Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,138,3670TG26.5% 57.6 / 11.8 34F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (3/22);  new base G (9/0);  total (12/22)
Fisher's exact test for biased strand distribution p-value = 4.19e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  KT2440/3138238‑3138499
                                                                                                                                 |                                                                                                                                    
acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGt                                                                                                                                 >  3:135733/1‑135 (MQ=255)
     aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggc                                                                                                                            >  8:282730/1‑135 (MQ=255)
     aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgggg                                                                                                                            >  3:34332/1‑134 (MQ=255)
     aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTGTGTTcggg                                                                                                                            >  8:150810/1‑134 (MQ=255)
     aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTaggc                                                                                                                            >  2:278227/1‑135 (MQ=255)
        cGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCCGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTGTGTTGgggggc                                                                                                                         >  7:309152/1‑135 (MQ=255)
            cTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGGGGCCggg                                                                                                                     >  3:256883/1‑135 (MQ=255)
                        gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCTGGGTTAGCGgcagc                                                                                                         >  6:135492/1‑135 (MQ=255)
                          cATCGGATAATCCCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                       <  8:270330/135‑1 (MQ=255)
                              ggAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGGTGAGCGGCAGCcccacc                                                                                                   >  7:117887/1‑135 (MQ=255)
                                   aTACCCGGAGGCGGCTAATGCCGCTGGCCTGGCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTc                                                                                              <  2:248558/135‑1 (MQ=255)
                                     aCCCGGAGGAGGCTAATGCCGCTGGCCTGTCGGTGCGGGGCTACGGGTTCGCCAGCTACCTGATCAGCGGGCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                            <  7:86191/135‑1 (MQ=255)
                                             gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg                                                                                    <  2:61972/135‑1 (MQ=255)
                                               ggcTAATGCCGCGGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgct                                                                                  <  3:286527/135‑1 (MQ=255)
                                                 cTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgctgc                                                                                <  5:122252/135‑1 (MQ=255)
                                                      gCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTcc                                                                           <  7:30164/135‑1 (MQ=255)
                                                      gCCGCGGGCCTGTCGGTGCGGGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTcc                                                                           <  3:314291/135‑1 (MQ=255)
                                                            ggCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCTGGTTCAGCGGCAGCCCCACCGGTTGCGGCGAGATGCTGCTGTCCgtggtg                                                                     >  4:127735/1‑135 (MQ=255)
                                                                           ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCtt                                                      <  7:110711/135‑1 (MQ=255)
                                                                           ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCtt                                                      <  3:194262/135‑1 (MQ=255)
                                                                           ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCtt                                                      <  7:282730/135‑1 (MQ=255)
                                                                               aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGCCGGGGTAAGCGGCCGCCCCACCGGGTCCGGCGAGATGCTGGTGTGCGTGGTGGGCATCGCTTTTGTTgggg                                                  >  4:140557/1‑135 (MQ=255)
                                                                               aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGGTTTTGGGGGGGCGGGGTGAGCGGCCGCCCCACCCGGTCGGGGGCGCTGCGGCTGGCGGGGGGGGCGCGCGCGTTTGTTgggg                                                  >  7:87310/1‑135 (MQ=255)
                                                                                         cAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTtctc                                        <  8:99538/135‑1 (MQ=255)
                                                                                             tACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAg                                    <  2:26071/135‑1 (MQ=255)
                                                                                                    tCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTg                              <  8:86837/134‑1 (MQ=255)
                                                                                                                gCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCAt                   <  6:331586/133‑1 (MQ=255)
                                                                                                                gCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCAt                   <  1:201326/133‑1 (MQ=255)
                                                                                                                  ggCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCTTCTGCAGCCGAGCATcgg                <  7:200221/134‑1 (MQ=255)
                                                                                                                   gCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg              <  4:34332/135‑1 (MQ=255)
                                                                                                                    cAGCCTGGCCGCGTTTTGTGCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcgg             <  1:278227/135‑1 (MQ=255)
                                                                                                                      gCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCa           <  1:254582/135‑1 (MQ=255)
                                                                                                                            ccGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACgct       <  7:223424/133‑1 (MQ=255)
                                                                                                                               cgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  1:143369/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
ACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  KT2440/3138238‑3138499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑