Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,720,9700AC40.9% 29.0 / 10.8 44F84V (TTC→GTC) plsBGlycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (18/8);  new base C (1/17);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 2.94e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAG  >  KT2440/1720840‑1721091
                                                                                                                                  |                                                                                                                         
cTGCCAGGAAGGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCCGGCGCGCGAGGGTGGGCGGGGCGCCGGTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTCCGGTAGAAGACcgcg                                                                                                                       <  2:197432/135‑1 (MQ=255)
cTGACTGGATTGAGGTGGGCATCTTCTTCGGCAGGTGGGGTGCCGGCAGCCACCTGGCGGTCGTGCGGGGGCGGGGCGCGGCTTTGGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCTGTTATAACACcgcg                                                                                                                       <  6:176980/135‑1 (MQ=255)
cTGACAGGATTGATCTGCGCTTCTGCTTCGGCATGCTGGGTGACGGCAGCCACCAGGGGCTCGAGCGGGGGGGGGGCGCGGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTAAGGTAGAAGAAcgcg                                                                                                                       <  8:57101/135‑1 (MQ=255)
cTGACAGGAATGATCTGCGCATCTTCTTCGGCTGGCTGGCTGCCGGCAGCCACCCGGCGCTCGCGCGGGGGGGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGGCGGGGTCGGGGGTCAGGTATAAGACcgcg                                                                                                                       <  6:293825/135‑1 (MQ=255)
cTGACAGGAATGAGCTGCGCATGTTCTGCGGGTGGGGGGCTGACGGCAGCCACCTGGCGCTCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAAcgcg                                                                                                                       <  5:326232/135‑1 (MQ=255)
cTGACAGGAATGAGCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTAGAAGAAcgcg                                                                                                                       <  7:193291/135‑1 (MQ=255)
cTGACAGGAATGAGCGGCGCATCTTCTGCGGCAGGCTGGCTGACGGCAGCCACCCGGCGCGCGCGCGGGGGCGGGGCGCCGCTTTTGCCCTGGCGGCCGAGCCAGGCCGGGGCGGGGGGCAGGTAGAAGACcgcg                                                                                                                       <  6:136727/135‑1 (MQ=255)
  gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACgcggc                                                                                                                     >  2:428/1‑135 (MQ=255)
             tCTGCGCAGCTTCTTCGGCAGGCTGGCTGACGCCAGCCACCTGGCGCGCGCGCGGGGGCGGGGCGCCGCTTTTGGCCGGGCGGCCGAGCCAGGCGGGGCCGGGGGTCTTGTAGAAGAACGCGGCGGGTTCCTGc                                                                                                           <  8:109044/134‑1 (MQ=255)
                gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTg                                                                                                       >  7:106956/1‑135 (MQ=255)
                gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTg                                                                                                       >  7:52724/1‑135 (MQ=255)
                gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCATTTTTCCTCGCGCACGAGCCACTCCCCGTCCCGGGGGACGTATTACACCGCGGCGGGTTCCTGCACTg                                                                                                       >  2:193167/1‑135 (MQ=255)
                  gcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTggg                                                                                                     >  3:155039/1‑135 (MQ=255)
                   cATCTTCTTCGGCATGCTGGCGGACGGCAGCCACCCGGCGCTCGCGCGGGGGCGGGGCGCCGCTTTTGTCCGGGCGGCCGAGCCAGTCCGGGGCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGc                                                                                                    <  8:42525/135‑1 (MQ=255)
                    aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGTCGGCCGAGCCCGTCCGGGTCGGGGGGGAGGGATTAGAACGCGGGGGGGTCCTGCAGTGGGcc                                                                                                   >  1:164719/1‑135 (MQ=255)
                    aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTCCTGGCGGCCGCGCCAGTCCGGGTCGGGGGGCAGGTAGCAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                                   >  3:295074/1‑135 (MQ=255)
                      ctgcttcGGCAGGCGGGCGGACGGCAGCCACCAGGCGCGCGAGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGa                                                                                                 <  7:225736/132‑1 (MQ=255)
                         cttcGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGCCgg                                                                                              >  5:302161/1‑135 (MQ=255)
                          ttcGGCAGGCGGGCGGACGGCCGCCACCAGGCGCGCGAGCGGGGGCGGGGCGCCGCTTTGGGCCGGGCGGCCGAGCCCGGCCGGGGCGGGGGGCCGGTACAAGACCGCGGCGGGTTCCTGCAGTGGGCCGa                                                                                                 <  5:142426/131‑1 (MQ=255)
                                   cgggcggACGGCAGCCACCCGGCGCGCGCGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGCCAGGTACAACACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGgcag                                                                                    <  2:249363/133‑1 (MQ=255)
                                       ctgCCGGCAGCCACCAGCCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCGTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTTCAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCAcc                                                                                >  7:38212/1‑135 (MQ=255)
                                         gACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTGGGGGGTCAGGGAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCgg                                                                              >  7:206049/1‑135 (MQ=255)
                                            ggCAGCCACCCGGCGCTCGCGCGTGGGCGGGGCGCCGGTTTTGTCCGGGCGGCCGAGCCAGTCCGGGTCGGGGGGCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGgcg                                                                           <  4:128993/135‑1 (MQ=255)
                                              cAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTTTCCTGGCGGGCGCGCCCGTCCGGGTCGGGGGGCAGGTAGAAGAAGGCGGCGGGGTCCTGCAGTGGGCCGCCGGCTCCCGGCCGCCCCGGGcgcg                                                                         >  8:126826/1‑135 (MQ=255)
                                               aGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGTCCGGGTCGGGGGTCCGGGAGAAGAAAGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCCGCCCCGGGCGCgg                                                                        >  5:76199/1‑135 (MQ=255)
                                                   accaGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTCCCGGCCGCACCGGGCGCGGCAGc                                                                    >  7:77808/1‑135 (MQ=255)
                                                    cccggCGCTCGCGGGGGGGGGGGGCGCGGCTTTTGTCGGGGGGGCCCAGCCAGGCGGGGGCGGGGGCCTGGAAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGcc                                                                   <  3:281954/132‑1 (MQ=255)
                                                    ccaGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTTTGCTGGCGGCCGCGCCAGTCCGGGTCGGGGGGCAGGGAGAAGAACGCGGCGGGTTACATCAGTGGGCCGCCGGCGTCCCGCCGCCCCCGGCGCGGccccc                                                                   >  4:12266/1‑131 (MQ=255)
                                                       ggCGCGCGCGCGGGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGGCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCg                                                                <  4:155039/135‑1 (MQ=255)
                                                         cgcTCGAGCGGGGGCGGGGGGCCGCTTTTGTCCGGGCGGCCGAGCAAGTCCGGGGCGGGGGGCAGGTAGAACACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGcc                                                              <  8:9845/135‑1 (MQ=255)
                                                                      ggggggcgCCGCTTTTGTCCGGGCGGCCGCGCCTGGCCGGGGCGGGGGTCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGt                                                 <  3:64764/133‑1 (MQ=255)
                                                                      gcggggcgCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTAGGGGGGCAGGTAGAAGAACGCGGCGGGTTCCTGCCGTGGGCCGCCGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCGTGGTGCCCTCGt                                                 >  3:283174/1‑135 (MQ=255)
                                                                                        ccTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCACCGCCTTGGTGCGCTCCTGGTCGAGACCGGCCAGGt                               >  6:315574/1‑135 (MQ=255)
                                                                                              ggcCGAGCCAGGCGGGGGGGGGGGTCAGGTAGAAGCACGCGGCGGGGTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGa                         <  3:218297/135‑1 (MQ=255)
                                                                                              gcccGAGCCCGCCGGGGGGGGGCGCCAGGTACAAGCCCGCGGCGGGATCCTGCAGTGGGCCGACGGCTACCGGCAGAACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGa                         <  2:100967/133‑1 (MQ=255)
                                                                                                    gCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCgg                   >  4:64398/1‑135 (MQ=255)
                                                                                                          cgggggCGGGGGGCCGGTAGAACACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGa               <  8:16923/131‑1 (MQ=255)
                                                                                                          ccGGGTCGGGGGTCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGGCGGTGAGCGCGGGCGAgg             <  4:40233/135‑1 (MQ=255)
                                                                                                          ccGGGGGGGGGGGCAGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAgg             <  1:17018/135‑1 (MQ=255)
                                                                                                          ccGGGGCGGGGGTCCGGTAGAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGGCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAgg             <  2:19070/135‑1 (MQ=255)
                                                                                                          ccGGGGCGGGGGCCTGGACGAATCCCGCGGCGGGGTCCTGCTGGGGGCCGTCGGCAACCGGCACCACCGGGCGCGGCAGCCCCGCCTTGGCGCACTCGTGGTCGAGCACGGCCAGGGCGGGGAGCGCGGGCGAgg             <  6:248205/135‑1 (MQ=255)
                                                                                                            gggTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGc           <  8:106956/135‑1 (MQ=255)
                                                                                                                 gggggTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGc      >  6:165767/1‑135 (MQ=255)
                                                                                                                     gTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGCGGGCAACGCATAg  >  6:90120/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                         
CTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTAGAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGGGCAACGCATAG  >  KT2440/1720840‑1721091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑