Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440932,0260CG28.1% 119.1 / 11.9 57A2216P (GCA→CCA) CJ019_00881hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (3/38);  new base G (16/0);  total (19/38)
Fisher's exact test for biased strand distribution p-value = 1.67e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCGGTG  >  KT2440/931901‑932159
                                                                                                                             |                                                                                                                                     
acTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCTGGCTGGGGGCGTGGGTGGCGGGAgtta                                                                                                                              >  3:160922/1‑134 (MQ=255)
  tGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGGTGGCGGGGGGGGGGGGTGGCGGGGtttttt                                                                                                                            >  6:212553/1‑132 (MQ=255)
  tGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCGGGCGGGGGTCGTGGGTGGCGGGATTTCat                                                                                                                            >  4:236507/1‑135 (MQ=255)
   gCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGGTGGCTGGGGTCGTGGGTGCCGGGGtttgaaa                                                                                                                           >  5:163919/1‑131 (MQ=255)
     cGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCata                                                                                                                           <  2:257683/133‑1 (MQ=255)
     cGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGGTGGCGGGAGTTGAtttt                                                                                                                         >  1:16475/1‑135 (MQ=255)
      gACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTg                                                                                                                        <  6:54853/135‑1 (MQ=255)
       aCCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGGTGGCGGGGGTGGTGGGTGGCGGGGTTTGataattg                                                                                                                       >  6:306014/1‑131 (MQ=38)
       aCCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCGGGCTGGGGTCGTGGTGGGCGGGCTTTCATAtt                                                                                                                         >  8:318784/1‑133 (MQ=255)
        ccAGTGTGCCGTTGGCATCGTGGACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCa                                                                                                                      <  3:134746/135‑1 (MQ=255)
          aGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCGGCCACCACCAGGTCGCGTGGGCTGGCGGGGGTCGTGGGTGGCGGGGTTTGATATTGGAgg                                                                                                                    >  4:115850/1‑135 (MQ=38)
                 cGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCTGGCTGGGGTCGTGGGTGCCGGGATTTGAGATTGCAGGGcctg                                                                                                               >  6:315047/1‑130 (MQ=255)
                    tGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGGCGGGATTTCATATTGCAGGGCTTCAGAgg                                                                                                          >  4:204153/1‑135 (MQ=255)
                       cATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCAGTTCGCAGCCACCACCAGGACGCTTGGGCTGGCGGGGCTCCTGGTTGGCCGGATTTTATATTTCCGGGAATAAAAGGCGt                                                                                                       >  4:228742/1‑135 (MQ=255)
                        aTCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGtt                                                                                                      <  5:291063/135‑1 (MQ=255)
                         tCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGGCGGGATTTCATATTTCAGGGCTTCAGAGGCGTTg                                                                                                     >  3:149353/1‑135 (MQ=255)
                           gtgtACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGGTGGCGGGGGTTGAAATTTCACGGGTTAAGAGGCGTTggc                                                                                                   >  5:158822/1‑133 (MQ=255)
                            tgtACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGcc                                                                                                   <  5:80968/134‑1 (MQ=255)
                               aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTTGGCTGGCTGGGGTCGTGGTTGCCGGGATTTGATTTTGGAGGGCTTCAGAGGCGTTTCCGCTc                                                                                               >  7:280692/1‑135 (MQ=255)
                               aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                               <  6:170963/135‑1 (MQ=255)
                               aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                               <  1:203535/135‑1 (MQ=255)
                               aCTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTc                                                                                               <  1:279419/135‑1 (MQ=255)
                                 ttCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCg                                                                                              <  4:328119/134‑1 (MQ=255)
                                 ttCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCg                                                                                              <  7:279748/134‑1 (MQ=255)
                                 ttCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCg                                                                                              <  5:315047/134‑1 (MQ=255)
                                 ttCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCg                                                                                              <  3:236507/134‑1 (MQ=255)
                                  tCCACCCGGCGGCCCGTCGGGGCTGTACGAGTGAGGCTGGCGCCGTTCGCAGCCACGACCAGGTCGCTGGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCa                                                                                            <  2:3006/135‑1 (MQ=255)
                                     acacGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATc                                                                                          <  4:17045/134‑1 (MQ=255)
                                      cacGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGGTGGCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCGCGCCTCg                                                                                         >  8:90326/1‑134 (MQ=255)
                                              cccGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCGGGCTGGGGTCGTGGGTGGCGGGAGTTGATATTTCACGGCTTCAGCGGCGTTTCCGCGCGCCTCGGGCTGcccc                                                                                >  8:89878/1‑135 (MQ=255)
                                                 gacagggCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGGCTGCACCAgg                                                                             <  3:41234/131‑1 (MQ=255)
                                                 gTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCGTGGGCTGGCTGGGGTCGGGGTTGGCGGGCTTTGATATTTCAGGGGGTCAGCGGCGTTGCCGCTCGCATCGGTCTGCCCCCgg                                                                             >  4:45258/1‑135 (MQ=255)
                                                                  gATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCgg                                                            <  1:25826/135‑1 (MQ=255)
                                                                    tGCTGGCGCCGTTCGCAGCCACCTCCAGGTCGGTTGGGCTGGCTGGGGTCGTGGGTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGtt                                                          <  8:125491/135‑1 (MQ=255)
                                                                        ggCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGGCGGGGTTTGATATTGGAGGGCGTAAGAGGCGGTGCCGCTGGCATCGGTCTGCCCCAGGCGCAATTTCTCGCCCGGGTgggc                                                      >  7:272021/1‑135 (MQ=255)
                                                                         gcgcCGTTCGCAGCCACCACCAGGTCGCGTGGGGTGGCTGGGGGCGTGGGTGGCGGGGTTTCATAATGCACGGCGTGAGATGCGTTGCCCCGCGCCTAGGTCTGCACCAGGCGCCAATGCGCGCCCGGGTgcggg                                                     >  8:186417/1‑133 (MQ=255)
                                                                                   cAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGa                                           <  7:318784/135‑1 (MQ=255)
                                                                                   cAGCCACCACCAGGTCGCTGGGGCTGGCGGGGGGCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGa                                           <  8:125784/135‑1 (MQ=255)
                                                                                   cAGCCACCACCAGGGCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGa                                           <  8:140741/135‑1 (MQ=255)
                                                                                     gCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAgg                                         <  8:201761/135‑1 (MQ=255)
                                                                                                                    tCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTGGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtg          <  1:320024/135‑1 (MQ=255)
                                                                                                                      gtggtgGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  5:306014/135‑1 (MQ=255)
                                                                                                                      gTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  5:212553/135‑1 (MQ=255)
                                                                                                                      gTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCGCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  4:232036/135‑1 (MQ=255)
                                                                                                                      gTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGGTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  7:175212/135‑1 (MQ=255)
                                                                                                                      gTGGTTGCCGGGATTTCATATTGCAGGGCGTCAGAGGCGTGGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  8:272021/135‑1 (MQ=255)
                                                                                                                      gTGGGTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGGGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  5:76407/135‑1 (MQ=255)
                                                                                                                      gTGGGTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCGCGCCCGGTTGCGCGGCTGGGGTGAGGGCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  1:285507/135‑1 (MQ=255)
                                                                                                                       tGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCTTTGCCGCTCGCATCGGTCGGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  3:325420/134‑1 (MQ=255)
                                                                                                                       tGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  6:163919/134‑1 (MQ=255)
                                                                                                                       tGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  1:82446/134‑1 (MQ=255)
                                                                                                                       tGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  1:198470/134‑1 (MQ=255)
                                                                                                                       tGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGGCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACtgtg        <  4:140649/134‑1 (MQ=255)
                                                                                                                        ggTTGCCGGGATTTCTTATTGCAGGGCTTCAGTGGCGGTGCCGCTCGCATCGGTCGTCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGATGGTTCCGTTGGCGCCGACTGTGGc      <  6:256185/135‑1 (MQ=255)
                                                                                                                        ggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGc      <  7:90326/135‑1 (MQ=255)
                                                                                                                        ggTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGc      <  3:204153/135‑1 (MQ=255)
                                                                                                                            gCCGGGATTTCATCTTGCAGGGCTTCAGAGGCGGGGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGGTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCGGTg  <  8:259614/135‑1 (MQ=255)
                                                                                                                             ccGGGATTTCATATTGCAGGGCTGCAGAGGCGGTGCCGCTCGCATCGGTCTGCACCAGGCGCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCGGTg  <  3:310005/134‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
ACTGCCGACCAGTGTGCCGTTGGCATCGTGTACTTCCACACGGCTGCCCGTCGGGGCTGTACCAGTGATGCTGGCGCCGTTCGCAGCCACCACCAGGTCGCTTGGGCTGGCTGGGGTCGTGGTTGCCGGGATTTCATATTGCAGGGCTTCAGAGGCGTTGCCGCTCGCATCGGTCTGCACCAGGCTCAATTGCTCGCCCGGTTGCGCGGCTGGGGTGAGGTCGACCAAGAAGGTTCCGTTGGCGCCGACTGTGGCGGTG  >  KT2440/931901‑932159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑