Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,308,3210CG25.0% 111.1 / 10.4 48A230P (GCC→CCC) fucAL‑fuculose phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (4/32);  new base G (12/0);  total (16/32)
Fisher's exact test for biased strand distribution p-value = 2.61e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGC  >  KT2440/2308188‑2308451
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gCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGcc                                                                                                                                   <  6:10738/135‑1 (MQ=255)
gCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGcc                                                                                                                                   <  6:167643/135‑1 (MQ=255)
     tttGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCttt                                                                                                                              <  8:226926/135‑1 (MQ=255)
     tttGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCttt                                                                                                                              <  3:33007/135‑1 (MQ=255)
        gCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCACGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGc                                                                                                                            <  4:259139/134‑1 (MQ=255)
        gCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGc                                                                                                                            <  6:304611/134‑1 (MQ=255)
        gCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGc                                                                                                                            <  8:4094/134‑1 (MQ=255)
         ccGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTCTTTGgcc                                                                                                                          >  3:18467/1‑135 (MQ=255)
         ccGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGcc                                                                                                                           <  4:279278/134‑1 (MQ=255)
          cGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCa                                                                                                                         <  7:167158/135‑1 (MQ=255)
                   ggAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCgg                                                                                                                <  7:26735/135‑1 (MQ=255)
                   ggAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCgg                                                                                                                <  7:226692/135‑1 (MQ=255)
                        gagTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATg                                                                                                           <  7:274257/135‑1 (MQ=255)
                            aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCGTTGGCCCGTGCTGGGGGGTTggggg                                                                                                       >  6:1922/1‑134 (MQ=255)
                                 ttCGATCAGCCCAGGCAGTCGCCATGGGCACCCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTATGGGGGTGGAAATCCAGTCGTGGGGCGCTTTCCGAACTGCTTAGGGGCGGGGGCCGATg                                                                                                  >  1:27481/1‑135 (MQ=255)
                                   cGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTGCCCCGTGGGTCGGGGATGGGGCGGTTGTc                                                                                                >  4:117054/1‑135 (MQ=255)
                                     aTCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCAc                                                                                              <  4:294479/135‑1 (MQ=255)
                                      tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGGGTTGGCCAGGGGGTCGGGGATGGGGCGGGTgggacc                                                                                             >  8:144735/1‑130 (MQ=255)
                                      tCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCTTTGCCCAGTGCGTCGGGGCTGGGGCGGATGTGAcc                                                                                             >  4:65636/1‑135 (MQ=255)
                                        aGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCttt                                                                                                                              >  4:85011/1‑100 (MQ=255)
                                        aGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCttt                                                                                                                              <  3:85011/100‑1 (MQ=255)
                                          cccAGGCAGTAGCCCTGGGCACGCAATGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGACCAGTGCTTCGGGGATGGGGCGGATGTCACCGgcg                                                                                         <  6:301390/135‑1 (MQ=255)
                                                  gTCGCCATGGGCTCGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGGGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGc                                                                                 <  6:320863/135‑1 (MQ=255)
                                                      ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagc                                                                             <  3:270428/135‑1 (MQ=255)
                                                      ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagc                                                                             <  7:146696/135‑1 (MQ=255)
                                                       cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCGTTGGCCCGGGGGTCGGGGGTGGGGCGGCGGTCACCCGCGCCCCAGGCccgcc                                                                            >  6:302817/1‑134 (MQ=255)
                                                       cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCGTTGCCCACGGGTTCGGGGGTGGGGGGGCGGTGCACGGCGCCCAGGGCccgca                                                                            >  1:117233/1‑135 (MQ=255)
                                                        aTGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagcag                                                                           <  6:18147/135‑1 (MQ=255)
                                                            gCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCGTTGGGCAGTGCGTGGGGGGTGGGGCGGAGGTACCCGGCGCCCAACGGCAGCAGtggg                                                                       >  7:177490/1‑132 (MQ=255)
                                                            gCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTGCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTg                                                                        <  2:131508/134‑1 (MQ=255)
                                                              aCGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGGGCTTTGGCCAGTGCGTAGGGGGTGGGGGGGTGGTCACCGGCGCCCCTGGCCCGCAGTTGCAt                                                                     >  4:270428/1‑135 (MQ=255)
                                                                   aGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCTTTGGCCAGTGGTTAGGGGATGGGGGGGATGGCACCGGCGCCCCAGGCCCGCAGGTGGATCattt                                                                >  4:108772/1‑134 (MQ=255)
                                                                        cGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGTAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                           <  8:107638/135‑1 (MQ=255)
                                                                        cGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTGTGGGGGTGGAAATCCAGTCGTGGGCCGCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                           <  8:181087/135‑1 (MQ=255)
                                                                             cgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCGGCGTTTGCCAGTGGATGGGGGAAGGGGGGGTGGTCACCGGCCCCCAAGGGCAGCAGGTGGCGCGTTGATGCGCGCTc                                                      >  6:164488/1‑135 (MQ=255)
                                                                              gcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTCTGTGGCCCGGGGTTGGGGGGTGGGGCGGCTGGCCCCCGCGCCCCAGGCCCGCCGTGGCGTCTTTGGTtcgcgcgc                                                      >  6:248974/1‑132 (MQ=255)
                                                                                       cTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCaga                                            <  3:96419/135‑1 (MQ=255)
                                                                                             gcagcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAgagcgagc                                      <  3:854/135‑1 (MQ=255)
                                                                                               agcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTCGTTGGCCCCGGGGTCGGGGCTGGGGGGGGGGTCCCCCGCGCCCAGGGCCAGCAGTTGCATCTTTGCTGCGCCCGCCAACACCAGAGCGCGCAc                                    >  2:31152/1‑135 (MQ=255)
                                                                                               agcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGGGTTGCCCAGGGCGTCGGGGATGGGGGGGATGTCCCCGGCGCCCCGGGCCCGCCGGTGGCGGTTTGGTGCGCGCGCGCACCGCAgcgcgcgccc                                    >  4:230070/1‑133 (MQ=255)
                                                                                                 caCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGc                                  <  3:165147/135‑1 (MQ=255)
                                                                                                       gACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCtt                            <  3:39498/135‑1 (MQ=255)
                                                                                                                   tGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAAc                <  3:84621/135‑1 (MQ=255)
                                                                                                                     gAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGc              <  1:133119/135‑1 (MQ=255)
                                                                                                                             gtaggcggCATCTTTGCCCAGTGCGTCGGGGATGGGGCGGATGGCACCGTCTCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGCACGCCCAGCGAt      <  6:336972/129‑1 (MQ=255)
                                                                                                                             gTCGTGGGCCTCTTTGCCGAGTGCTTCGGGGAGGGGGCGGATGTCACCGGCGCGCATGGCCAGCAGTAGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCGTCCTCGATCGAACGCCCAGCGAt      <  4:113369/135‑1 (MQ=255)
                                                                                                                             gTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt      <  1:36806/135‑1 (MQ=255)
                                                                                                                             gTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt      <  1:31152/135‑1 (MQ=255)
                                                                                                                              tCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATc     <  5:277728/135‑1 (MQ=255)
                                                                                                                                 tGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATcagc  <  7:123568/135‑1 (MQ=255)
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GCACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTCTTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGC  >  KT2440/2308188‑2308451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑