Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1450AC56.4% ‑4.6 / 15.8 46L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (6/11);  new base C (13/9);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245016‑4245265
                                                                                                                                  |                                                                                                                        
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGGCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCCGCGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCTGACCAACCCGGCGGCCCGGgcg                                                                                                                     >  6:107173/1‑135 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCTGACCAACCCGGCGGCCCGGgcg                                                                                                                     >  7:106255/1‑135 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCAACCCGGCGGCCCGGgcg                                                                                                                     >  2:35307/1‑135 (MQ=255)
    cgcgTATCAGCCGCTTGGTCTGCGGATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCCGGCAACGGCTCGTCGGCCCAGCCTTCGCTGACCAACCCCGCGGCCCGGGGGCGAt                                                                                                                 >  8:109105/1‑135 (MQ=255)
                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCACGGCGCGATCCGCCGCCCACAAcac                                                                                                 >  4:187742/1‑133 (MQ=255)
                           gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCAGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGATCGGCGCCCCACAACTCGGCGacg                                                                                          >  3:39658/1‑133 (MQ=255)
                               cggCGAAGGGCGGGCCATTGGCCTGGCGCCGGGCCAGTGCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  7:99490/134‑1 (MQ=255)
                                  cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGCTCGGCGCCCCCCCACTCCGCGAGGacccgcc                                                                                   >  7:50670/1‑129 (MQ=255)
                                    aggggcGGGCAATTGGCCAGGCGCCGGGCCAGTGGGGCGGCAACGGCAGGCAACGGAGGGGCGGCGAATAATGCGCTCACCAACCCGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  2:69538/130‑1 (MQ=255)
                                         cGGCCCATTGGCCTGGCGCCCGGACAGTTGGGCGGAAACGGCAGGCAACTGCTCGGCGGCGCATACTTCGCGGACCAACCCGGCGGCCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                            <  6:139921/135‑1 (MQ=255)
                                            gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGCTCGGCGCCCCCCCACGCGGCGAGGGACACCAGgcgcgttgc                                                                         >  3:267271/1‑130 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCACCCGGCGGCCCGGGCGCGCGCGGCGCCCCCCACCTCGGGGGCGGACCGCAGGCGCCTGGccg                                                                       >  6:139527/1‑134 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACGCCGCGAGGAAAACCCGGCGCGTTGCCt                                                                       >  2:27266/1‑135 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGAAAGGCGCCCCCCCACTCGGCGCGGAACACCCCGCGCGTGGCCt                                                                       >  1:189099/1‑135 (MQ=255)
                                                   ccctggCGCCGGGCCAGTTCGGCGGCAAGGGCAGGAAACTGCGCGTCGGCGCAGACTTCGCTCACAAACCCGGCGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  3:288872/131‑1 (MQ=255)
                                                   ccccggCGCCGGGCCTGGGCGGCGGCACGGGCCGCCAGCGGCCCGGCGGCGCAGAGTGCGCTCCCAACCCCGGCGCCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  2:282034/131‑1 (MQ=255)
                                                        gcgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTGGGCGGCGCAGACTTCGCTGACCACCCCGGCGGCCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  6:43702/135‑1 (MQ=255)
                                                        gcgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  7:197677/135‑1 (MQ=255)
                                                        gcgcCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGCCCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  8:50670/135‑1 (MQ=255)
                                                        gcgcCCGGCATGTGCGGCGGAACCGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCGCACCACCCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  4:135133/135‑1 (MQ=255)
                                                           cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACGCGGCGAGGAACAGCAGGCGCGTGGCCTGCGCGCAGCCGa                                                           >  8:226363/1‑134 (MQ=255)
                                                              ggCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCCCGGCGCGCTCGGCGCCCCCCCACACGGCGAGGCAAAGCACGCGCTTGGCCAGCGCCCAGCCgcgccg                                                       >  6:63888/1‑130 (MQ=255)
                                                                  cgtgcggcggCAACGGCAGCCAACGGCGCGGCGGCGCAGACTGCGCTGACCAACCCGGCGGCCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  6:130369/131‑1 (MQ=255)
                                                                  actgcggcggCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  4:111858/131‑1 (MQ=255)
                                                                             aaCGCCAGGAAACTCCGGGTCGGCGCAGACTTCGCTCCCAAACCCGGGGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                        <  7:200070/135‑1 (MQ=255)
                                                                                 gccggcaACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTgg                                   <  2:37703/132‑1 (MQ=255)
                                                                                 gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGTGGCAGGTGCCCGCTg                                    >  8:202906/1‑135 (MQ=255)
                                                                                        aCTGCGCGGCGCCCCAGACTGCGCTCCACACCCCGGCGCCCGGGGCGCGCGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                             <  6:1341/135‑1 (MQ=255)
                                                                                          tGCTCGTCGGCGCAGACTGCGCTGCCCAACCCGGCGCCCAGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:162485/135‑1 (MQ=255)
                                                                                          tGCGCGGCGGCGCAGACTGCGCCCACCACCCCGGCGCCCCGGCCGCGACCGCCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:308195/135‑1 (MQ=255)
                                                                                          gggtcggcgGCGCCCACTGCGCTGACCACCCCGGCGGCCCGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTGGGCGTGCTCGCTGCCGATCAGCCGGGGCAGGTGCCCGCTGGCGCCGGCa                           <  2:38065/132‑1 (MQ=255)
                                                                                               gtcgGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCCCAACGCGGCGAGGGACAGCAGGCGCTTGGCCGGCTCGCGGCCGCTCCGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCggg                      >  4:112908/1‑135 (MQ=255)
                                                                                               gggggCGCTGACGTCGCTTCCCACCCGGGGGGCCTGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  3:253841/132‑1 (MQ=255)
                                                                                                    cgcAGACTTCGCTCCCCACCCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  6:67571/135‑1 (MQ=255)
                                                                                                    cgcAGACTTCGCGCACCAACCCGGCGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  3:130413/135‑1 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCTGTAGc              >  7:317465/1‑135 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGGGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCGGTAGc              >  4:282886/1‑135 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGc              >  8:77876/1‑135 (MQ=255)
                                                                                                                 gACCAACCCGGCGGCCACGGCGCGATCCGCGCCCCACAACTCGTCGAGGAACAGCACGCGCTTGGCCTGCTCCCAGCCCATCAGCCGTGGCAGGTGCCAGCTGGCCCCGGCATAGGGGCTGTAGCCCAAGCTGgt    >  6:247881/1‑135 (MQ=255)
                                                                                                                   ccACCCGGGGGGCCTGGGCGCTTTGGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:109105/135‑1 (MQ=255)
                                                                                                                   ccACCCGGGCGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:148947/135‑1 (MQ=255)
                                                                                                                   ccACCCGGGCGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:133327/135‑1 (MQ=255)
                                                                                                                   ccACCCCGGCGGCCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:77876/135‑1 (MQ=255)
                                                                                                                   ccAACCGGGCGGCCTGGGCGGGAGGGGCGCCACACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCGGGCGCCGGCATCTGGGCTGTAGCCCATGCTGgtgt  <  4:241428/135‑1 (MQ=255)
                                                                                                                   ccAACCGGGCGGCCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:5839/135‑1 (MQ=255)
                                                                                                                    ccaCCCGGCGGCCCCGGGGGCCTGCGGCCCCCCACACCTCGTCGCGGAACAGCAGGCGCGTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:36371/133‑1 (MQ=255)
                                                                                                                                  |                                                                                                                        
CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245016‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: