Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,491,3370GC40.6% 29.0 / 13.1 32intergenic (‑699/+22)CJ019_03306/CJ019_03307hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (6/13);  new base C (13/0);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 1.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTGACCCAG  >  KT2440/3491213‑3491470
                                                                                                                             |                                                                                                                                        
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCCCCGTCACAGCCGCAACCGCATTCGCGGGCAGCCTGCCCGCGAACACCCGCGCA‑Gcacg                                                                                                                               >  6:135404/1‑132 (MQ=255)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGGCGCCCGCCCCCCCACACCCGCGCC‑ACCgg                                                                                                                               >  6:317531/1‑135 (MQ=18)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGCACACCCCCgcc‑gcccc                                                                                                                               >  3:226774/1‑133 (MQ=21)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCAGCCCGCGTACACCGGCGCC‑GCCgg                                                                                                                               >  2:50024/1‑135 (MQ=255)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGCACACCGGCgcc‑gcccg                                                                                                                               >  8:244348/1‑133 (MQ=255)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCTGCCCGCGCACACCCGCCCA‑Gcccg                                                                                                                               >  2:23753/1‑133 (MQ=21)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCCGGCAGCCGGCCCCCGAACACCCGCGCA‑GCCgg                                                                                                                               >  4:61699/1‑135 (MQ=255)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCCCCGGGCGCCGGGCCGCCCCCACCCGCgcc‑gcccc                                                                                                                               >  5:235384/1‑133 (MQ=18)
ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGAGAATAGCCCGGACATGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCACCCTCCCCGCCAACACCGGCgcc‑gcccg                                                                                                                               >  4:159262/1‑133 (MQ=21)
ggtggtGTCGCCACGTTGTAGGAGCTGGTATACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGCCAGCCCTCCCGCGAACCCCCGCGCC‑ACCgg                                                                                                                               >  4:125028/1‑135 (MQ=21)
        cGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCCGCGCC‑GCCGGGTTCATc                                                                                                                        >  2:225191/1‑134 (MQ=255)
          ccACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCCCAGCCGCCACCGCATTCCCGCGCACCCTTCCCCCGACCACCGCCGCA‑CCCGGgttgcgacc                                                                                                                      >  7:33805/1‑128 (MQ=17)
               ttGTAGGAGCGGGTTTACCCGCTAATAGGCCGCA‑CGGCCCGCCAAATAACCGGGTAATTTCACCGTCCCCCCCGCCCCCGCATGCGGGGGCCGCGTGCCGGCTAACACCGGCGCA‑GCCGGTTTCATCCAACTc                                                                                                                  <  2:43046/133‑1 (MQ=255)
                       gCTGGTTACCCGGCGATTAGGCGGGACCTGCGGGCCACTAGCCGTGGTCA‑TCCACGGTCACAGCCGCCCCCGCATTCGGGGCCCGCCGGCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATc                                                                                                        <  2:30454/135‑1 (MQ=255)
                           gTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCCCGAACACCCGCGCA‑GCCCGTTTAATACAAATAAACCACCATAGAcc                                                                                                    >  6:164017/1‑135 (MQ=18)
                           gTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCGGCCCGCGCACACCCGCGCC‑GCCGGGTTGATCCAACTCAACGCCCAACGAcc                                                                                                    >  8:151981/1‑135 (MQ=21)
                           gTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCCGGCCGCCTGCCCCCGGACACCCGCGCA‑GCCGGGTTGATCCAACAAaaagaccaaagacc                                                                                                    >  3:233521/1‑135 (MQ=18)
                                        ttggccggccGTGCCGGCCAATACCCCTGGTAA‑TTCCCGGTCCCCCCCGCACCCGCTTGCGCGGGCAGCCTCCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACg                                                                                       <  7:150070/132‑1 (MQ=255)
                                                 ccggCCGGCCACTATCCCTGGTCA‑TTCCCCGTCACAGCCGCCACCGCATGCGCGGGCCGCCGGCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCAt                                                                             <  6:105869/132‑1 (MQ=255)
                                                 ccTGCCGGCAACTAGCCGGGGCCA‑TCCCCCGCCACCCCCCCCCCCGAATTCGCGGGCCGCCGCCCCGCCAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCAt                                                                             <  1:23753/135‑1 (MQ=255)
                                                      cGGCCACTAGCCCTGGTCA‑TGCCCCGCCACCCCCCCCCCCCCATGCGCGGCCCGCCGCCCGGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGAtt                                                                        <  1:225191/135‑1 (MQ=255)
                                                                       cA‑TACACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAAtt                                                      <  4:103404/135‑1 (MQ=255)
                                                                        a‑TTCACTGTCACAGCCCCCACCCCATTCGGGGGAAGCGTGCCCGGCAACACCGGCGCA‑GCGGGTTTCATCCAACTCAACTACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTg                                                     <  3:159262/135‑1 (MQ=255)
                                                                        a‑TTCACCGTCACAGCCGCCACCGCATTCGCCGGCAGCCAGCCCGCGCAAACCCGCGCACCCCGGTTTC‑TACAACAAAAAGCCCCACCCCCAGTGCG‑CAAACGGATCTGCCATAGCATG‑GCATGGATGGACAAttt                                                     >  6:40242/1‑134 (MQ=255)
                                                                             aCCGTCACAGCCGCCACCGCATTCGCCGGCAGCCGGCCCCCCCACACCCGCGCC‑GCCGGTGTAATCCCACTACACCCCCAGAGCCCTGTTCGCCAAACG‑CTCTGGCATCGATTGCCCTGGCAGGCCCAAtttcgt                                                  >  4:110963/1‑131 (MQ=255)
                                                                                                   tCGCGGGCAGCCTGCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGaa                           <  4:233521/135‑1 (MQ=255)
                                                                                                           tgcgggCCGGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACAccc                   <  7:175259/130‑1 (MQ=255)
                                                                                                           aGCCGGCCCGCTAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACAccc                   <  5:164017/135‑1 (MQ=255)
                                                                                                                gCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGACATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGGTTGAAGCACCCCCggtac              >  5:28914/1‑131 (MQ=255)
                                                                                                                 cccGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CACACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCAt             <  2:210255/135‑1 (MQ=255)
                                                                                                                    gcgAACAACGGCGCA‑GCCGGTTTCATCCAACTCAAAGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGt          >  4:137899/1‑135 (MQ=255)
                                                                                                                            cGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTGACCCAg  <  4:294791/135‑1 (MQ=255)
                                                                                                                             |                                                                                                                                        
GGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCA‑TTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGAACACCGGCGCA‑GCCGGTTTCATCCAACTCAACGACCATCGACCTGTACG‑CAAACGTCTCTGCCATCGATTG‑CCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCACACCCGTTCATCGTTGACCCAG  >  KT2440/3491213‑3491470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑