Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,524,7320TG62.5% 0.1 / 17.9 24Q67P (CAG→CCG) CJ019_01439hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/3);  new base G (0/15);  total (6/18)
Fisher's exact test for biased strand distribution p-value = 6.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCT  >  KT2440/1524598‑1524838
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aCGCCACATGGCTGTGCTCGGGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGGGCGCCAGGCGCTCGGCCTGGTTCAGCAGCTGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCt                                                                                                            <  4:102249/135‑1 (MQ=255)
      caTGGCCGTGCGCGGGGGCTGCGGGTGGCAACTGGCTTTCCAGCTGGCGCTGCATGTGCTCCAGGCGCGGGGCCTGTTTCAGCAGCAGGCGCTGCGGGCGCACCTGGCGCAGCAGGGGGTAAGGTGGGTgggggg                                                                                                      <  4:18349/135‑1 (MQ=255)
                 tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGttt                                                                                            >  3:306514/1‑134 (MQ=255)
                   gcgGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTa                                                                                         >  5:52120/1‑135 (MQ=255)
                           gctgTTGAAACTGGCTTTCTAGATGGCGTTGAATGTGCGCCAGGCGCGCGGCGGGGTTCAGCAGCAGGCGCCGCGGGCGCACCTGGCGAAGCTGGGGGTAAGGGGGCTGGGGGGGGGTGGGTTTGTAGGCCATgg                                                                                 <  4:287871/132‑1 (MQ=255)
                                       ggCTTTCCAGCCGGCGCTGCAAGGGCGCCCGGCGCGGGGCCTGGTGCAGCAGCGGGGGCCGCGGGCGCCCCGGGCGCCGCAGGGCGGAAGGGGGCGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                     <  2:53212/135‑1 (MQ=255)
                                         cTTTCCCGCCGGCGGGGCAAGTGGTCCCGGGGCGGGGCGTGGTGCAGCAGCAGGCGCGGCGGGGGCACGTGGCGCAGCCGGGGGGATGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAg                                                                   <  5:129192/135‑1 (MQ=255)
                                         cTTTCCAGCCGGCGCTGCAAGGGCGCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACGTGGCGCAGCAGGGCGTAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                     <  1:312831/133‑1 (MQ=255)
                                           ttCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGAGTGAAGTg                                                                 >  1:144255/1‑135 (MQ=255)
                                               agctgGGGCTGCAAGTGCCCCTGGGGCTCGGCGTGGTGCCCCCGCCGGGGGTGGGGGCGCACCGGGGGCTGCCGGGCGCTTGGTGGGGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                             <  4:108401/135‑1 (MQ=255)
                                                 ctggCGCTGAAAGTGCTCCAGGCGCGCGGCGGGGTTCAGCCGCCGGGGCAGCGGGCGAACCTGGCGCAGCCGGGCGTAAGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                           <  3:100001/133‑1 (MQ=255)
                                                 cgggggcTGCAAGTGCGCCCGGCGCGCGGCCTGGTTCCGCAGCAGGCGCAGGGGGCGCACCTGGCGCAGCTGGGCGTATGGGGGCGGGGGGGGGGAGGGTGTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                           <  2:76473/130‑1 (MQ=255)
                                                 ccggggcTGCAAGGGCGCCCGGCGGGCGGCGGGGTGCCGCAGCAGGCGCTGGGGGCGCACCTGGCGCAGCAGGGGGGAAGGTGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                           <  8:160963/130‑1 (MQ=255)
                                                 cagGCGCGGCAAGTGCGCCCGGCGCGCGGCCTGGTTCCGCAGCCGGCGCCGGGGGGGCACCGGGCGCTGCTGGGGGGATGGGGGGGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                           <  8:314320/135‑1 (MQ=255)
                                                           aaGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTa                                                 >  2:295987/1‑135 (MQ=255)
                                                                   cAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTcc                                         >  4:91190/1‑135 (MQ=255)
                                                                     ggCGCTCGGCCGGGTTCAGCAGCAGGCGCAGGGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGCGGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGt                                       <  5:255369/135‑1 (MQ=255)
                                                                       cgcTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTg                                     >  1:47102/1‑135 (MQ=255)
                                                                             gcgtGGTTCCGCAGCAGGCGCATGGGGCCCACCTGGCGAAGCTGGGCGGAAGGTGGCGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCtgtg                               <  8:329153/132‑1 (MQ=255)
                                                                                      cgcagctgGCCCAGCGGGCGCCCGTGGCGACGCCGGGGGGAAGGGGGCGGGGGGGGGGCGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCctct                      <  1:39173/134‑1 (MQ=255)
                                                                                                   gggggCGCACCGGGCGCTGCAGGGCGGATGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc         <  4:117770/133‑1 (MQ=255)
                                                                                                   gCGGGCGCACGGGGGGCAGCTGGGCGTATGGTGGCGGGGCGGGGGCGGGTGTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc         <  6:147684/135‑1 (MQ=255)
                                                                                                   gCGGGCGCACCGGGCGCCGCAGGGCGTATGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc         <  5:136275/135‑1 (MQ=255)
                                                                                                         gcACCGGGCGCAGCCGGGGGGAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTcc   <  7:159706/135‑1 (MQ=255)
                                                                                                          cACCTGGGGCAGGCGGGCGCTAGGGGGGGGGGGGGGGTAGGGGGTGAAGGCCATGTGGGTACGCGTGAATTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt  <  3:77236/135‑1 (MQ=25)
                                                                                                                                      |                                                                                                          
ACGCCACATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCT  >  KT2440/1524598‑1524838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑