Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,393,9360TG28.6% 114.9 / 14.7 63intergenic (+46/‑123)cpoB/queECell division coordinator CpoB/7‑carboxy‑7‑deazaguanine synthase
Reads supporting (aligned to +/- strand):  ref base T (12/33);  new base G (18/0);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 3.34e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCA  >  KT2440/1393807‑1394063
                                                                                                                                 |                                                                                                                                 
aaaGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGtttttt                                                                                                                              >  5:142859/1‑135 (MQ=255)
 aaGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTc                                                                                                                             >  4:328732/1‑135 (MQ=255)
          ttCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTA‑ggaa                                                                                                                    >  6:298687/1‑131 (MQ=255)
           tCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCa                                                                                                                  >  1:38697/1‑135 (MQ=255)
           tCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTTGTTT‑GAAta                                                                                                                   >  7:2084/1‑133 (MQ=255)
                     gTGCCCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTT‑GAAAGACTGcccggtt                                                                                                        >  7:226288/1‑131 (MQ=255)
                     gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTT‑GATTGACTGgccggtt                                                                                                        >  1:108791/1‑131 (MQ=255)
                     gTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGGTT‑GAATCACTTcccggtt                                                                                                        >  2:74674/1‑131 (MQ=255)
                       gACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGGACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTGTTGTTGTTT‑GGTTCACGGGCCtgtttt                                                                                                      >  3:29443/1‑135 (MQ=255)
                            aGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCTCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTaa                                                                                                 <  8:156472/135‑1 (MQ=255)
                            aGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTaa                                                                                                 <  6:139030/135‑1 (MQ=255)
                                ccccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTGGTTA‑GCATCACTGGCCTTTTTTAGTTAacac                                                                                             >  8:5399/1‑135 (MQ=255)
                                 cccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTCTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTGTTTCGTTT‑TGAAGACTGCCCCTTTTTAGTataaacc                                                                                            >  4:143977/1‑129 (MQ=255)
                                   cGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACgct                                                                                          <  6:71511/135‑1 (MQ=255)
                                   cGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACgct                                                                                          <  7:173198/135‑1 (MQ=255)
                                   cGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACgct                                                                                          <  4:258893/135‑1 (MQ=255)
                                        ccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                     <  5:311867/135‑1 (MQ=255)
                                        ccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                     <  5:23805/135‑1 (MQ=255)
                                        ccTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCtgctgc                                                                                     <  4:274448/135‑1 (MQ=255)
                                          tctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCgg                                                                                   <  6:142859/135‑1 (MQ=255)
                                           ctGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTGTTTTGGTTA‑GAATACATGCCCTTTTTTGGTAAACACGCGGCTGCggg                                                                                  >  4:65416/1‑134 (MQ=255)
                                              ccGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTA‑GAATCACTGCCCTTGTTTCGGAAACACGCGGCTGCGGATaa                                                                               >  4:216782/1‑135 (MQ=255)
                                                   cAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTT‑TGATCACTGCCCTTTTTTGGTTAACACGCGGCGGCGGATAACGcga                                                                          >  1:221211/1‑133 (MQ=255)
                                                     aCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTAt                                                                         <  2:38697/134‑1 (MQ=255)
                                                      cTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGt                                                                       <  6:56407/135‑1 (MQ=255)
                                                           ccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTGTTTTAGGTA‑GAATCACTGCCCATTTTTCGGAACCACGCTGCTGCGGTGAACGCTATGTagagg                                                                  >  4:114342/1‑134 (MQ=255)
                                                              gCGTGACCTGCAGACGCTCTAGGCGTTGGCGCTGTACCGCACTAAATAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCcg                                                              <  5:298687/135‑1 (MQ=255)
                                                              gCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCcg                                                              >  8:68157/1‑135 (MQ=255)
                                                              gCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCcg                                                              <  7:68157/135‑1 (MQ=255)
                                                                gTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCcgcg                                                            <  2:39419/135‑1 (MQ=255)
                                                                gTGACCTGCAGAAGCTCTAGGCGTGGTCGCTGTACCGCTCGAAAGAAACCCGCGCCACGCGCGGGTTTATTCGGAA‑CAATGACTGCCATTTTTTGGTAAACACGCTGATGGGGTTAAAGCAATGTCGAG‑TCcgcg                                                            <  2:285904/135‑1 (MQ=255)
                                                                           aaGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTGGGTT‑GGATGACTGGCCGTTTTTGGTAAACACGCGGCTGCGGGTAACGCTATGTCGAG‑TCCGCGCCAGTGCCTgg                                                 >  3:35066/1‑134 (MQ=255)
                                                                                 tAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTT‑GAATCCCGGCCCTTTTTTCGGAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGCCCGTGCCTGACGGAgg                                           >  4:261653/1‑135 (MQ=255)
                                                                                   ggCGTTTGCGCTGTACCGCTCGAAAGAACCCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGc                                          <  8:298242/134‑1 (MQ=255)
                                                                                    gCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCgg                                        <  3:136030/135‑1 (MQ=255)
                                                                                    gCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCg                                         <  3:324224/134‑1 (MQ=255)
                                                                                     cGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTTAACACGCGGCTGCGGATAACGCTATGTCGAG‑TCCGCGACCGTGCCTGCcggcggcggc                                       >  8:44778/1‑134 (MQ=255)
                                                                                      gTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGTTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGAc                                      <  8:96897/135‑1 (MQ=255)
                                                                                      gTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGAc                                      <  4:126850/135‑1 (MQ=255)
                                                                                      gTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGAc                                      <  6:291085/135‑1 (MQ=255)
                                                                                      cagggcGCTGGACCGCTCGAAAGAAACCCGCGCCAGGCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGAc                                      <  8:30178/130‑1 (MQ=255)
                                                                                      acagTCGCGGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGAc                                      <  5:218435/132‑1 (MQ=255)
                                                                                           cGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCt                                 >  4:170062/1‑135 (MQ=255)
                                                                                           cGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTT‑GAATCACAGGCCGATTTTTGTTAACACGCTGCGGGGGTTTACGCTAATTAGAA‑TCCGCCAACGTGCCGGACGGCGGCGGACAGcca                                 >  2:62499/1‑134 (MQ=255)
                                                                                            gCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTGGTTA‑TAATCACTGCCCGTTTTTAGTAAAAACGCTGCTGCGGATAACGCTATTTCGAG‑TCCGCGCCAGTGCCGGACGGAGGCGGCCAGccgg                                >  4:324224/1‑133 (MQ=255)
                                                                                                   cGCTCGAATGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGGTGGTGCGGATAACGCTTTGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGc                          <  6:259096/134‑1 (MQ=255)
                                                                                                    gCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTg                        <  8:277704/135‑1 (MQ=255)
                                                                                                    gCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTg                        <  8:2084/135‑1 (MQ=255)
                                                                                                       cGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTT‑GAATCACTGCCCGTTTTTCGTAAACACGCGGCTGCGGGTAACGCTATGTCGAG‑TCCGCGACAGTGCCGGTCGGGGGCGGGCAGGCTGTTTAGCTGTCa                     >  4:293693/1‑135 (MQ=255)
                                                                                                       cGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGCTAACGGGATGTCGCG‑TCCGCGACCGTGCCTGCCGGCGGCGGTCAGCCGGTTTAGCTGTcc                     >  2:84470/1‑134 (MQ=255)
                                                                                                       cGAAAGAAACCCGCGCCAGTCGCGGGGTTTTTCGGTA‑GCATCACGGCCCGTTTTTCGTAAACCCGCGGCTGCGGCTAACGCGATGTCGCG‑TCCGCGACAGTGCCGGACGGAGGCGGCCCGCCGGTTTAGcggctg                     >  7:30178/1‑130 (MQ=255)
                                                                                                       cGAAAGAAACCCGCGCCAGTCGCGGGGGTTTTGGTTT‑GTATACCGGCCCTTTTTTGGTTAACACGCTGCTGGGGTTATCGCGATTTCGAG‑TGCGCGACAGTGGCTGACGGCGGCGGCCCGCCTGGTTTGCTggga                     >  2:320644/1‑132 (MQ=255)
                                                                                                        gaaagaaaCCCGCGCCAGTCGCGGGTTTTTTGGTTA‑GAATACCTGCCCGTTTTTGGTAAAAACGCTGCGGGGGCGAAAGCTATGTCGAG‑TCCGCGGCAGTTCCGGACCGCGGCGGGCAGCCGGTTTAGCGGTCAc                    >  4:140112/1‑135 (MQ=255)
                                                                                                        gaaagaaaCCCGCGCCAGTCGCGGGTTTTTTCGTTT‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGCCAGTGCCTGCCGGAGGCGGCCAGCCGGTTTAGCTGTCAc                    >  7:101906/1‑135 (MQ=255)
                                                                                                        gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTCGGTT‑GAATGACTGCCCGTTTTTGGGAAACACGCGGCGGCGGATTACGGTATGTCGAG‑TCCGCGCCAGTGCCTGCCGGAGGCGGGCAGCCTGTTTTGCTGTCAc                    >  6:135694/1‑135 (MQ=255)
                                                                                                        gaaagaaaCCCGCGCCAGTCGCGGGGTTTTTCGGTA‑GAATCACAGGCCGTTTTTGGGAAACACGCTGCGGCGGATATCGCTATGTTGCG‑TCCGGGGCAGTGCCTGACGGAGGCGGTCAGCCTGGTTAGCTGTCAc                    >  2:16359/1‑135 (MQ=255)
                                                                                                         aaagaaaCCCGCGCCAGTCGCGGGGTTTTTGGTTA‑GAATCACTGCCCGTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGCCAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTGACg                   >  8:13776/1‑135 (MQ=255)
                                                                                                            gaacCCCGCGCCCGTCGGGGGTTTTTTCGTAA‑GAATCACTGCCCTTTTTGCGTAAACCCGCGGCTGCGGATAACGCTATGTCGAG‑GCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGccc                <  6:264348/135‑1 (MQ=255)
                                                                                                                cccGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTgg            <  7:210432/135‑1 (MQ=255)
                                                                                                                  cggcccAGTCG‑GGGTTTTTTAGGTATGAAGACCTCCCCTTTTTCCGTAACCCCGCT‑CTGCCGTTAACGCTAAGTCCCGTTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCg          <  8:271349/131‑1 (MQ=255)
                                                                                                                  cgcgCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCg          <  2:108791/135‑1 (MQ=255)
                                                                                                                      ccAGGCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCa      <  3:140112/135‑1 (MQ=255)
                                                                                                                       cAGTCGGGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCAt     <  1:62499/135‑1 (MQ=255)
                                                                                                                          gggcgGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACCCGCTGCTGCGGATAACGCTATGGCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCa  <  4:227370/133‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                 
AAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTTTCGTTA‑GAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCTATGTCGAG‑TCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCA  >  KT2440/1393807‑1394063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑