Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440126,2790GA26.8% 96.8 / 34.6 56A581T (GCG→ACG) CJ019_00134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (15/26);  new base A (3/12);  total (18/38)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATCTGGCTGGGGACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGACCAGGTGCTCAA  >  KT2440/126145‑126412
                                                                                                                                      |                                                                                                                                     
aTCTGGCTGGGGACAAGGTCCTCAAGATCGTGGCCGACTAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATAGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTg                                                                                                                                       >  1:31559/1‑135 (MQ=255)
 tCTGGCTGGGGACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTgc                                                                                                                                      <  1:199664/135‑1 (MQ=255)
     gctggGGACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCaa                                                                                                                                  >  7:282055/1‑135 (MQ=255)
        ggggACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTg                                                                                                                               >  2:16678/1‑135 (MQ=255)
        ggggACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGGGATTTCATCGCGCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTg                                                                                                                               <  5:250551/135‑1 (MQ=255)
          ggACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTg                                                                                                                               <  8:295092/133‑1 (MQ=255)
              aaGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAg                                                                                                                         <  8:4968/135‑1 (MQ=255)
                ggTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGgt                                                                                                                       <  6:69514/135‑1 (MQ=255)
                   ccTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGtt                                                                                                                    <  3:220030/135‑1 (MQ=255)
                   ccTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGGTTTGGCGGCGCGGAATTCGTCATGCTGCTGCCGCCGACTACGCCCCCAGTCGCTGCGCAAGGGGCCGAGGTGtt                                                                                                                    >  3:34224/1‑135 (MQ=255)
                        aGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGCTTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCCGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               >  1:106605/1‑135 (MQ=255)
                        aGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               <  1:187326/135‑1 (MQ=255)
                        aGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               <  8:51606/135‑1 (MQ=255)
                        aGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               <  6:203031/135‑1 (MQ=255)
                        aGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               <  8:163896/135‑1 (MQ=255)
                        aGATCGTGGCCGACCAGTTGCGCAAGCGGCTGCGGGGCCGTGAGTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               <  7:174322/135‑1 (MQ=255)
                            cGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCcac                                                                                                           <  5:297083/135‑1 (MQ=255)
                            cGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCcac                                                                                                           <  3:279871/135‑1 (MQ=255)
                               ggCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGGCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTAAGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTGGCGTGCCACAGt                                                                                                        >  7:319156/1‑135 (MQ=255)
                                 ccGACCAGTTGCGCAAGCGTCTGTGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCg                                                                                                      >  6:26913/1‑135 (MQ=255)
                                 ccGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAggc                                                                                                       >  6:44212/1‑132 (MQ=255)
                                  cGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGa                                                                                                     <  3:21311/135‑1 (MQ=255)
                                            gactggCGTCGGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCa                                                                                           <  5:191489/130‑1 (MQ=255)
                                            cgcAAGCGTCTGCGGGGCCGTGATTTAATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCa                                                                                           <  2:2877/135‑1 (MQ=255)
                                             gcAAGCGTCTGTGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCa                                                                                           <  5:26913/134‑1 (MQ=255)
                                             gcAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCAAAGCCTGCCCa                                                                                           <  1:16678/134‑1 (MQ=255)
                                                         ggggCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGCCTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAgg                                                                              >  5:159784/1‑135 (MQ=255)
                                                         ggggCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGGAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCa                                                                                 <  1:229820/132‑1 (MQ=255)
                                                               gTGATTGCAGCGCCCGTTTTGGCGGCGAGGAATTCGTCCTGCTGCTGCCGCAGACTACGCCCGCAGGCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCCCTTCACgggcgcac                                                                        <  4:129563/135‑6 (MQ=255)
                                                                   tttCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACGTCAAGGGAGCACGggt                                                                    >  6:319206/1‑135 (MQ=255)
                                                                         cGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGAt                                                              <  3:289694/135‑1 (MQ=255)
                                                                          gCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATc                                                             >  8:275437/1‑135 (MQ=255)
                                                                          gCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCACTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATc                                                             >  1:310172/1‑135 (MQ=255)
                                                                           cccGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATc                                                             <  4:193261/134‑1 (MQ=255)
                                                                            ccGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATcac                                                           <  7:238661/135‑1 (MQ=255)
                                                                            ccGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATcac                                                           <  8:308012/135‑1 (MQ=255)
                                                                                  tGGCGTCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCt                                                      <  8:101885/134‑1 (MQ=255)
                                                                                  tGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCt                                                                                                <  1:326699/92‑1 (MQ=255)
                                                                                  tGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCt                                                                                                >  2:326699/1‑92 (MQ=255)
                                                                                       tcgAGGAATTGTGCATGCTGCTGCCGATGAATACGCCGGCTGGCGCGGCGAATGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCTTTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCg                                                <  7:126977/134‑1 (MQ=255)
                                                                                       gcgAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCg                                                <  1:56153/135‑1 (MQ=255)
                                                                                          aGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGcc                                             <  6:136904/135‑1 (MQ=255)
                                                                                           ggAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATca                                                            <  1:124510/119‑1 (MQ=255)
                                                                                           ggAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATca                                                            >  2:124510/1‑119 (MQ=255)
                                                                                            gAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTg                                           <  3:23176/135‑1 (MQ=255)
                                                                                             aaTTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTgg                                          >  7:2136/1‑135 (MQ=255)
                                                                                                  gTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGc                                      <  4:82046/134‑1 (MQ=255)
                                                                                                  gTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGc                                      <  4:280603/134‑1 (MQ=255)
                                                                                                  gTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGc                                      <  8:2136/134‑1 (MQ=255)
                                                                                                          gctgcCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               >  4:64197/1‑53 (MQ=255)
                                                                                                          gctgcCGCAGACTACGCCCGCAGTCGCTACGCAAGTGGCCGAGGTGTTGCGTg                                                                                                               <  3:64197/53‑1 (MQ=255)
                                                                                                                          cccGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGGCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGAc             <  1:242195/135‑1 (MQ=255)
                                                                                                                          cccGCAGGCGCTGCGCAAGTGGCCGAGGTGTTGCGGGCCACAGTCGAACCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCAGCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGAc             <  4:34224/135‑1 (MQ=255)
                                                                                                                           ccGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGggt                                                                    >  7:258641/1‑79 (MQ=255)
                                                                                                                           ccGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGggt                                                                    <  8:258641/79‑1 (MQ=255)
                                                                                                                           ccGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGa              >  2:297360/1‑133 (MQ=255)
                                                                                                                                 tCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGACCAGGTGc      >  3:325518/1‑135 (MQ=255)
                                                                                                                                     tGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGACCAGGTGCTCaa  <  3:238873/135‑1 (MQ=255)
                                                                                                                                      gcgcAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGACCAGGTGCTCa   >  3:111350/1‑133 (MQ=255)
                                                                                                                                      |                                                                                                                                     
ATCTGGCTGGGGACAAGGTCCTCAAGATCGTGGCCGACCAGTTGCGCAAGCGTCTGCGGGGCCGTGATTTCATCGCCCGTTTTGGCGGCGAGGAATTCGTCATGCTGCTGCCGCAGACTACGCCCGCAGTCGCTGCGCAAGTGGCCGAGGTGTTGCGTGCCACAGTCGAAGCCTGCCCATTCCACTTCAAGGGAGAACGGGTGGTGATCACCACCTCCATCGGCCTGGGTGCATTCCGTTCTGGCGAACGCAGCGACCAGGTGCTCAA  >  KT2440/126145‑126412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑