| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,496,789 | 0 | A | C | 55.0% | 0.6 / 11.6 | 20 | intergenic (+91/‑142) | speB/CJ019_02354 | Agmatinase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/4); new base C (11/0); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGTATGCGGCGCGGCACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCGAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCTATCAAACCCACCAGGCGCGG > KT2440/2496660‑2496907 | cTGTATGCGGCGCGGCACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGAGCGTGGAGCGGGCGCGCCCGCGACTGGc > 1:261089/1‑135 (MQ=255)cTGTATGCGGCGCGGCACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCGACTGGc > 2:127960/1‑135 (MQ=255) gcggcgcggcACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCCCAGGGCCAGAAATCTGGGGGTGACCGTGGAGCGGGCGCGCCCGCGCCTGGCTGcgca > 7:32167/1‑132 (MQ=255) cggcgcggcACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGCAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCCCGCCTGGCTGCCAag > 3:262908/1‑135 (MQ=255) ggcACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCCCAGGGCCAGAAATCTGTGGGGGCCCGTGGAGCGGGGGGCCCCGCGCATGGGTTCGAAGCAcccc > 6:194731/1‑135 (MQ=255) ggcACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGCCCGTGGAGCGGGCGCGGCCGCGCCTGGCTGGCAAGCCGccc > 1:54066/1‑135 (MQ=255) ggACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGGGCGCCCGCGCCGGGCTGCCACGCCGCccgccgccgggc > 1:222083/1‑129 (MQ=255) aCGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACCCGTGTCTCACAGGGCCAGAAATCTGTGGGTGACAGTGGAGCTGGCGCGCCCGCGCATGGCTGCAAAGCAGCCCTCCCAGGCCCATAc > 6:261020/1‑134 (MQ=255) aCGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCGCATGGCTGCCAAGCCGCCCTCCGCGGCCCAAAc > 4:126569/1‑134 (MQ=255) aCGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCCCCCAATGCAGCAAACCAGCCCTCCGAGGCCCAGAc > 4:317368/1‑134 (MQ=255) gTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCCCCTGGCGGCAAAGCCGCCCTCCCCGGCCCCCACTGCAACa > 3:137289/1‑134 (MQ=255) cGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCGCCTGGCTGCACAGCCGCCCGCCGCGGCCCCTACGGGACCCTCAgggagcgt > 3:226236/1‑129 (MQ=255) atccgcgGGCAAGCCCGCGCCCACCCGTGGCTCACTGGGCCACAACTCTGGGGGTCACCGTGGAGGGGGCGCGCCCGCGAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCa < 8:324811/132‑1 (MQ=255) caccCGTGTCTCACAGGGCCAGAAATCTGTGGGTGCCCGTGGAGCGGGCGCGCCCCCGCCTGGCAGCCAAGCCGCCCGCCGAGGGCCACTAGGAAACATCAGGGATCAACTCCCCCCGCATTGACACattttagg > 8:43292/1‑131 (MQ=255) tcACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCGAATGGCGGCAAAGCCGCCCTCCGAGGCCCATACTGAAACATCAGGCATCATCTCCACCCGCCTTCCCACTTTTGCCGCGCAccccgc > 8:100786/1‑133 (MQ=255) aagcTGGGGGGGCCCGGGGCGGGGGGGCGCCCGCGAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCTa < 4:137289/132‑1 (MQ=255) gggTGACCGTGGAGCGGGCGCGCCCGCGAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCGATCAAccc > 6:69777/1‑135 (MQ=255) gggTGACCGTGGAGCGGGCGCGCCCGCGAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCGATCAAAcc > 6:69774/1‑135 (MQ=255) gggcggggggGCCCCCGCTAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCTATCAAACCCACCAGgcg < 8:32167/129‑1 (MQ=255) ggCGGGGGCCCCGCCGAATGGATGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCTATCAAACCCACCAGGCGCgg < 8:148232/134‑1 (MQ=255) | CTGTATGCGGCGCGGCACAAGGTGGACAACGGCCAGTAGATTTTGCGGCCATTCGCGGGCAAGCCCGCTCCCACACGTGTCTCACAGGGCCAGAAATCTGTGGGTGACCGTGGAGCGGGCGCGCCCGCGAATGGCTGCAAAGCAGCCCTCCGAGGCCCATACTGAAACATCAGGAATCATCTCCACCCGCATTCACACTTTTGCCGCGAACCCCTCGCGCCTTAGGCTATCAAACCCACCAGGCGCGG > KT2440/2496660‑2496907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |