)
                                                                                                                                     cccTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTa                                                            <  5:332178/78‑1 (MQ=255)
                                                                                                                                     cccTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTa                                                            >  6:332178/1‑78 (MQ=255)
                                                                                                                                      cctcctCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTACGCGCTGCGTCTGCTTCCCg  >  4:274118/1‑135 (MQ=255)
                                                                                                                                      cctcatCACAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTACGCGCTGCGTCTGCtt      >  8:47971/1‑131 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CTGGTACCACCCCAACAATGCCACCCTCGCCGTGGCCGGCGACATCAGCCTGCTACAACTACAAACCTTGGTGACGCGCCACTTTGCGGCGATCCCCGCCCATCGCTTGCCGATGCGGCCCTTACCCACAGGGCCCTCCTCCCAGGGCCGGCGTTTTCAGACGCTGCGCCTG‑CCAGGCTTGCACAACGGCGTGATCATAAGCTTCAAACTACCCAGCCAGCGTACCGCGCAGTCTGCCAGGCAGGCCTACGCGCTGCGTCTGCTTCCCG  >  KT2440/5830310‑5830578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑